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acrR:On One Page

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Gene Name	acrR (synonyms: ECK0458, b0464, JW0453, ybaH)^[1]
Product(s)	AcrR product^[1]: DNA-binding transcriptional repressor product, B0464 product, YbaH product, negative DNA-binding transcriptional regulator for multidrug efflux pump product
Description	AcrR transcriptional regulator^[2] AcrR transcriptional repressor^[3]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): acrR^[2]
Regulation/Activity
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Gene

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Nomenclature

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Standard name	acrR
Mnemonic
Synonyms	ECK0458, b0464, JW0453, ybaH^[1]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	10.45 minutes	MG1655: 484985..485632	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 484985..485632	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

10.45 minutes

MG1655: 484985..485632
gbrowse_img.php?coord=483985..486632&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 484985..485632
gbrowse_img.php?coord=483985..486632&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔacrR (Keio:JW0453)	deletion	deletion			PMID:16738554^[4]	Shigen
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW0453	Plasmid clone	Shigen	PMID:16769691^[5] Status:Clone OK Primer 1:GCCGCACGAAAAACCAAACAAGA Primer 2:CCTTCGTTAGTGGCAGGATTACG
zaj-3054::Tn10	Linked marker	CAG12017 = CGSC7339^[6]	est. P1 cotransduction: 38% ^[7] Synonyms:zba-3054::Tn10
gsk-3055::Tn10	Linked marker	CAG12154 = CGSC7341^[6]	est. P1 cotransduction: 56% ^[7] Synonyms:zbb-30-55::TnlO, zba-3055::Tn10
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG12116
EchoBASE (EcoliWiki Page)	EchoBASE:EB2039
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0464
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C0582
EcoGene (EcoliWiki Page)	EcoGene:EG12116
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:ACRR
CGSC (EcoliWiki Page)	CGSC:35809
ASAP (EcoliWikiPage)	ASAP:ABE-0001608
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	AcrR
Synonyms	DNA-binding transcriptional repressor^[1], B0464^[2]^[1], YbaH^[2]^[1], AcrR^[2]^[1], negative DNA-binding transcriptional regulator for multidrug efflux pump^[2]^[1]
Product description	AcrR transcriptional regulator^[2]; AcrR transcriptional repressor^[3]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR012287 InterPro:IPR013572 SP_KW:KW-0238	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0003700	transcription factor activity	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001647	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006350	transcription	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006355	regulation of transcription, DNA-dependent	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR001647 SP_KW:KW-0805	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0045449	regulation of transcription	GO_REF:0000002 GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011075 InterPro:IPR012287	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P13035	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006350	transcription	PMID:8821940^[10]	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006350	transcription	PMID:8821940^[10]	IEP: Inferred from Expression Pattern		P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006350	transcription	PMID:8821940^[10]	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016564	transcription repressor activity	PMID:8821940^[10]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016564	transcription repressor activity	PMID:8821940^[10]	IEP: Inferred from Expression Pattern		F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016564	transcription repressor activity	PMID:8821940^[10]	IMP: Inferred from Mutant Phenotype		F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0042493	response to drug				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0005737	cytoplasm				C	Seeded from EcoCyc 11.1^[3].	required fields missing
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Interactions

Partner Type	Partner	Notes	References
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Notes

Localization

Compartment	Description	Evidence	Notes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MARKTKQEAQ ETRQHILDVA LRLFSQQGVS STSLGEIAKA AGVTRGAIYW HFKDKSDLFS EIWELSESNI GELELEYQAK FPGDPLSVLR EILIHVLEST VTEERRRLLM EIIFHKCEFV GEMAVVQQAQ RNLCLESYDR IEQTLKHCIE AKMLPADLMT RRAAIIMRGY ISGLMENWLF APQSFDLKKE ARDYVAILLE MYLLCPTLRN PATNE
Mol. Wt	24.77 kDa (calc) ^[2]
pI
Extinction coefficient	25440 - 25940 (calc based on 6 Y, 3 W, and 4 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
Domain	10 - 70	HTH tetR-type	UniProt:P0ACS9
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Structure

Structures	Representative Escherichia coli structure: 2QOP\|A 1-215 of 215 residues (E-value: 7.1e-112) (Percent Identity: 100.00) Representative homolog: 1UI6\|A (Streptomyces coelicolor) 1-63 of 215 residues (E-value: 1.2e-08) (Percent Identity: 46.15) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative Escherichia coli structure:
2QOP|A
1-215 of 215 residues (E-value: 7.1e-112) (Percent Identity: 100.00)
Representative homolog:
1UI6|A (Streptomyces coelicolor)
1-63 of 215 residues (E-value: 1.2e-08) (Percent Identity: 46.15)
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Models

View models at:

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG12116-MONOMER
SwissModel (EcoliWiki Page)	SwissModel:P0ACS9
UniProt (EcoliWiki Page)	UniProt:P0ACS9
PFAM (EcoliWiki Page)	PFAM:PF00440
RefSeq (EcoliWiki Page)	RefSeq:NP_414997
ModBase (EcoliWiki Page)	ModBase:P0ACS9
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
acrR
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=484965..485005&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b0464 (EcoliWiki Page)	Summary of data for acrR from multiple microarray studies
microarray	GEO Profiles:b0464 (EcoliWiki Page)	NCBI GEO profiles for acrR
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to acrR Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG12116
EchoBASE (EcoliWiki Page)	EchoBASE:EB2039
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b0464
EcoGene (EcoliWiki Page)	EcoGene:EG12116
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000024011 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	ACRR	From SHIGELLACYC
E. coli O157	ACRR	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF00440
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Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^6.0 ^6.1 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^8.00 ^8.01 ^8.02 ^8.03 ^8.04 ^8.05 ^8.06 ^8.07 ^8.08 ^8.09 ^8.10 ^8.11 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
↑ ^10.0 ^10.1 ^10.2 ^10.3 ^10.4 ^10.5 Ma D et al. (1996) The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals. Mol Microbiol 19: 101-12 PubMed EcoliWiki page

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acrR:On One Page

From EcoliWiki

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to acrR Expression

Notes

Links

Categories

Evolution

Homologs in Other Organisms

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Notes

Families

Notes

Links

References

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