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Gene Name

ahpC (synonyms: ECK0599, b0605, JW0598, ssi8)[1][2]

Product(s)

AhpC product[1]: alkyl hydroperoxide reductase, C22 subunit product, B0605 product, Ssi8 product

Description

AhpC component[2][3]

Component of alkylhydroperoxide reductase[3]

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ahpCF[2], OP00101, ahpC

Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name

ahpC

Mnemonic
Synonyms

ECK0599, b0605, JW0598, ssi8[1][2]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

13.75 minutes 

MG1655: 638168..638731
gbrowse_img.php?coord=637168..639731&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 638168..638731
gbrowse_img.php?coord=637168..639731&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔahpC (Keio:JW0598)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC8713[5]

ahpC*

triplet repeat expansion

Other

PMID:18206967[6]

ΔahpC744::kan

PMID:16738554[4]

CGSC:99954


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW0598

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCTCCTTGATTAACACCAAAAT

Primer 2:CCGATTTTACCAACCAGGTCCAG

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[5]

est. P1 cotransduction: 92% [8]

Synonyms:zbd-601::Tn10, zbe-601::Tn10

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[5]

est. P1 cotransduction: 57% [8]

Synonyms:zbe-280::Tn10

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11384

EchoBASE (EcoliWiki Page)

EchoBASE:EB1357

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0605

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0694

EcoGene (EcoliWiki Page)

EcoGene:EG11384

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:AHPC

CGSC (EcoliWiki Page)

CGSC:31190

ASAP (EcoliWikiPage)

ASAP:ABE-0002090


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Notes

Links

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NameURLComments
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Categories

Product(s)

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Nomenclature

Standard name

AhpC

Synonyms

alkyl hydroperoxide reductase, C22 subunit[1], B0605[2][1], AhpC[2][1], Ssi8[2][1]

Product description

AhpC component[2][3];

Component of ; alkylhydroperoxide reductase[3]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0004601

peroxidase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0575

F

Seeded from EcoCyc 12.5 [9]

complete

GO:0016209

antioxidant activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000866
SP_KW:KW-0049

F

Seeded from EcoCyc 12.5 [9]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000866
SP_KW:KW-0560

F

Seeded from EcoCyc 12.5 [9]

complete

GO:0051920

peroxiredoxin activity

GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.11.1.15

F

Seeded from EcoCyc 12.5 [9]

complete

Under review

GO:0005624

membrane fraction

PMID:16858726[10]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc 12.5 [9]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required field missing

GO:0006805

xenobiotic metabolic process

PMID:7644465[11]

IMP: Inferred from Mutant Phenotype

P

tetralin, cyclohexane and proplybenze catabolism.

complete

GO:0004601

peroxidase activity

PMID:11717276[12]

IGI: Inferred from Genetic Interaction

EcoliWiki:katG

F

complete

GO:0051920

peroxiredoxin activity

PMID:7644465[11]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0005737

cytoplasm

PMID:7499381[13]

IDA: Inferred from Direct Assay

C

complete

GO:0016209

antioxidant activity

PMID:7644465[11]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0016684

oxidoreductase activity, acting on peroxide as acceptor

PMID:7644465[11]

IMP: Inferred from Mutant Phenotype

F

complete

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Interactions

Partner TypePartnerNotesReferences

Protein

Subunits of alkylhydroperoxide reductase

could be indirect


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Notes

Localization

CompartmentDescriptionEvidenceNotes

cytoplasm


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Notes

Structure and Physical Properties

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Physical Properties

Sequence

at EcoCyc

MSLINTKIKP FKNQAFKNGE FIEITEKDTE GRWSVFFFYP ADFTFVCPTE LGDVADHYEE
LQKLGVDVYA VSTDTHFTHK AWHSSSETIA KIKYAMIGDP TGALTRNFDN MREDEGLADR
ATFVVDPQGI IQAIEVTAEG IGRDASDLLR KIKAAQYVAS HPGEVCPAKW KEGEATLAPS
LDLVGKI
Mol. Wt

20.76 kDa (calc) [2]

pI
Extinction coefficient

23950 - 24200 (calc based on 5 Y, 3 W, and 2 C residues)

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Domains/Motifs/Modification Sites

TypeResiduesDescriptionNotes

Domain

2 - 157

Thioredoxin

UniProt:P0AE08

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Structure

Structures
  • Representative homolog:
    2C0D|A (Plasmodium falciparum)
    24-208 of 187 residues (E-value: 1.2e-22) (Percent Identity: 28.88)
  • View all other structures.
Models

View models at:

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Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11384-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P0AE08

UniProt (EcoliWiki Page)

UniProt:P0AE08

PFAM (EcoliWiki Page)

PFAM:PF00578

RefSeq (EcoliWiki Page)

RefSeq:NP_415138

ModBase (EcoliWiki Page)

ModBase:P0AE08

PDB (EcoliWiki Page)

PDB:1KYG


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Notes

Links

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NameURLComments
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Categories

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)

ahpCF

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Figure courtesy of RegulonDB
Figure courtesy of RegulonDB

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=638148..638188&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele NameMutationPhenotypeReference
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Expression Studies

TypeReferenceNotes

microarray

GenExpDB:b0605 (EcoliWiki Page)

Summary of data for ahpC from multiple microarray studies

microarray

GEO Profiles:b0605 (EcoliWiki Page)

NCBI GEO profiles for ahpC

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Expression Resources

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (637759..638206) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ13; Well:D11[14]

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Notes

Accessions Related to ahpC Expression

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11384

EchoBASE (EcoliWiki Page)

EchoBASE:EB1357

EcoliGenExpDB (EcoliWiki Page)

GenExpDB:b0605

EcoGene (EcoliWiki Page)

EcoGene:EG11384

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Notes

Links

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NameURLComments
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Categories

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Evolution

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Homologs in Other Organisms

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OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000026815 (score: 1.000; bootstrap: 98%)
  • ENSANGP00000010951 (score: 1.000; bootstrap: 99%)
  • ENSANGP00000019782 (score: 0.502)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000033015 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G06290 (score: 1.000; bootstrap: 99%)
  • AT3G11630 (score: 0.777)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000011505 (score: 1.000; bootstrap: 99%)
  • ENSBTAP00000015996 (score: 0.366)
  • ENSBTAP00000008107 (score: 0.348)
  • ENSBTAP00000004751 (score: 0.242)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00025442 (score: 1.000; bootstrap: 99%)
  • WBGene00037564 (score: 0.114)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00006434 (score: 1.000; bootstrap: 99%)
  • WBGene00011110 (score: 0.447)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000025278 (score: 1.000; bootstrap: 99%)
  • ENSCAFP00000006827 (score: 0.668)
  • ENSCAFP00000003384 (score: 0.592)
  • ENSCAFP00000019709 (score: 0.513)
  • ENSCAFP00000017833 (score: 0.453)
  • ENSCAFP00000007440 (score: 0.415)
  • ENSCAFP00000008263 (score: 0.351)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000007255 (score: 1.000; bootstrap: 99%)
  • ENSCINP00000013246 (score: 0.367)
  • ENSCINP00000006141 (score: 0.338)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-040718-170 (score: 1.000; bootstrap: 99%)
  • ZDB-CDNA-040718-193 (score: 1.000; bootstrap: 99%)
  • ZDB-CDNA-050320-35 (score: 0.729)
  • ZDB-GENE-050320-35 (score: 0.729)
  • ZDB-CDNA-050320-21 (score: 0.469)
  • ZDB-CDNA-050417-74 (score: 0.465)
  • ZDB-GENE-030826-18 (score: 0.465)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0231647 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0038519 (score: 1.000; bootstrap: 96%)
  • FBgn0040308 (score: 0.350)
  • FBgn0040309 (score: 0.297)
  • FBgn0036490 (score: 0.209)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA19159-PA (score: 1.000; bootstrap: 99%)
  • GA11781-PA (score: 0.305)
  • GA14060-PA (score: 0.284)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000015246 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000026337 (score: 0.351)
  • ENSGALP00000016629 (score: 0.332)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000298510 (score: 1.000; bootstrap: 99%)
  • ENSP00000319964 (score: 0.357)
  • ENSP00000301522 (score: 0.336)
  • ENSP00000262746 (score: 0.328)
  • ENSP00000338995 (score: 0.056)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000010873 (score: 1.000; bootstrap: 99%)
  • ENSMMUP00000028144 (score: 0.354)
  • ENSMMUP00000007512 (score: 0.244)
  • ENSMMUP00000008176 (score: 0.239)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000008649 (score: 1.000; bootstrap: 99%)
  • ENSMODP00000002901 (score: 0.637)
  • ENSMODP00000009688 (score: 0.548)
  • ENSMODP00000011601 (score: 0.489)

From Inparanoid:20070104

Mus musculus

  • MGI:109486 (score: 1.000; bootstrap: 99%)
  • MGI:99523 (score: 0.668)
  • MGI:3645884 (score: 0.629)
  • MGI:1859815 (score: 0.529)
  • MGI:88034 (score: 0.467)

From Inparanoid:20070104

Oryza gramene

  • O81480 (score: 1.000; bootstrap: 99%)
  • Q96468 (score: 0.723)
  • P80602 (score: 0.720)
  • Q6ER94 (score: 0.696)
  • Q7X679 (score: 0.258)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000001167 (score: 1.000; bootstrap: 99%)
  • ENSPTRP00000037272 (score: 0.335)
  • ENSPTRP00000017998 (score: 0.248)
  • ENSPTRP00000004125 (score: 0.198)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000004799 (score: 1.000; bootstrap: 99%)
  • ENSRNOP00000023132 (score: 0.688)
  • ENSRNOP00000023972 (score: 0.677)
  • ENSRNOP00000043982 (score: 0.502)
  • ENSRNOP00000015186 (score: 0.460)
  • ENSRNOP00000029235 (score: 0.266)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YDR453C (score: 1.000; bootstrap: 90%)
  • YML028W (score: 0.865)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCC5763c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000135519 (score: 1.000; bootstrap: 99%)
  • NEWSINFRUP00000151126 (score: 0.714)
  • NEWSINFRUP00000159443 (score: 0.530)
  • NEWSINFRUP00000139714 (score: 0.440)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00013358001 (score: 1.000; bootstrap: 99%)
  • GSTENP00022646001 (score: 0.717)
  • GSTENP00019030001 (score: 0.517)
  • GSTENP00037525001 (score: 0.212)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000024196 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Shigella flexneri

AHPC

From SHIGELLACYC

E. coli O157

AHPC

From ECOO157CYC

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Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00578

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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Yamamoto Y et al. (2008) Mutant AhpC peroxiredoxins suppress thiol-disulfide redox deficiencies and acquire deglutathionylating activity. Mol Cell 29: 36-45 PubMed EcoliWiki page
  7. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  9. 9.0 9.1 9.2 9.3 9.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  10. Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed EcoliWiki page
  11. 11.0 11.1 11.2 11.3 Ferrante AA et al. (1995) Cloning of an organic solvent-resistance gene in Escherichia coli: the unexpected role of alkylhydroperoxide reductase. Proc Natl Acad Sci U S A 92: 7617-21 PubMed EcoliWiki page
  12. Seaver LC & Imlay JA (2001) Alkyl hydroperoxide reductase is the primary scavenger of endogenous hydrogen peroxide in Escherichia coli. J Bacteriol 183: 7173-81 PubMed EcoliWiki page
  13. Cha MK et al. (1995) Thioredoxin-linked "thiol peroxidase" from periplasmic space of Escherichia coli. J Biol Chem 270: 28635-41 PubMed EcoliWiki page
  14. Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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