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acpT:On One Page

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Gene Name	acpT (synonyms: ECK3459, b3475, JW3440, yhhU)^[1]
Product(s)	AcpT product^[1]: holo-(acyl carrier protein) synthase 2 product, B3475 product, YhhU product
Description	holo-[acyl carrier protein] synthase 2^[2]^[3]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): acpT^[2]
Regulation/Activity
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Gene

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Nomenclature

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Standard name	acpT
Mnemonic
Synonyms	ECK3459, b3475, JW3440, yhhU^[1]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	77.83 minutes	MG1655: 3610992..3611579	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 4027446..4026859	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

77.83 minutes

MG1655: 3610992..3611579
gbrowse_img.php?coord=3609992..3612579&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 4027446..4026859
gbrowse_img.php?coord=4025859..4028446&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔacpT (Keio:JW3440)	deletion	deletion			PMID:16738554^[4]	Shigen
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW3440	Plasmid clone	Shigen	PMID:16769691^[5] Status:Clone OK Primer 1:GCCTATCGGATAGTTCTGGGGAA Primer 2:CCGTTAACTGAATCGATCCATTG
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[6]	est. P1 cotransduction: 49% ^[7] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[6]	est. P1 cotransduction: % ^[7]
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG12221
EchoBASE (EcoliWiki Page)	EchoBASE:EB2135
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3475
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C4268
EcoGene (EcoliWiki Page)	EcoGene:EG12221
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:Z4867
ASAP (EcoliWikiPage)	ASAP:ABE-0011351
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	AcpT
Synonyms	holo-(acyl carrier protein) synthase 2^[1], B3475^[2]^[1], YhhU^[2]^[1], AcpT^[2]^[1]
Product description	holo-[acyl carrier protein] synthase 2^[2]^[3]
EC number (for enzymes)	2.7.8.7^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0009059	macromolecule biosynthetic process	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008278	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008278	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0000287	magnesium ion binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008278	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016740	transferase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	PMID:8939709^[9]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	required fields missing
	GO:0006654	phosphatidic acid biosynthetic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
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Interactions

Partner Type	Partner	Notes	References
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Notes

Localization

Compartment	Description	Evidence	Notes
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Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MYRIVLGKVS TLSAAPLPPG LREQAPQGPR RERWLAGRAL LSHTLSPLPE IIYGEQGKPA FAPEMPLWFN LSHSGDDIAL LLSDEGEVGC DIEVIRPRAN WRWLANAVFS LGEHAEMDAV HPDQQLEMFW RIWTRKEAIV KQRGGSAWQI VSVDSTYHSS LSVSHCQLEN LSLAICTPTP FTLTADSVQW IDSVN
Mol. Wt	21.77 kDa (calc) ^[2]
pI
Extinction coefficient	48470 - 48845 (calc based on 3 Y, 8 W, and 3 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
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Structure

Structures	Representative homolog: 1QR0\|A (Bacillus subtilis) 72-179 of 195 residues (E-value: 2.7e-09) (Percent Identity: 33.04) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative homolog:
1QR0|A (Bacillus subtilis)
72-179 of 195 residues (E-value: 2.7e-09) (Percent Identity: 33.04)
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Models

View models at:

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG12221-MONOMER
UniProt (EcoliWiki Page)	UniProt:P37623
PFAM (EcoliWiki Page)	PFAM:PF01648
RefSeq (EcoliWiki Page)	RefSeq:NP_417932
ModBase (EcoliWiki Page)	ModBase:P37623
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
acpT
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=3610972..3611012&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b3475 (EcoliWiki Page)	Summary of data for acpT from multiple microarray studies
microarray	GEO Profiles:b3475 (EcoliWiki Page)	NCBI GEO profiles for acpT
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to acpT Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG12221
EchoBASE (EcoliWiki Page)	EchoBASE:EB2135
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b3475
EcoGene (EcoliWiki Page)	EcoGene:EG12221
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Shigella flexneri	YHHU	From SHIGELLACYC
E. coli O157	Z4867	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF01648
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Links

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Name	URL	Comments
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References

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↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^6.0 ^6.1 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^8.0 ^8.1 ^8.2 ^8.3 ^8.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Lambalot RH et al. (1996) A new enzyme superfamily - the phosphopantetheinyl transferases. Chem Biol 3: 923-36 PubMed EcoliWiki page

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Retrieved from "http://ecoliwiki.net/colipedia/index.php/acpT:On_One_Page"

acpT:On One Page

From EcoliWiki

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to acpT Expression

Notes

Links

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Evolution

Homologs in Other Organisms

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Notes

Families

Notes

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