EcoliHub logo

Try the new EcoliHub and let us know what you'd like to see.

adhE:On One Page

From EcoliWiki

Jump to: navigation, search
Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]



See Help:Quickview for help with entering information in the Quickview table.

Standard Name

adhE

Gene Synonym(s)

ECK1235, b1241, JW1228, adhC, ana[1]

Product Desc.

AdhE[2][3];

Component of ADHE-CPLX[2]

Alcohol dehydrogenase, largely anaerobic; aerobic antioxidant; acetaldehyde-CoA dehydrogenase, CoA-linked; allyl alcohol resistance[4]

Product Synonyms(s)

fused acetaldehyde-CoA dehydrogenase[1], iron-dependent alcohol dehydrogenase[1], pyruvate-formate lyase deactivase[1], B1241[2][1], AdhC[2][1], Ana[2][1], AdhE[2][1]

Function from GO

Molecular Function

Cellular Component

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): adhE[2], OP00278

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(adhE)    Textpresso(adhE)

edit table

See Help:Quickview for help with entering information in the Quickview table.

Notes

[back to top]


The reported PFL deactivase activity of AdhE (Kessler, 1991, 1992) could not be reproduced (Nnyepi, 2007).[4]



Gene

[back to top]


Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

adhE

Mnemonic

Alcohol/acetaldehyde dehydrogenase

Synonyms

ECK1235, b1241, JW1228, adhC, ana[1]

edit table

Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

27.9 minutes 

MG1655: 1297344..1294669
gbrowse_img.php?coord=1294669..1297344&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1296914..1294239
gbrowse_img.php?coord=1294239..1296914&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1299698..1297023
gbrowse_img.php?coord=1297023..1299698&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1337585..1334910
gbrowse_img.php?coord=1334910..1337585&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 1184167..1186842
gbrowse_img.php?coord=1184167..1186842&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

edit table

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
edit table

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔadhE (Keio:JW1228)

deletion

deletion

PMID:16738554

Shigen
CGSC9113[5]

adhE

PMID:12519969

ASAP

At position 1923 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20272

ana-1

CGSC:5096

adhE80

PMID:6986356

CGSC:7028

adhE1

PMID:6752127

CGSC:7285

adhE16(ts)

temperature sensitive

CGSC:7289

adhE2

PMID:6752127

CGSC:7457

ana-2

adhE

CGSC:14693

adhE36

PMID:3550385

CGSC:92529

adhE38

PMID:3550385

CGSC:92530

ΔadhE748::kan

PMID:16738554

CGSC:99914


edit table

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW1228

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCTGTTACTAATGTCGCTGA

Primer 2:CCAGCGGATTTTTTCGCTTTTTT

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[5]

est. P1 cotransduction: 4% [6]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[5]

est. P1 cotransduction: 73% [6]
Synonyms:zch-506::Tn10, zci-506::Tn10

edit table

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10031

EchoBASE (EcoliWiki Page)

EchoBASE:EB0030

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1241

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1705

EcoGene (EcoliWiki Page)

EcoGene:EG10031

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ADHE

CGSC (EcoliWiki Page)

CGSC:1041

ASAP (EcoliWikiPage)

ASAP:ABE-0004164


edit table

Notes

Links

[back to top]



Product(s)

[back to top]



Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AdhE

Synonyms

fused acetaldehyde-CoA dehydrogenase[1], iron-dependent alcohol dehydrogenase[1], pyruvate-formate lyase deactivase[1], B1241[2][1], AdhC[2][1], Ana[2][1], AdhE[2][1]

Product description

AdhE[2][3];

Component of ADHE-CPLX[2]

Alcohol dehydrogenase, largely anaerobic; aerobic antioxidant; acetaldehyde-CoA dehydrogenase, CoA-linked; allyl alcohol resistance[4]

EC number (for enzymes)
edit table

Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0003824

catalytic activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0511

F

Seeded from EcoCyc [7]

complete

GO:0004022

alcohol dehydrogenase activity

GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR012079
EC:1.1.1.1

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0046872

metal ion binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001670

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0005506

iron ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc [7]

complete

GO:0006066

alcohol metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012079

P

Seeded from EcoCyc [7]

complete

GO:0008152

metabolic process

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001670
InterPro:IPR015590

P

Seeded from EcoCyc 12.5 [8]

complete

GO:0008774

acetaldehyde dehydrogenase (acetylating) activity

GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR012079
EC:1.2.1.10

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0015976

carbon utilization

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012079

P

Seeded from EcoCyc [7]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001670
InterPro:IPR015590
SP_KW:KW-0560

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0005515

protein binding

PMID:15690043

IPI: Inferred from Physical Interaction

UniProtKB:P0A7V0

F

Seeded from EcoCyc [7]

complete

GO:0005624

membrane fraction

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0005739

mitochondrion

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0055114

oxidation reduction

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001670
InterPro:IPR018211
SP_KW:KW-0560

P

Seeded from EcoCyc [7]

complete

GO:0006113

fermentation

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0004022

alcohol dehydrogenase activity

PMID:6998946

IMP: Inferred from Mutant Phenotype

F

complete

GO:0008774

acetaldehyde dehydrogenase (acetylating) activity

PMID:6998946

IMP: Inferred from Mutant Phenotype

F

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of ADHE-CPLX

could be indirect

Protein

nadE

PMID:16606699

Experiment(s):EBI-1139291

Protein

lacZ

PMID:16606699

Experiment(s):EBI-1139291

Protein

rpmB

PMID:16606699

Experiment(s):EBI-1139291

Protein

intR

PMID:16606699

Experiment(s):EBI-1139291

Protein

rplB

PMID:16606699

Experiment(s):EBI-1139291

Protein

rsuA

PMID:16606699

Experiment(s):EBI-1139291

Protein

rpsB

PMID:15690043

Experiment(s):EBI-891082

edit table

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
edit table

Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MAVTNVAELN ALVERVKKAQ REYASFTQEQ VDKIFRAAAL AAADARIPLA KMAVAESGMG
IVEDKVIKNH FASEYIYNAY KDEKTCGVLS EDDTFGTITI AEPIGIICGI VPTTNPTSTA
IFKSLISLKT RNAIIFSPHP RAKDATNKAA DIVLQAAIAA GAPKDLIGWI DQPSVELSNA
LMHHPDINLI LATGGPGMVK AAYSSGKPAI GVGAGNTPVV IDETADIKRA VASVLMSKTF
DNGVICASEQ SVVVVDSVYD AVRERFATHG GYLLQGKELK AVQDVILKNG ALNAAIVGQP
AYKIAELAGF SVPENTKILI GEVTVVDESE PFAHEKLSPT LAMYRAKDFE DAVEKAEKLV
AMGGIGHTSC LYTDQDNQPA RVSYFGQKMK TARILINTPA SQGGIGDLYN FKLAPSLTLG
CGSWGGNSIS ENVGPKHLIN KKTVAKRAEN MLWHKLPKSI YFRRGSLPIA LDEVITDGHK
RALIVTDRFL FNNGYADQIT SVLKAAGVET EVFFEVEADP TLSIVRKGAE LANSFKPDVI
IALGGGSPMD AAKIMWVMYE HPETHFEELA LRFMDIRKRI YKFPKMGVKA KMIAVTTTSG
TGSEVTPFAV VTDDATGQKY PLADYALTPD MAIVDANLVM DMPKSLCAFG GLDAVTHAME
AYVSVLASEF SDGQALQALK LLKEYLPASY HEGSKNPVAR ERVHSAATIA GIAFANAFLG
VCHSMAHKLG SQFHIPHGLA NALLICNVIR YNANDNPTKQ TAFSQYDRPQ ARRRYAEIAD
HLGLSAPGDR TAAKIEKLLA WLETLKAELG IPKSIREAGV QEADFLANVD KLSEDAFDDQ
CTGANPRYPL ISELKQILLD TYYGRDYVEG ETAAKKEAAP AKAEKKAKKS A
Length

891

Mol. Wt

96.129 kDa

pI

6.8 (calculated)

Extinction coefficient

69,220 - 70,345 (calc based on 28 Y, 5 W, and 9 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed

UniProt:P0A9Q7


edit table


Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:ADHE-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0A9Q7

PFAM (EcoliWiki Page)

PFAM:PF00465

RefSeq (EcoliWiki Page)

RefSeq:NP_415757


edit table

Notes

Links

[back to top]




Expression

[back to top]



Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]


See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

adhE

edit table
Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


gbrowse_img.php?coord=1297324..1297364&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
edit table


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b1241 (EcoliWiki Page)

NCBI GEO profiles for adhE

microarray

GenExpDB:b1241 (EcoliWiki Page)

Summary of data for adhE from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (1297274..1297865) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ04; Well:B3[9]

edit table

Notes

Accessions Related to adhE Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10031

EchoBASE (EcoliWiki Page)

EchoBASE:EB0030

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1241

EcoGene (EcoliWiki Page)

EcoGene:EG10031


edit table

Notes

Links

[back to top]



Evolution

[back to top]



Homologs in Other Organisms

[back to top]


See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Apis mellifera

  • ENSAPMP00000018765 (score: 1.000; bootstrap: 100%)
  • ENSAPMP00000023197 (score: 0.222)
  • YP_026233 (score: 0.172)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000005874 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.144)

From Inparanoid:20070104

Caenorhabditis briggsae

  • YP_026233 (score: 0.255)
  • WBGene00025759 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • YP_026233 (score: 0.250)
  • WBGene00012608 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000010872 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.147)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000009349 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.153)

From Inparanoid:20070104

Danio rerio

  • YP_026233 (score: 0.216)
  • ZDB-GENE-040426-2070 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040425-3017 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • YP_026233 (score: 1.000; bootstrap: 96%)
  • DDB0219524 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • YP_026233 (score: 0.250)
  • FBgn0017482 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • YP_026233 (score: 1.000; bootstrap: 87%)
  • GA17444-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000276576 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.228)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000001187 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.205)

From Inparanoid:20070104

Mus musculus

  • MGI:1923437 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.225)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000034755 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.300)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000009462 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.228)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000181441 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.200)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00008613001 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.240)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000050261 (score: 1.000; bootstrap: 100%)
  • YP_026233 (score: 0.177)

From Inparanoid:20070104

Shigella flexneri

ADHE

From SHIGELLACYC

E. coli O157

ADHE

From ECOO157CYC

edit table


Do-It-Yourself Web Tools

Notes

Families

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00465


edit table

Notes

Links

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ 8.0 8.1 8.2 8.3 8.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

Categories

[back to top]


Retrieved from "http://ecoliwiki.net/colipedia/index.php/adhE:On_One_Page"
Personal tools