afuC:Gene - EcoliWiki
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afuC:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

afuC

Mnemonic
Synonyms

ECK0264, b0262, JW0254, yagC, fbpC[1][2]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

5.97 minutes 

MG1655: 278026..276980
gbrowse_img.php?coord=275980..279026&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 278026..276980
gbrowse_img.php?coord=275980..279026&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔafuC (Keio:JW0254)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8478[4]

ΔafuC-779::kan

PMID:16738554[3]

CGSC:99932


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW0254

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCACTCAGAAAAATTTCGTTGA

Primer 2:CCTGCCGCATCCGCCAGAACAAA

proA81::Tn10

Linked marker

CAG18447 = CGSC7330[4]

est. P1 cotransduction: 54% [6]

lacI3042::Tn10

Linked marker

CAG18439 = CGSC7334[4]

est. P1 cotransduction: % [6]

Synonyms:lacI42::Tn10


CAG18439 also carries lacZ118(0c) (CGSC).

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12340

EchoBASE (EcoliWiki Page)

EchoBASE:EB2244

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0262

EcoGene (EcoliWiki Page)

EcoGene:EG12340

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:AFUC

ASAP (EcoliWikiPage)

ASAP:ABE-0000898


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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