Standard name	AcnA
Synonyms	aconitate hydratase 1^[1], B1276^[2]^[1], Acn^[2]^[1], AcnA^[2]^[1]
Product description	ACONITASE^[2]^[3]
EC number (for enzymes)	4.2.1.3^[1]
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Notes

Function

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Gene Ontology
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Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006099	tricarboxylic acid cycle	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0816	P	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0005506	iron ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0003994	aconitate hydratase activity	GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.1.3	F	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0016829	lyase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0046872	metal ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0008152	metabolic process	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000573 InterPro:IPR001030 InterPro:IPR006249 InterPro:IPR015928 InterPro:IPR015931 InterPro:IPR015932 InterPro:IPR015934 InterPro:IPR015937	P	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0051539	4 iron, 4 sulfur cluster binding	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR006249 SP_KW:KW-0004	F	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0051536	iron-sulfur cluster binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0411	F	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P18843	F	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0006979	response to oxidative stress	PMID:9421904^[6]	IEP: Inferred from Expression Pattern		P	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0043556	regulation of translation in response to oxidative stress	PMID:10589714^[7]	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	required fields missing
	GO:0003994	aconitate hydratase activity	PMID:1838390^[8]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc 12.5 ^[4]	complete
	GO:0006099	tricarboxylic acid cycle	PMID:13152052^[9]	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc 11.1^[3].	complete
	GO:0009061	anaerobic respiration				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0006979	response to oxidative stress	PMID:9421904^[6]	IEP: Inferred from Expression Pattern		P	Seeded from EcoCyc 11.1^[3].	complete
	GO:0003994	aconitate hydratase activity	PMID:1838390^[8]	IDA: Inferred from Direct Assay		F		complete
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Interactions

Partner Type	Partner	Notes	References
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Notes

Localization

Compartment	Description	Evidence	Notes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MSSTLREASK DTLQAKDKTY HYYSLPLAAK SLGDITRLPK SLKVLLENLL RWQDGNSVTE EDIHALAGWL KNAHADREIA YRPARVLMQD FTGVPAVVDL AAMREAVKRL GGDTAKVNPL SPVDLVIDHS VTVDRFGDDE AFEENVRLEM ERNHERYVFL KWGKQAFSRF SVVPPGTGIC HQVNLEYLGK AVWSELQDGE WIAYPDTLVG TDSHTTMING LGVLGWGVGG IEAEAAMLGQ PVSMLIPDVV GFKLTGKLRE GITATDLVLT VTQMLRKHGV VGKFVEFYGD GLDSLPLADR ATIANMSPEY GATCGFFPID AVTLDYMRLS GRSEDQVELV EKYAKAQGMW RNPGDEPIFT STLELDMNDV EASLAGPKRP QDRVALPDVP KAFAASNELE VNATHKDRQP VDYVMNGHQY QLPDGAVVIA AITSCTNTSN PSVLMAAGLL AKKAVTLGLK RQPWVKASLA PGSKVVSDYL AKAKLTPYLD ELGFNLVGYG CTTCIGNSGP LPDPIETAIK KSDLTVGAVL SGNRNFEGRI HPLVKTNWLA SPPLVVAYAL AGNMNINLAS EPIGHDRKGD PVYLKDIWPS AQEIARAVEQ VSTEMFRKEY AEVFEGTAEW KGINVTRSDT YGWQEDSTYI RLSPFFDEMQ ATPAPVEDIH GARILAMLGD SVTTDHISPA GSIKPDSPAG RYLQGRGVER KDFNSYGSRR GNHEVMMRGT FANIRIRNEM VPGVEGGMTR HLPDSDVVSI YDAAMRYKQE QTPLAVIAGK EYGSGSSRDW AAKGPRLLGI RVVIAESFER IHRSNLIGMG ILPLEFPQGV TRKTLGLTGE EKIDIGDLQN LQPGATVPVT LTRADGSQEV VPCRCRIDTA TELTYYQNDG ILHYVIRNML K
Mol. Wt	97.68 kDa (calc) ^[2]
pI
Extinction coefficient	114710 - 115585 (calc based on 29 Y, 13 W, and 7 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
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Structure

Structures	Representative homolog: 2IPY\|A (Oryctolagus cuniculus) 33-888 of 891 residues (E-value: 1.1e-239) (Percent Identity: 53.29) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative homolog:
2IPY|A (Oryctolagus cuniculus)
33-888 of 891 residues (E-value: 1.1e-239) (Percent Identity: 53.29)
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Models

View models at:

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:ACONITASE-MONOMER
UniProt (EcoliWiki Page)	UniProt:P25516
PFAM (EcoliWiki Page)	PFAM:PF00330
RefSeq (EcoliWiki Page)	RefSeq:NP_415792
ModBase (EcoliWiki Page)	ModBase:P25516
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Notes

Links

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Name	URL	Comments
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References

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↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.00 ^4.01 ^4.02 ^4.03 ^4.04 ^4.05 ^4.06 ^4.07 ^4.08 ^4.09 ^4.10 ^4.11 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
↑ ^6.0 ^6.1 Cunningham L et al. (1997) Transcriptional regulation of the aconitase genes (acnA and acnB) of Escherichia coli. Microbiology 143 ( Pt 12): 3795-805 PubMed EcoliWiki page
↑ Tang Y & Guest JR (1999) Direct evidence for mRNA binding and post-transcriptional regulation by Escherichia coli aconitases. Microbiology 145 ( Pt 11): 3069-79 PubMed EcoliWiki page
↑ ^8.0 ^8.1 Prodromou C et al. (1991) The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn). J Gen Microbiol 137: 2505-15 PubMed EcoliWiki page
↑ SWIM HE & KRAMPITZ LO (1954) Acetic acid oxidation by Escherichia coli; evidence for the occurrence of a tricarboxylic acid cycle. J Bacteriol 67: 419-25 PubMed EcoliWiki page

acnA:Gene Product(s)

From EcoliWiki

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

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