acrA:Gene - EcoliWiki
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acrA:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

acrA

Mnemonic
Synonyms

ECK0457, b0463, JW0452, Mb, lir, mbl, mtcA, sipB, nbsA[1]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

10.42 minutes 

MG1655: 484843..483650
gbrowse_img.php?coord=482650..485843&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 484843..483650
gbrowse_img.php?coord=482650..485843&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔacrA (Keio:JW0452)

deletion

deletion

PMID:16738554[2]

Shigen

acrA1

CGSC:6294

acrA13

CGSC:7201


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Notes

AcrA mutants grow to higher cell density than wt [3]

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW0452

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAACAAAAACAGAGGGTTTAC

Primer 2:CCAGACTTGGACTGTTCAGGCTG

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[5]

est. P1 cotransduction: 40% [6]

Synonyms:zba-3054::Tn10

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[5]

est. P1 cotransduction: 54% [6]

Synonyms:zbb-30-55::TnlO, zba-3055::Tn10

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11703

EchoBASE (EcoliWiki Page)

EchoBASE:EB1654

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0463

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0581

EcoGene (EcoliWiki Page)

EcoGene:EG11703

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ACRA

CGSC (EcoliWiki Page)

CGSC:1045

ASAP (EcoliWikiPage)

ASAP:ABE-0001603


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. Yang S et al. (2006) Quorum sensing and multidrug transporters in Escherichia coli. Proc Natl Acad Sci U S A 103: 2386-91 PubMed EcoliWiki page
  4. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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