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ahpC:On One Page

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Standard Name

ahpC

Gene Synonym(s)

ECK0599, b0605, JW0598, ssi8[1][2]

Product Desc.

AhpC component[2][3];

Component of alkylhydroperoxide reductase[3]

Alkyl hydroperoxide reductase, subunit C; reduced by the AhpF subunit[4]

Product Synonyms(s)

alkyl hydroperoxide reductase, C22 subunit[1], B0605[2][1], AhpC[2][1], Ssi8[2][1]

Function from GO

Cellular Component

Molecular Function

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ahpCF[2], OP00101, ahpC

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(ahpC)    Textpresso(ahpC)

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Notes

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AhpCF protects aerobic, phosphate-starved cells from oxidative damage. Binds TrxA (Kumar, 2004).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ahpC

Mnemonic

Alkyl hydroperoxide

Synonyms

ECK0599, b0605, JW0598, ssi8[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

13.75 minutes 

MG1655: 638168..638731
gbrowse_img.php?coord=638168..638731&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 620996..621559
gbrowse_img.php?coord=620996..621559&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 638168..638731
gbrowse_img.php?coord=638168..638731&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 577500..578063
gbrowse_img.php?coord=577500..578063&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

DH10B

DH10B: 690760..691323
gbrowse_img.php?coord=690760..691323&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 540928..541491
gbrowse_img.php?coord=540928..541491&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔahpC (Keio:JW0598)

deletion

deletion

PMID:16738554

Shigen
CGSC8713[5]

ahpC*

triplet repeat expansion

Other

PMID: 18206967

ΔahpC744::kan

PMID:16738554

CGSC:99954


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0598

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCCTTGATTAACACCAAAAT

Primer 2:CCGATTTTACCAACCAGGTCCAG

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[5]

est. P1 cotransduction: 92% [6]
Synonyms:zbd-601::Tn10, zbe-601::Tn10

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[5]

est. P1 cotransduction: 57% [6]
Synonyms:zbe-280::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11384

EchoBASE (EcoliWiki Page)

EchoBASE:EB1357

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0605

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0694

EcoGene (EcoliWiki Page)

EcoGene:EG11384

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:AHPC

CGSC (EcoliWiki Page)

CGSC:31190

ASAP (EcoliWikiPage)

ASAP:ABE-0002090


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AhpC

Synonyms

alkyl hydroperoxide reductase, C22 subunit[1], B0605[2][1], AhpC[2][1], Ssi8[2][1]

Product description

AhpC component[2][3];

Component of alkylhydroperoxide reductase[3]

Alkyl hydroperoxide reductase, subunit C; reduced by the AhpF subunit[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0005737

cytoplasm

PMID:7499381

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0004601

peroxidase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0575

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0016209

antioxidant activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000866
SP_KW:KW-0049

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000866
SP_KW:KW-0560

F

Seeded from EcoCyc [7]

complete

GO:0051920

peroxiredoxin activity

GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.11.1.15

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0005624

membrane fraction

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required field missing

GO:0005739

mitochondrion

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0045454

cell redox homeostasis

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017936

P

Seeded from EcoCyc [7]

complete

GO:0055114

oxidation reduction

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR017559
SP_KW:KW-0560

P

Seeded from EcoCyc [7]

complete

GO:0004601

peroxidase activity

PMID:11717276

IGI: Inferred from Genetic Interaction

EcoliWiki:katG

F

complete

GO:0005737

cytoplasm

PMID:7499381

IDA: Inferred from Direct Assay

C

complete

GO:0016209

antioxidant activity

PMID:7644465

IMP: Inferred from Mutant Phenotype

F

complete

GO:0016684

oxidoreductase activity, acting on peroxide as acceptor

PMID:7644465

IMP: Inferred from Mutant Phenotype

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of alkylhydroperoxide reductase

could be indirect

Protein

rhsC

PMID:16606699

Experiment(s):EBI-1137372

Protein

lacZ

PMID:16606699

Experiment(s):EBI-1137372

Protein

rhsE

PMID:16606699

Experiment(s):EBI-1137372

Protein

rtcB

PMID:16606699

Experiment(s):EBI-1137372

Protein

recB

PMID:16606699

Experiment(s):EBI-1137372

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

cytoplasm


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MSLINTKIKP FKNQAFKNGE FIEITEKDTE GRWSVFFFYP ADFTFVCPTE LGDVADHYEE
LQKLGVDVYA VSTDTHFTHK AWHSSSETIA KIKYAMIGDP TGALTRNFDN MREDEGLADR
ATFVVDPQGI IQAIEVTAEG IGRDASDLLR KIKAAQYVAS HPGEVCPAKW KEGEATLAPS
LDLVGKI
Length

187

Mol. Wt

20.761 kDa

pI

5.0 (calculated)

Extinction coefficient

23,950 - 24,200 (calc based on 5 Y, 3 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed

UniProt:P0AE08

Modification Site

84

phosphorylation site at S84

probability greater than 75%

PMID:17938405

Modification Site

146

phosphorylation site at S146

probability greater than 75%

PMID:17938405

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11384-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P0AE08

UniProt (EcoliWiki Page)

UniProt:P0AE08

PFAM (EcoliWiki Page)

PFAM:PF00578

RefSeq (EcoliWiki Page)

RefSeq:NP_415138


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ahpCF

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=638148..638188&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0605 (EcoliWiki Page)

NCBI GEO profiles for ahpC

microarray

GenExpDB:b0605 (EcoliWiki Page)

Summary of data for ahpC from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (637759..638206) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ13; Well:D11[9]

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Notes

Accessions Related to ahpC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11384

EchoBASE (EcoliWiki Page)

EchoBASE:EB1357

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0605

EcoGene (EcoliWiki Page)

EcoGene:EG11384


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000026815 (score: 1.000; bootstrap: 98%)
  • ENSANGP00000010951 (score: 1.000; bootstrap: 99%)
  • ENSANGP00000019782 (score: 0.502)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000033015 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G06290 (score: 1.000; bootstrap: 99%)
  • AT3G11630 (score: 0.777)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000011505 (score: 1.000; bootstrap: 99%)
  • ENSBTAP00000015996 (score: 0.366)
  • ENSBTAP00000008107 (score: 0.348)
  • ENSBTAP00000004751 (score: 0.242)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00025442 (score: 1.000; bootstrap: 99%)
  • WBGene00037564 (score: 0.114)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00006434 (score: 1.000; bootstrap: 99%)
  • WBGene00011110 (score: 0.447)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000025278 (score: 1.000; bootstrap: 99%)
  • ENSCAFP00000006827 (score: 0.668)
  • ENSCAFP00000003384 (score: 0.592)
  • ENSCAFP00000019709 (score: 0.513)
  • ENSCAFP00000017833 (score: 0.453)
  • ENSCAFP00000007440 (score: 0.415)
  • ENSCAFP00000008263 (score: 0.351)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000007255 (score: 1.000; bootstrap: 99%)
  • ENSCINP00000013246 (score: 0.367)
  • ENSCINP00000006141 (score: 0.338)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-040718-170 (score: 1.000; bootstrap: 99%)
  • ZDB-CDNA-040718-193 (score: 1.000; bootstrap: 99%)
  • ZDB-CDNA-050320-35 (score: 0.729)
  • ZDB-GENE-050320-35 (score: 0.729)
  • ZDB-CDNA-050320-21 (score: 0.469)
  • ZDB-CDNA-050417-74 (score: 0.465)
  • ZDB-GENE-030826-18 (score: 0.465)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0231647 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0038519 (score: 1.000; bootstrap: 96%)
  • FBgn0040308 (score: 0.350)
  • FBgn0040309 (score: 0.297)
  • FBgn0036490 (score: 0.209)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA19159-PA (score: 1.000; bootstrap: 99%)
  • GA11781-PA (score: 0.305)
  • GA14060-PA (score: 0.284)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000015246 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000026337 (score: 0.351)
  • ENSGALP00000016629 (score: 0.332)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000298510 (score: 1.000; bootstrap: 99%)
  • ENSP00000319964 (score: 0.357)
  • ENSP00000301522 (score: 0.336)
  • ENSP00000262746 (score: 0.328)
  • ENSP00000338995 (score: 0.056)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000010873 (score: 1.000; bootstrap: 99%)
  • ENSMMUP00000028144 (score: 0.354)
  • ENSMMUP00000007512 (score: 0.244)
  • ENSMMUP00000008176 (score: 0.239)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000008649 (score: 1.000; bootstrap: 99%)
  • ENSMODP00000002901 (score: 0.637)
  • ENSMODP00000009688 (score: 0.548)
  • ENSMODP00000011601 (score: 0.489)

From Inparanoid:20070104

Mus musculus

  • MGI:109486 (score: 1.000; bootstrap: 99%)
  • MGI:99523 (score: 0.668)
  • MGI:3645884 (score: 0.629)
  • MGI:1859815 (score: 0.529)
  • MGI:88034 (score: 0.467)

From Inparanoid:20070104

Oryza gramene

  • O81480 (score: 1.000; bootstrap: 99%)
  • Q96468 (score: 0.723)
  • P80602 (score: 0.720)
  • Q6ER94 (score: 0.696)
  • Q7X679 (score: 0.258)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000001167 (score: 1.000; bootstrap: 99%)
  • ENSPTRP00000037272 (score: 0.335)
  • ENSPTRP00000017998 (score: 0.248)
  • ENSPTRP00000004125 (score: 0.198)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000004799 (score: 1.000; bootstrap: 99%)
  • ENSRNOP00000023132 (score: 0.688)
  • ENSRNOP00000023972 (score: 0.677)
  • ENSRNOP00000043982 (score: 0.502)
  • ENSRNOP00000015186 (score: 0.460)
  • ENSRNOP00000029235 (score: 0.266)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YDR453C (score: 1.000; bootstrap: 90%)
  • YML028W (score: 0.865)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCC5763c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000135519 (score: 1.000; bootstrap: 99%)
  • NEWSINFRUP00000151126 (score: 0.714)
  • NEWSINFRUP00000159443 (score: 0.530)
  • NEWSINFRUP00000139714 (score: 0.440)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00013358001 (score: 1.000; bootstrap: 99%)
  • GSTENP00022646001 (score: 0.717)
  • GSTENP00019030001 (score: 0.517)
  • GSTENP00037525001 (score: 0.212)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000024196 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Shigella flexneri

AHPC

From SHIGELLACYC

E. coli O157

AHPC

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00578


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ 8.0 8.1 8.2 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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