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actP:On One Page

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Gene Name	actP (synonyms: ECK4060, b4067, JW4028, yjcG)^[1]
Product(s)	ActP product^[1]: acetate transporter product, B4067 product, YjcG product
Description	YjcG SSS transporter^[2]^[3]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): acs-yjcHG^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name	actP
Mnemonic
Synonyms	ECK4060, b4067, JW4028, yjcG^[1]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	92.28 minutes	MG1655: 4282925..4281276	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 4288492..4286843	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

92.28 minutes

MG1655: 4282925..4281276
gbrowse_img.php?coord=4280276..4283925&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 4288492..4286843
gbrowse_img.php?coord=4285843..4289492&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔactP (Keio:JW4028)	deletion	deletion	PMID:16738554^[4]	Shigen CGSC10896^[5]
ΔactP761::kan			PMID:16738554^[4]	CGSC:99906
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW4028	Plasmid clone	Shigen	PMID:16769691^[6] Status:Clone OK Primer 1:GCCAAAAGAGTTCTGACGGCGCT Primer 2:CCATGCGCGCGGCCTTGCTCAAC
malF3089::Tn10	Linked marker	CAG12164 = CGSC7476^[5]	est. P1 cotransduction: 18% ^[7]
zje-2241::Tn10	Linked marker	CAG18427 = CGSC7481^[5]	est. P1 cotransduction: 40% ^[7]
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11942
EchoBASE (EcoliWiki Page)	EchoBASE:EB1886
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4067
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C5062
EcoGene (EcoliWiki Page)	EcoGene:EG11942
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:YJCG
ASAP (EcoliWikiPage)	ASAP:ABE-0013328
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	ActP
Synonyms	acetate transporter^[1], B4067^[2]^[1], YjcG^[2]^[1], ActP^[2]^[1]
Product description	YjcG SSS transporter^[2]^[3]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0016021	integral to membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0031402	sodium ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0915	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0016020	membrane	GO_REF:0000002 GO_REF:0000004 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR001734 SP_KW:KW-0397 SP_KW:KW-0472	C	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0015293	symporter activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0769	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0015123	acetate transmembrane transporter activity	GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01426	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006814	sodium ion transport	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0739	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006811	ion transport	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0406	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0006810	transport	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR001734 SP_KW:KW-0813	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005215	transporter activity	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001734	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0015292	uniporter activity				F	Seeded from EcoCyc 11.1^[3].	required fields missing
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Interactions

Partner Type	Partner	Notes	References
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Notes

Localization

Compartment	Description	Evidence	Notes
plasma membrane	C-terminus localized in the cytoplasm with 13 predicted transmembrane domains	Daley et al. (2005) ^[9]/>
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MKRVLTALAA TLPFAANAAD AISGAVERQP TNWQAIIMFL IFVVFTLGIT YWASKRVRSR SDYYTAGGNI TGFQNGLAIA GDYMSAASFL GISALVFTSG YDGLIYSLGF LVGWPIILFL IAERLRNLGR YTFADVASYR LKQGPIRILS ACGSLVVVAL YLIAQMVGAG KLIELLFGLN YHIAVVLVGV LMMMYVLFGG MLATTWVQII KAVLLLFGAS FMAFMVMKHV GFSFNNLFSE AMAVHPKGVD IMKPGGLVKD PISALSLGLG LMFGTAGLPH ILMRFFTVSD AREARKSVFY ATGFMGYFYI LTFIIGFGAI MLVGANPEYK DAAGHLIGGN NMAAVHLANA VGGNLFLGFI SAVAFATILA VVAGLTLAGA SAVSHDLYAN VFKKGATERE ELRVSKITVL ILGVIAIILG VLFENQNIAF MVGLAFAIAA SCNFPIILLS MYWSKLTTRG AMMGGWLGLI TAVVLMILGP TIWVQILGHE KAIFPYEYPA LFSITVAFLG IWFFSATDNS AEGARERELF RAQFIRSQTG FGVEQGRAH
Mol. Wt	59.2 kDa (calc) ^[2]
pI
Extinction coefficient	72310 - 72560 (calc based on 19 Y, 8 W, and 2 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
Domain	183 - 205	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	212 - 234	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	261 - 283	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	303 - 325	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	359 - 381	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	402 - 424	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	428 - 450	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	462 - 484	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	494 - 516	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	146 - 168	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	76 - 98	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	103 - 125	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
Domain	33 - 55	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[10]' />
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Click the image to view raw output from TMHMM.

Structure

Structures	Representative homolog: 3DH4\|A (Vibrio parahaemolyticus) 18-430 of 549 residues (E-value: 6.3e-13) (Percent Identity: 20.00) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative homolog:
3DH4|A (Vibrio parahaemolyticus)
18-430 of 549 residues (E-value: 6.3e-13) (Percent Identity: 20.00)
View all other structures.

Models

View models at:

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:YJCG-MONOMER
UniProt (EcoliWiki Page)	UniProt:P32705
PFAM (EcoliWiki Page)	PFAM:PF00474
RefSeq (EcoliWiki Page)	RefSeq:NP_418491
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
acs-yjcHG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=4282905..4282945&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled+flip&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b4067 (EcoliWiki Page)	Summary of data for actP from multiple microarray studies
microarray	GEO Profiles:b4067 (EcoliWiki Page)	NCBI GEO profiles for actP
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to actP Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11942
EchoBASE (EcoliWiki Page)	EchoBASE:EB1886
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b4067
EcoGene (EcoliWiki Page)	EcoGene:EG11942
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000017388 (score: 1.000; bootstrap: 100%) ENSANGP00000017405 (score: 0.501)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00028503 (score: 1.000; bootstrap: 56%) WBGene00025216 (score: 0.060)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00014092 (score: 1.000; bootstrap: 57%) WBGene00018716 (score: 0.056)	From Inparanoid:20070104
Drosophila pseudoobscura	GA21946-PA (score: 1.000; bootstrap: 65%) GA21442-PA (score: 0.273) GA15290-PA (score: 0.223) GA17054-PA (score: 0.213) GA21420-PA (score: 0.172) GA20543-PA (score: 0.161)	From Inparanoid:20070104
Oryza gramene	Q9FRN4 (score: 1.000; bootstrap: 100%) Q7XBS0 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC8693c (score: 1.000; bootstrap: 100%) SPBPB8B62c (score: 0.454) SPBC23G73c (score: 0.352)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000014194 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YJCG	From SHIGELLACYC
E. coli O157	YJCG	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF00474
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Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^8.0 ^8.1 ^8.2 ^8.3 ^8.4 ^8.5 ^8.6 ^8.7 ^8.8 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Daley DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308: 1321-3 PubMed EcoliWiki page
↑ ^10.00 ^10.01 ^10.02 ^10.03 ^10.04 ^10.05 ^10.06 ^10.07 ^10.08 ^10.09 ^10.10 ^10.11 ^10.12 Krogh A et al. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305: 567-80 PubMed EcoliWiki page

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Retrieved from "http://ecoliwiki.net/colipedia/index.php/actP:On_One_Page"

actP:On One Page

From EcoliWiki

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to actP Expression

Notes

Links

Categories

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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