adk:Gene - EcoliWiki
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adk:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

adk

Mnemonic
Synonyms

ECK0468, b0474, JW0463, dnaW, plsA[1]

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Notes

dnaW and adk were shown to be allelic by Henson an Walker[2].

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

10.7 minutes 

MG1655: 496399..497043
gbrowse_img.php?coord=495399..498043&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 496399..497043
gbrowse_img.php?coord=495399..498043&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

adk-15(ts)

temperature sensitive

CGSC:11623

adk-2(ts)

temperature sensitive

CGSC:11620

adk-31(ts)

temperature sensitive

CGSC:11625

adk-3(ts)

temperature sensitive

CGSC:11738

adk-9(ts)

temperature sensitive

CGSC:11756


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW0463

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCCGTATCATTCTGCTTGGCGC

Primer 2:CCGCCGAGGATTTTTTCCAGATC

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[4]

est. P1 cotransduction: 22% [5]

Synonyms:zba-3054::Tn10

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[4]

est. P1 cotransduction: 86% [5]

Synonyms:zbb-30-55::TnlO, zba-3055::Tn10

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10032

EchoBASE (EcoliWiki Page)

EchoBASE:EB0031

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0474

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0594

EcoGene (EcoliWiki Page)

EcoGene:EG10032

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ADK

CGSC (EcoliWiki Page)

CGSC:1040

ASAP (EcoliWikiPage)

ASAP:ABE-0001645


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Henson JM et al. (1982) The Escherichia coli dnaW mutation is an allele of the adk gene. Mol Gen Genet 186: 488-92 PubMed EcoliWiki page
  3. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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