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aceE:On One Page

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Gene Name	aceE (synonyms: ECK0113, b0114, JW0110)^[1]
Product(s)	AceE product^[1]: pyruvate dehydrogenase, decarboxylase component E1, thiamin-binding product, B0114 product
Description	subunit of E1p component of pyruvate dehydrogenase complex^[2]^[3] Component of E1P-CPLX^[2],pyruvate dehydrogenase multienzyme complex^[2]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aceEF^[2], pdhR-aceEF-lpdA^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name	aceE
Mnemonic
Synonyms	ECK0113, b0114, JW0110^[1]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	2.65 minutes	MG1655: 123017..125680	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 123017..125680	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

2.65 minutes

MG1655: 123017..125680
gbrowse_img.php?coord=122017..126680&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 123017..125680
gbrowse_img.php?coord=122017..126680&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔaceE (Keio:JW0110)	deletion	deletion	PMID:16738554^[4]	Shigen CGSC8392^[5]
aceE64				CGSC:5645
aceE2				CGSC:8124
ΔaceE732::kan			PMID:16738554^[4]	CGSC:99886
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW0110	Plasmid clone	Shigen	PMID:16769691^[6] Status:Clone OK Primer 1:GCCTCAGAACGTTTCCCAAATGA Primer 2:CCCGCCAGACGCGGGTTAACTTT
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 19% ^[7] Synonyms:zab-3051::Tn10 The start of leuO was reannotated by Riley et al. (2006).
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 38% ^[7]
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10024
EchoBASE (EcoliWiki Page)	EchoBASE:EB0023
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0114
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C0142
EcoGene (EcoliWiki Page)	EcoGene:EG10024
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:ACEE
CGSC (EcoliWiki Page)	CGSC:1050
ASAP (EcoliWikiPage)	ASAP:ABE-0000397
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	AceE
Synonyms	pyruvate dehydrogenase, decarboxylase component E1, thiamin-binding^[1], B0114^[2]^[1], AceE^[2]^[1]
Product description	subunit of E1p component of pyruvate dehydrogenase complex^[2]^[3]; Component of ; E1P-CPLX^[2]; pyruvate dehydrogenase multienzyme complex^[2]^[3]
EC number (for enzymes)	1.2.4.1^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006096	glycolysis	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0324	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0004739	pyruvate dehydrogenase (acetyl-transferring) activity	GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.2.4.1	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0000287	magnesium ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0003824	catalytic activity	GO_REF:0000002 GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR009014 InterPro:IPR015941	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008152	metabolic process	GO_REF:0000002 GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR009014 InterPro:IPR015941	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016491	oxidoreductase activity	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR004660 SP_KW:KW-0560	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0046872	metal ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P05100	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0A8L7	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0A8N7	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0A9U1	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0AB83	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0AB89	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0AEV9	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0AG40	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P26602	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P46889	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P76049	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P76170	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:15690043^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P77439	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:16606699^[10]	IPI: Inferred from Physical Interaction	UniProtKB:P0A6F5	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005624	membrane fraction	PMID:16858726^[11]	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	required fields missing
	GO:0016052	carbohydrate catabolic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0006086	acetyl-CoA biosynthetic process from pyruvate				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0009436	glyoxylate catabolic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0009061	anaerobic respiration				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0004738	pyruvate dehydrogenase activity				F		required field missing
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Interactions

Partner Type	Partner	Notes
Protein	Subunits of E1P-CPLX	could be indirect
Protein	Subunits of pyruvate dehydrogenase multienzyme complex	could be indirect
Small Molecule	TTTPP	selectively inhibits pyruvate or 2-oxoglutarate dehydrogenase (El).
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Notes

Localization

Compartment	Description	Evidence	Notes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MSERFPNDVD PIETRDWLQA IESVIREEGV ERAQYLIDQL LAEARKGGVN VAAGTGISNY INTIPVEEQP EYPGNLELER RIRSAIRWNA IMTVLRASKK DLELGGHMAS FQSSATIYDV CFNHFFRARN EQDGGDLVYF QGHISPGVYA RAFLEGRLTQ EQLDNFRQEV HGNGLSSYPH PKLMPEFWQF PTVSMGLGPI GAIYQAKFLK YLEHRGLKDT SKQTVYAFLG DGEMDEPESK GAITIATREK LDNLVFVINC NLQRLDGPVT GNGKIINELE GIFEGAGWNV IKVMWGSRWD ELLRKDTSGK LIQLMNETVD GDYQTFKSKD GAYVREHFFG KYPETAALVA DWTDEQIWAL NRGGHDPKKI YAAFKKAQET KGKATVILAH TIKGYGMGDA AEGKNIAHQV KKMNMDGVRH IRDRFNVPVS DADIEKLPYI TFPEGSEEHT YLHAQRQKLH GYLPSRQPNF TEKLELPSLQ DFGALLEEQS KEISTTIAFV RALNVMLKNK SIKDRLVPII ADEARTFGME GLFRQIGIYS PNGQQYTPQD REQVAYYKED EKGQILQEGI NELGAGCSWL AAATSYSTNN LPMIPFYIYY SMFGFQRIGD LCWAAGDQQA RGFLIGGTSG RTTLNGEGLQ HEDGHSHIQS LTIPNCISYD PAYAYEVAVI MHDGLERMYG EKQENVYYYI TTLNENYHMP AMPEGAEEGI RKGIYKLETI EGSKGKVQLL GSGSILRHVR EAAEILAKDY GVGSDVYSVT SFTELARDGQ DCERWNMLHP LETPRVPYIA QVMNDAPAVA STDYMKLFAE QVRTYVPADD YRVLGTDGFG RSDSRENLRH HFEVDASYVV VAALGELAKR GEIDKKVVAD AIAKFNIDAD KVNPRLA
Mol. Wt	99.67 kDa (calc) ^[2]
pI	5.76^[2]
Extinction coefficient	123080 - 123830 (calc based on 42 Y, 11 W, and 6 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
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Structure

Structures	Representative Escherichia coli structure: 2QTA\|A 1-886 of 887 residues (E-value: 0.) (Percent Identity: 99.89) Representative homolog: 1TKA\|A (Saccharomyces cerevisiae) 62-219 of 887 residues (E-value: 1.6e-06) (Percent Identity: 29.41) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative Escherichia coli structure:
2QTA|A
1-886 of 887 residues (E-value: 0.) (Percent Identity: 99.89)
Representative homolog:
1TKA|A (Saccharomyces cerevisiae)
62-219 of 887 residues (E-value: 1.6e-06) (Percent Identity: 29.41)
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Models

View models at:

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:E1P-MONOMER
PDB (EcoliWiki Page)	PDB:1RP7
SwissModel (EcoliWiki Page)	SwissModel:P0AFG8
UniProt (EcoliWiki Page)	UniProt:P0AFG8
RefSeq (EcoliWiki Page)	RefSeq:NP_414656
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
aceEF
pdhR-aceEF-lpdA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=122997..123037&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b0114 (EcoliWiki Page)	Summary of data for aceE from multiple microarray studies
microarray	GEO Profiles:b0114 (EcoliWiki Page)	NCBI GEO profiles for aceE
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to aceE Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10024
EchoBASE (EcoliWiki Page)	EchoBASE:EB0023
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b0114
EcoGene (EcoliWiki Page)	EcoGene:EG10024
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000025779 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	ACEE	From SHIGELLACYC
E. coli O157	ACEE	From ECOO157CYC
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Families

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Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000025779 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	ACEE	From SHIGELLACYC
E. coli O157	ACEE	From ECOO157CYC
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Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^8.00 ^8.01 ^8.02 ^8.03 ^8.04 ^8.05 ^8.06 ^8.07 ^8.08 ^8.09 ^8.10 ^8.11 ^8.12 ^8.13 ^8.14 ^8.15 ^8.16 ^8.17 ^8.18 ^8.19 ^8.20 ^8.21 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^9.00 ^9.01 ^9.02 ^9.03 ^9.04 ^9.05 ^9.06 ^9.07 ^9.08 ^9.09 ^9.10 ^9.11 ^9.12 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
↑ Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
↑ Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed EcoliWiki page

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aceE:On One Page

From EcoliWiki

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aceE Expression

Notes

Links

Categories

Evolution

Homologs in Other Organisms

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Notes

Families

Notes

Links

References

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