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aceK:On One Page

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Gene Name	aceK (synonyms: ECK4008, b4016, JW3976)^[1]
Product(s)	AceK product^[1]: isocitrate dehydrogenase kinase/phosphatase product, B4016 product
Description	AceK^[2]^[3]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aceBAK^[2], OP00001, ace
Regulation/Activity
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Gene

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Nomenclature

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Standard name	aceK
Mnemonic
Synonyms	ECK4008, b4016, JW3976^[1]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	90.88 minutes	MG1655: 4216619..4218355	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 4222186..4223922	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

90.88 minutes

MG1655: 4216619..4218355
gbrowse_img.php?coord=4215619..4219355&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 4222186..4223922
gbrowse_img.php?coord=4221186..4224922&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔaceK (Keio:JW3976)	deletion	deletion	PMID:16738554^[4]	Shigen CGSC10859^[5]
ΔaceK783::kan			PMID:16738554^[4]	CGSC:99890
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW3976	Plasmid clone	Shigen	PMID:16769691^[6] Status:Clone OK Primer 1:GCCCCGCGTGGCCTGGAATTATT Primer 2:CCAAAAAGCATCTCCCCATACCG
thiC39::Tn10	Linked marker	CAG18500 = CGSC7472^[5]	est. P1 cotransduction: 44% ^[7] Synonyms:thi-39::Tn10
malF3089::Tn10	Linked marker	CAG12164 = CGSC7476^[5]	est. P1 cotransduction: 41% ^[7]
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10026
EchoBASE (EcoliWiki Page)	EchoBASE:EB0025
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4016
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C4974
EcoGene (EcoliWiki Page)	EcoGene:EG10026
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:ACEK
CGSC (EcoliWiki Page)	CGSC:17770
ASAP (EcoliWikiPage)	ASAP:ABE-0013131
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	AceK
Synonyms	isocitrate dehydrogenase kinase/phosphatase^[1], B4016^[2]^[1], AceK^[2]^[1]
Product description	AceK^[2]^[3]
EC number (for enzymes)	2.7.1.116^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0003824	catalytic activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0511	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0004721	phosphoprotein phosphatase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-904	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006097	glyoxylate cycle	GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	SP_KW:KW-0329 HAMAP:MF_00747	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006006	glucose metabolic process	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010452	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005524	ATP binding	GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067 HAMAP:MF_00747	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005737	cytoplasm	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR010452 SP_KW:KW-0963	C	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006099	tricarboxylic acid cycle	GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	SP_KW:KW-0816 HAMAP:MF_00747	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008772	(isocitrate dehydrogenase (NADP+)) kinase activity	GO_REF:0000002 GO_REF:0000003 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR010452 EC:2.7.11.5 HAMAP:MF_00747	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016301	kinase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0418	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016740	transferase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016787	hydrolase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016788	hydrolase activity, acting on ester bonds	GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00747	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016791	phosphatase activity	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010452	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006464	protein modification process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0016485	protein processing				P	Seeded from EcoCyc 11.1^[3].	required fields missing
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Interactions

Partner Type	Partner	Notes	References
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Notes

Localization

Compartment	Description	Evidence	Notes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MPRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEQADW HAVQQAMKNR IHLYDHHVGL VVEQLRCITN GQSTDAAFLL RVKEHYTRLL PDYPRFEIAE SFFNSVYCRL FDHRSLTPER LFIFSSQPER RFRTIPRPLA KDFHPDHGWE SLLMRVISDL PLRLRWQNKS RDIHYIIRHL TETLGTDNLA ESHLQVANEL FYRNKAAWLV GKLITPSGTL PFLLPIHQTD DGELFIDTCL TTTAEASIVF GFARSYFMVY APLPAALVEW LREILPGKTT AELYMAIGCQ KHAKTESYRE YLVYLQGCNE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD RFAPQKEMSA AHVRACYQLV KEHDRVGRMA DTQEFENFVL EKRHISPALM ELLLQEAAEK ITDLGEQIVI RHLYIERRMV PLNIWLEQVE GQQLRDAIEE YGNAIRQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC YMTEVNFRDI PPPRYPEDEL ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHADLF RADYWRALQN RIREGHVEDV YAYRRRQRFS VRYGEMLF
Mol. Wt	67.7 kDa (calc) ^[2]
pI
Extinction coefficient	86750 - 87750 (calc based on 25 Y, 9 W, and 8 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
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Structure

Structures	No results found.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

No results found.

Models

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:ICITDEHASE-KIN-PHOSPHA
UniProt (EcoliWiki Page)	UniProt:P11071
PFAM (EcoliWiki Page)	PFAM:PF06315
RefSeq (EcoliWiki Page)	RefSeq:NP_418440
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
aceBAK
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=4216599..4216639&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b4016 (EcoliWiki Page)	Summary of data for aceK from multiple microarray studies
microarray	GEO Profiles:b4016 (EcoliWiki Page)	NCBI GEO profiles for aceK
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4215922..4216245) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ18; Well:A10^[9]
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Notes

Accessions Related to aceK Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10026
EchoBASE (EcoliWiki Page)	EchoBASE:EB0025
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b4016
EcoGene (EcoliWiki Page)	EcoGene:EG10026
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF06315
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Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^8.00 ^8.01 ^8.02 ^8.03 ^8.04 ^8.05 ^8.06 ^8.07 ^8.08 ^8.09 ^8.10 ^8.11 ^8.12 ^8.13 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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Retrieved from "http://ecoliwiki.net/colipedia/index.php/aceK:On_One_Page"

aceK:On One Page

From EcoliWiki

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aceK Expression

Notes

Links

Categories

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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