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acrE:On One Page

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Gene Name	acrE (synonyms: ECK3252, b3265, JW3233, G7979)^[1]^[2]
Product(s)	AcrE product^[1]: cytoplasmic membrane lipoprotein product, B3265 product
Description	transmembrane protein affects septum formation and cell membrane permeability^[2]^[3] Component of AcrEF-TolC Multidrug Efflux Transport System^[3]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): acrEF^[2]
Regulation/Activity
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Gene

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Nomenclature

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Standard name	acrE
Mnemonic
Synonyms	ECK3252, b3265, JW3233, G7979^[1]^[2]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	73.54 minutes	MG1655: 3411886..3413043	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 3413719..3414876	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

73.54 minutes

MG1655: 3411886..3413043
gbrowse_img.php?coord=3410886..3414043&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 3413719..3414876
gbrowse_img.php?coord=3412719..3415876&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔacrE (Keio:JW3233)	deletion	deletion			PMID:16738554^[4]	Shigen
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW3233	Plasmid clone	Shigen	PMID:16769691^[5] Status:Clone OK Primer 1:GCCACGAAACATGCCAGGTTTTT Primer 2:CCtTTCGATGCAGTATCTGCGGG
zhc-6::Tn10	Linked marker	CAG12153 = CGSC7442^[6]	est. P1 cotransduction: 11% ^[7] Synonyms:zha-6::Tn10
acrF3083::Tn10	Linked marker	CAG12075 = CGSC7447^[6]	est. P1 cotransduction: 91% ^[7] Synonyms:zhd-3083::Tn10, zhe-3083::Tn10
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10266
EchoBASE (EcoliWiki Page)	EchoBASE:EB0262
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3265
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C4031
EcoGene (EcoliWiki Page)	EcoGene:EG10266
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:ACRE
CGSC (EcoliWiki Page)	CGSC:813
ASAP (EcoliWikiPage)	ASAP:ABE-0010722
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	AcrE
Synonyms	cytoplasmic membrane lipoprotein^[1], B3265^[2]^[1], AcrE^[2]^[1]
Product description	transmembrane protein affects septum formation and cell membrane permeability^[2]^[3]; Component of ; AcrEF-TolC Multidrug Efflux Transport System^[3]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0009306	protein secretion	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016020	membrane	GO_REF:0000002 GO_REF:0000004 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR006143 SP_KW:KW-0397 SP_KW:KW-0472	C	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0007049	cell cycle	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0131	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008565	protein transporter activity	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0051301	cell division	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0132	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0042493	response to drug	PMID:8407802^[9]	ISS: Inferred from Sequence or Structural Similarity	EcoliWiki:AcrF	P		complete
	GO:0031226	intrinsic to plasma membrane	PMID:8407802^[9]	IMP: Inferred from Mutant Phenotype		C		complete
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Interactions

Partner Type	Partner	Notes	References
Protein	Subunits of AcrEF-TolC Multidrug Efflux Transport System	could be indirect
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Notes

Localization

Compartment	Description	Evidence	Notes
plasma membrane
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Notes

Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MTKHARFFLL PSFILISAAL IAGCNDKGEE KAHVGEPQVT VHIVKTAPLE VKTELPGRTN AYRIAEVRPQ VSGIVLNRNF TEGSDVQAGQ SLYQIDPATY QANYDSAKGE LAKSEAAAAI AHLTVKRYVP LVGTKYISQQ EYDQAIADAR QADAAVIAAK ATVESARINL AYTKVTAPIS GRIGKSTVTE GALVTNGQTT ELATVQQLDP IYVDVTQSSN DFMRLKQSVE QGNLHKENAT SNVELVMENG QTYPLKGTLQ FSDVTVDEST GSITLRAVFP NPQHTLLPGM FVRARIDEGV QPDAILIPQQ GVSRTPRGDA TVLIVNDKSQ VEARPVVASQ AIGDKWLISE GLKSGDQVIV SGLQKARPGE QVKATTDTPA DTASK
Mol. Wt	41.32 kDa (calc) ^[2]
pI
Extinction coefficient	20400 - 20525 (calc based on 10 Y, 1 W, and 1 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
motif	1-23	UniProt Manual:Signal Peptides	UniProt:P24180
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Structure

Structures	Representative homolog: 1VF7\|A (Pseudomonas aeruginosa) 1-351 of 385 residues (E-value: 2.8e-94) (Percent Identity: 53.28) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative homolog:
1VF7|A (Pseudomonas aeruginosa)
1-351 of 385 residues (E-value: 2.8e-94) (Percent Identity: 53.28)
View all other structures.

Models

View models at:

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10266-MONOMER
SwissModel (EcoliWiki Page)	SwissModel:P24180
UniProt (EcoliWiki Page)	UniProt:P24180
PFAM (EcoliWiki Page)	PFAM:PF00529
RefSeq (EcoliWiki Page)	RefSeq:NP_417731
ModBase (EcoliWiki Page)	ModBase:P24180
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
acrEF
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=3411866..3411906&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b3265 (EcoliWiki Page)	Summary of data for acrE from multiple microarray studies
microarray	GEO Profiles:b3265 (EcoliWiki Page)	NCBI GEO profiles for acrE
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to acrE Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10266
EchoBASE (EcoliWiki Page)	EchoBASE:EB0262
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b3265
EcoGene (EcoliWiki Page)	EcoGene:EG10266
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000001354 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000051131 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	ACRE	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF00529
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Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^6.0 ^6.1 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^8.0 ^8.1 ^8.2 ^8.3 ^8.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^9.0 ^9.1 Ma D et al. (1993) Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. J Bacteriol 175: 6299-313 PubMed EcoliWiki page

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Retrieved from "http://ecoliwiki.net/colipedia/index.php/acrE:On_One_Page"

acrE:On One Page

From EcoliWiki

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to acrE Expression

Notes

Links

Categories

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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