E. coli is indeed the coolest thing on the planet - Carl Zimmer
alaS:Gene Product(s)
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
| Standard name |
AlaS |
|---|---|
| Synonyms |
alanyl-tRNA synthetase[1], Act[2][1], B2697[2][1], LovB[2][1], AlaS[2][1], ala-act[2][1] |
| Product description |
alanyl-tRNA synthetase[2]; AlaS transcriptional repressor; alanyl-tRNA synthetase[3]; Component of ; alanyl-tRNA synthetase[2]; AlaS transcriptional repressor; alanyl-tRNA synthetase[3] |
| EC number (for enzymes) | |
| edit table |
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005524 |
ATP binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0004813 |
alanine-tRNA ligase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0004812 |
aminoacyl-tRNA ligase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0003676 |
nucleic acid binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0046872 |
metal ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0006412 |
translation |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0006419 |
alanyl-tRNA aminoacylation |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0008270 |
zinc ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0016874 |
ligase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0004813 |
alanine-tRNA ligase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0006419 |
alanyl-tRNA aminoacylation |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0008270 |
zinc ion binding |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0016566 |
specific transcriptional repressor activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0045892 |
negative regulation of transcription, DNA-dependent |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0006418 |
tRNA aminoacylation for protein translation |
P |
Seeded from Riley et al 2006 [1]. |
required fields missing | ||||
| GO:0045892 |
negative regulation of transcription, DNA-dependent |
IDA |
P |
Seeded from EcoCyc 11.1[3]. |
required fields missing | |||
| edit table |
Interactions
| Partner Type | Partner | Notes | References |
|---|---|---|---|
|
Protein |
Subunits of alanyl-tRNA synthetase |
could be indirect |
|
| edit table |
See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Notes
Localization
| Compartment | Description | Evidence | Notes |
|---|---|---|---|
| edit table |
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Notes
Structure and Physical Properties
Physical Properties
| Sequence |
MSKSTAEIRQ AFLDFFHSKG HQVVASSSLV PHNDPTLLFT NAGMNQFKDV FLGLDKRNYS RATTSQRCVR AGGKHNDLEN VGYTARHHTF FEMLGNFSFG DYFKHDAIQF AWELLTSEKW FALPKERLWV TVYESDDEAY EIWEKEVGIP RERIIRIGDN KGAPYASDNF WQMGDTGPCG PCTEIFYDHG DHIWGGPPGS PEEDGDRYIE IWNIVFMQFN RQADGTMEPL PKPSVDTGMG LERIAAVLQH VNSNYDIDLF RTLIQAVAKV TGATDLSNKS LRVIADHIRS CAFLIADGVM PSNENRGYVL RRIIRRAVRH GNMLGAKETF FYKLVGPLID VMGSAGEDLK RQQAQVEQVL KTEEEQFART LERGLALLDE ELAKLSGDTL DGETAFRLYD TYGFPVDLTA DVCRERNIKV DEAGFEAAME EQRRRAREAS GFGADYNAMI RVDSASEFKG YDHLELNGKV TALFVDGKAV DAINAGQEAV VVLDQTPFYA ESGGQVGDKG ELKGANFSFA VEDTQKYGQA IGHIGKLAAG SLKVGDAVQA DVDEARRARI RLNHSATHLM HAALRQVLGT HVSQKGSLVN DKVLRFDFSH NEAMKPEEIR AVEDLVNTQI RRNLPIETNI MDLEAAKAKG AMALFGEKYD ERVRVLSMGD FSTELCGGTH ASRTGDIGLF RIISESGTAA GVRRIEAVTG EGAIATVHAD SDRLSEVAHL LKGDSNNLAD KVRSVLERTR QLEKELQQLK EQAAAQESAN LSSKAIDVNG VKLLVSELSG VEPKMLRTMV DDLKNQLGST IIVLATVVEG KVSLIAGVSK DVTDRVKAGE LIGMVAQQVG GKGGGRPDMA QAGGTDAAAL PAALASVKGW VSAKLQ |
|---|---|
| Mol. Wt |
96.03 kDa (calc) [2] |
| pI | |
| Extinction coefficient |
70820 - 71570 (calc based on 18 Y, 8 W, and 6 C residues) |
| edit table |
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Domains/Motifs/Modification Sites
| Type | Residues | Description | Notes |
|---|---|---|---|
| edit table |
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
Structure
| Structures |
|
|---|---|
| Models |
View models at: |
| edit table |
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.
Notes
Gene Product Resources
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Notes
Accessions in Other Databases
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
See Help:Product_accessions for help entering or editing information in this section of EcoliWiki.
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
See Help:Links_table for how to enter or edit information in this section of EcoliWiki.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 5.0 5.1 Putney SD et al. (1981) Purification and properties of alanine tRNA synthetase from Escherichia coli A tetramer of identical subunits. J Biol Chem 256: 198-204 PubMed EcoliWiki page
- ↑ Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed EcoliWiki page
- ↑ Miller WT et al. (1991) Evidence for a "cysteine-histidine box" metal-binding site in an Escherichia coli aminoacyl-tRNA synthetase. Biochemistry 30: 6970-6 PubMed EcoliWiki page
- ↑ 8.0 8.1 8.2 Putney SD & Schimmel P (1981) An aminoacyl tRNA synthetase binds to a specific DNA sequence and regulates its gene transcription. Nature 291: 632-5 PubMed EcoliWiki page
Categories
Categories: GO:0005524 ! ATP binding | GO:0004813 ! alanine-tRNA ligase activity | GO:0004812 ! aminoacyl-tRNA ligase activity | GO:0003676 ! nucleic acid binding | GO:0000166 ! nucleotide binding | GO:0046872 ! metal ion binding | GO:0005737 ! cytoplasm | GO:0006412 ! translation | GO:0006419 ! alanyl-tRNA aminoacylation | GO:0008270 ! zinc ion binding | GO:0016874 ! ligase activity | GO:0005624 ! membrane fraction | GO:0016566 ! specific transcriptional repressor activity | GO:0045892 ! negative regulation of transcription, DNA-dependent | GO:0006418 ! tRNA aminoacylation for protein translation | Proteins | Complex:alanyl-tRNA synthetase
