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Quickview | Gene | Product(s) | Expression| Evolution | References |

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Gene Name

ais (synonyms: ECK2245, b2252, JW2246)[1]

Product(s)

Ais product[1]: conserved protein product, B2252 product

Description

conserved protein[2] protein induced by aluminum[3]

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ais[2]

Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name

ais

Mnemonic
Synonyms

ECK2245, b2252, JW2246[1]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

50.93 minutes 

MG1655: 2363642..2363040
gbrowse_img.php?coord=2362040..2364642&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2370290..2369688
gbrowse_img.php?coord=2368688..2371290&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

Δais (Keio:JW2246)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC9811[5]

Δais-733::kan

PMID:16738554[4]

CGSC:99958


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW2246

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTTAGCTTTTTGCCGCTCTTC

Primer 2:CCGTGGTTAACGAATTCCCCATC

zfa-723::Tn10

Linked marker

CAG12178 gyrA+ = CGSC7526[5]

est. P1 cotransduction: 48% [7]

Synonyms:zei-723::Tn10, zfa-723::Tn10


The original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[5]

est. P1 cotransduction: 37% [7]

Synonyms:zej-223::Tn10

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7165

EchoBASE (EcoliWiki Page)

EchoBASE:EB2949

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2252

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2794

EcoGene (EcoliWiki Page)

EcoGene:EG13155

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:AIS

ASAP (EcoliWikiPage)

ASAP:ABE-0007451


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Notes

Links

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NameURLComments
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Categories

Product(s)

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Nomenclature

Standard name

Ais

Synonyms

conserved protein[1], B2252[2][1], Ais[2][1]

Product description

conserved protein[2]; protein induced by aluminum[3]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0016020

membrane

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0016021

integral to membrane

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc 12.5 [8]

complete

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Interactions

Partner TypePartnerNotesReferences
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Notes

Localization

CompartmentDescriptionEvidenceNotes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence

at EcoCyc 

MLAFCRSSLK SKKYIIILLA LAAIAGLGTH AAWSSNGLPR IDNKTLARLA QQHPVVVLFR
HAERCDRSTN QCLSDKTGIT VKGTQDAREL GNAFSADIPD FDLYSSNTVR TIQSATWFSA
GKKLTVDKRL LQCGNEIYSA IKDLQSKAPD KNIVIFTHNH CLTYIAKDKR DATFKPDYLD
GLVMHVEKGK VYLDGEFVNH
Mol. Wt

22.26 kDa (calc) [2]

pI
Extinction coefficient

19940 - 20565 (calc based on 6 Y, 2 W, and 5 C residues)

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Domains/Motifs/Modification Sites

TypeResiduesDescriptionNotes
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Structure

Structures
  • No results found.
Models

View models at:

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Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7165-MONOMER

UniProt (EcoliWiki Page)

UniProt:P45565

RefSeq (EcoliWiki Page)

RefSeq:NP_416755

ModBase (EcoliWiki Page)

ModBase:P45565


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Notes

Links

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NameURLComments
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Categories

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)

ais

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Figure courtesy of RegulonDB
Figure courtesy of RegulonDB

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2363622..2363662&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled+flip&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele NameMutationPhenotypeReference
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Expression Studies

TypeReferenceNotes

microarray

GenExpDB:b2252 (EcoliWiki Page)

Summary of data for ais from multiple microarray studies

microarray

GEO Profiles:b2252 (EcoliWiki Page)

NCBI GEO profiles for ais

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Expression Resources

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (2363553..2363958) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ12; Well:A4[9]

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Notes

Accessions Related to ais Expression

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7165

EchoBASE (EcoliWiki Page)

EchoBASE:EB2949

EcoliGenExpDB (EcoliWiki Page)

GenExpDB:b2252

EcoGene (EcoliWiki Page)

EcoGene:EG13155

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Notes

Links

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NameURLComments
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Categories

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Evolution

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Homologs in Other Organisms

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OrganismHomologs (Statistics)Comments

Shigella flexneri

AIS

From SHIGELLACYC

E. coli O157

AIS

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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OrganismHomologs (Statistics)Comments

Shigella flexneri

AIS

From SHIGELLACYC

E. coli O157

AIS

From ECOO157CYC

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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  8. ↑ 8.0 8.1 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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