E. coli is indeed the coolest thing on the planet - Carl Zimmer

adhE:On One Page

From EcoliWiki

Jump to: navigation, search

Quickview

Gene

Gene Product(s)

Expression

Evolution

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

Gene Name	adhE (synonyms: ECK1235, b1241, JW1228, adhC, ana)^[1]
Product(s)	AdhE product^[1]: fused acetaldehyde-CoA dehydrogenase product, iron-dependent alcohol dehydrogenase product, pyruvate-formate lyase deactivase product, B1241 product, AdhC product, Ana product
Description	AdhE^[2]^[3] Component of ADHE-CPLX^[2]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): adhE^[2], OP00278
Regulation/Activity
edit table

See Help:Quickview for help with entering information in the Quickview table.

Notes

[back to top]

Gene

[back to top]

Nomenclature

[back to top]

Standard name	adhE
Mnemonic
Synonyms	ECK1235, b1241, JW1228, adhC, ana^[1]
edit table

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

[back to top]

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	27.9 minutes	MG1655: 1297344..1294669	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 1299698..1297023	GBrowse(W3110)	FASTA Dump
edit table

Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

27.9 minutes

MG1655: 1297344..1294669
gbrowse_img.php?coord=1293669..1298344&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 1299698..1297023
gbrowse_img.php?coord=1296023..1300698&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

edit table

See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

[back to top]

Feature type	Location	Description
edit table

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

[back to top]

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability
ΔadhE (Keio:JW1228)	deletion	deletion		PMID:16738554^[4]	Shigen CGSC9113^[5]
ana-1					CGSC:5096
adhE80				PMID:6986356^[6]	CGSC:7028
adhE1				PMID:6752127^[7]	CGSC:7285
adhE16(ts)			temperature sensitive		CGSC:7289
adhE2				PMID:6752127^[7]	CGSC:7457
ana-2			adhE		CGSC:14693
adhE36				PMID:3550385^[8]	CGSC:92529
adhE38				PMID:3550385^[8]	CGSC:92530
ΔadhE748::kan				PMID:16738554^[4]	CGSC:99914
edit table

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

[back to top]

Resource	Resource Type	Source	Notes/Reference
JW1228	Plasmid clone	Shigen	PMID:16769691^[9] Status:Clone OK Primer 1:GCCGCTGTTACTAATGTCGCTGA Primer 2:CCAGCGGATTTTTTCGCTTTTTT
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 4% ^[10]
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 73% ^[10] Synonyms:zch-506::Tn10, zci-506::Tn10
edit table

See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

[back to top]

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10031
EchoBASE (EcoliWiki Page)	EchoBASE:EB0030
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1241
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C1705
EcoGene (EcoliWiki Page)	EcoGene:EG10031
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:ADHE
CGSC (EcoliWiki Page)	CGSC:1041
ASAP (EcoliWikiPage)	ASAP:ABE-0004164
edit table

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

[back to top]

Name	URL	Comments
edit table

See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

Categories

Product(s)

[back to top]

Nomenclature

Standard name	AdhE
Synonyms	fused acetaldehyde-CoA dehydrogenase^[1], iron-dependent alcohol dehydrogenase^[1], pyruvate-formate lyase deactivase^[1], B1241^[2]^[1], AdhC^[2]^[1], Ana^[2]^[1], AdhE^[2]^[1]
Product description	AdhE^[2]^[3]; Component of ; ADHE-CPLX^[2]
EC number (for enzymes)	1.2.1.10^[1] 1.1.1.1^[1]
edit table

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Notes

Function

[back to top]

Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0511	F	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0004022	alcohol dehydrogenase activity	GO_REF:0000002 GO_REF:0000003	IEA: Inferred from Electronic Annotation	InterPro:IPR012079 EC:1.1.1.1	F	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0046872	metal ion binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001670	F	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0005506	iron ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0006066	cellular alcohol metabolic process	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012079	P	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0008152	metabolic process	GO_REF:0000002 GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001670 InterPro:IPR015590	P	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0008774	acetaldehyde dehydrogenase (acetylating) activity	GO_REF:0000002 GO_REF:0000003	IEA: Inferred from Electronic Annotation	InterPro:IPR012079 EC:1.2.1.10	F	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0015976	carbon utilization	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012079	P	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0016491	oxidoreductase activity	GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR001670 InterPro:IPR015590 SP_KW:KW-0560	F	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0005515	protein binding	PMID:15690043^[12]	IPI: Inferred from Physical Interaction	UniProtKB:P0A7V0	F	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0005624	membrane fraction	PMID:16858726^[13]	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc 12.5 ^[11]	complete
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	required fields missing
	GO:0006113	fermentation				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0004022	alcohol dehydrogenase activity	PMID:6998946^[14]	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0008774	acetaldehyde dehydrogenase (acetylating) activity	PMID:6998946^[14]	IMP: Inferred from Mutant Phenotype		F		complete
edit table

Interactions

Partner Type	Partner	Notes	References
Protein	Subunits of ADHE-CPLX	could be indirect
edit table

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Notes

Localization

Compartment	Description	Evidence	Notes
edit table

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Notes

Structure and Physical Properties

[back to top]

Physical Properties

Sequence	at EcoCyc MAVTNVAELN ALVERVKKAQ REYASFTQEQ VDKIFRAAAL AAADARIPLA KMAVAESGMG IVEDKVIKNH FASEYIYNAY KDEKTCGVLS EDDTFGTITI AEPIGIICGI VPTTNPTSTA IFKSLISLKT RNAIIFSPHP RAKDATNKAA DIVLQAAIAA GAPKDLIGWI DQPSVELSNA LMHHPDINLI LATGGPGMVK AAYSSGKPAI GVGAGNTPVV IDETADIKRA VASVLMSKTF DNGVICASEQ SVVVVDSVYD AVRERFATHG GYLLQGKELK AVQDVILKNG ALNAAIVGQP AYKIAELAGF SVPENTKILI GEVTVVDESE PFAHEKLSPT LAMYRAKDFE DAVEKAEKLV AMGGIGHTSC LYTDQDNQPA RVSYFGQKMK TARILINTPA SQGGIGDLYN FKLAPSLTLG CGSWGGNSIS ENVGPKHLIN KKTVAKRAEN MLWHKLPKSI YFRRGSLPIA LDEVITDGHK RALIVTDRFL FNNGYADQIT SVLKAAGVET EVFFEVEADP TLSIVRKGAE LANSFKPDVI IALGGGSPMD AAKIMWVMYE HPETHFEELA LRFMDIRKRI YKFPKMGVKA KMIAVTTTSG TGSEVTPFAV VTDDATGQKY PLADYALTPD MAIVDANLVM DMPKSLCAFG GLDAVTHAME AYVSVLASEF SDGQALQALK LLKEYLPASY HEGSKNPVAR ERVHSAATIA GIAFANAFLG VCHSMAHKLG SQFHIPHGLA NALLICNVIR YNANDNPTKQ TAFSQYDRPQ ARRRYAEIAD HLGLSAPGDR TAAKIEKLLA WLETLKAELG IPKSIREAGV QEADFLANVD KLSEDAFDDQ CTGANPRYPL ISELKQILLD TYYGRDYVEG ETAAKKEAAP AKAEKKAKKS A
Mol. Wt	96.13 kDa (calc) ^[2]
pI	6.72^[2]
Extinction coefficient	69220 - 70345 (calc based on 28 Y, 5 W, and 9 C residues)
edit table

See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
edit table

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Structure

Structures	Representative homolog: 1O2D\|A (Thermotoga maritima) 41-242 of 891 residues (E-value: 1.3e-19) (Percent Identity: 31.34) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
edit table

Structures

Representative homolog:
1O2D|A (Thermotoga maritima)
41-242 of 891 residues (E-value: 1.3e-19) (Percent Identity: 31.34)
View all other structures.

Models

View models at:

edit table

See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Notes

Gene Product Resources

[back to top]

Resource type	Source	Notes/Reference
edit table

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Notes

Accessions in Other Databases

[back to top]

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:ADHE-MONOMER
UniProt (EcoliWiki Page)	UniProt:P0A9Q7
PFAM (EcoliWiki Page)	PFAM:PF00465
RefSeq (EcoliWiki Page)	RefSeq:NP_415757
ModBase (EcoliWiki Page)	ModBase:P0A9Q7
edit table

See Help:Product_accessions for help entering or editing information in this section of EcoliWiki.

Notes

Links

[back to top]

Name	URL	Comments
edit table

See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

Categories

[back to top]

Expression

[back to top]

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

[back to top]

Transcription unit(s)
adhE
edit table

Figure courtesy of RegulonDB

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

gbrowse_img.php?coord=1297324..1297364&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled+flip&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
edit table

See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b1241 (EcoliWiki Page)	Summary of data for adhE from multiple microarray studies
microarray	GEO Profiles:b1241 (EcoliWiki Page)	NCBI GEO profiles for adhE
edit table

See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Expression Resources

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1297274..1297865) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ04; Well:B3^[15]
edit table

See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Notes

Accessions Related to adhE Expression

[back to top]

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10031
EchoBASE (EcoliWiki Page)	EchoBASE:EB0030
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b1241
EcoGene (EcoliWiki Page)	EcoGene:EG10031
edit table

See Help:Expression_accessions for help entering or editing information in this section of EcoliWiki.

Notes

Links

[back to top]

Name	URL	Comments
edit table

See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

Categories

[back to top]

Evolution

[back to top]

Homologs in Other Organisms

[back to top]

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000018765 (score: 1.000; bootstrap: 100%) ENSAPMP00000023197 (score: 0.222) YP_026233 (score: 0.172)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000005874 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.144)	From Inparanoid:20070104
Caenorhabditis briggsae	YP_026233 (score: 0.255) WBGene00025759 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	YP_026233 (score: 0.250) WBGene00012608 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000010872 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.147)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000009349 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.153)	From Inparanoid:20070104
Danio rerio	YP_026233 (score: 0.216) ZDB-GENE-040426-2070 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-3017 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	YP_026233 (score: 1.000; bootstrap: 96%) DDB0219524 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	YP_026233 (score: 0.250) FBgn0017482 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	YP_026233 (score: 1.000; bootstrap: 87%) GA17444-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000276576 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.228)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000001187 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.205)	From Inparanoid:20070104
Mus musculus	MGI:1923437 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.225)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000034755 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.300)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000009462 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.228)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000181441 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.200)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00008613001 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.240)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000050261 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.177)	From Inparanoid:20070104
Shigella flexneri	ADHE	From SHIGELLACYC
E. coli O157	ADHE	From ECOO157CYC
edit table

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Do-It-Yourself Web Tools

Notes

Families

[back to top]

Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF00465
edit table

See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Notes

Links

[back to top]

Name	URL	Comments
edit table

See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ Clark D & Cronan JE Jr (1980) Escherichia coli mutants with altered control of alcohol dehydrogenase and nitrate reductase. J Bacteriol 141: 177-83 PubMed EcoliWiki page
↑ ^7.0 ^7.1 Lorowitz W & Clark D (1982) Escherichia coli mutants with a temperature-sensitive alcohol dehydrogenase. J Bacteriol 152: 935-8 PubMed EcoliWiki page
↑ ^8.0 ^8.1 Cunningham PR & Clark DP (1986) The use of suicide substrates to select mutants of Escherichia coli lacking enzymes of alcohol fermentation. Mol Gen Genet 205: 487-93 PubMed EcoliWiki page
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^10.0 ^10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^11.00 ^11.01 ^11.02 ^11.03 ^11.04 ^11.05 ^11.06 ^11.07 ^11.08 ^11.09 ^11.10 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
↑ Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed EcoliWiki page
↑ ^14.0 ^14.1 Clark DP & Cronan JE Jr (1980) Acetaldehyde coenzyme A dehydrogenase of Escherichia coli. J Bacteriol 144: 179-84 PubMed EcoliWiki page
↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

Categories

[back to top]

Retrieved from "http://ecoliwiki.net/colipedia/index.php/adhE:On_One_Page"

adhE:On One Page

From EcoliWiki

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to adhE Expression

Notes

Links

Categories

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Views

Personal tools

Navigation

Search

Toolbox