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Gene Name

aldA (synonyms: ECK1408, b1415, JW1412, ald)[1]

Product(s)

AldA product[1]: aldehyde dehydrogenase A, NAD-linked product, B1415 product, Ald product

Description

AldA[2][3]

Component of aldehyde dehydrogenase A, NAD-linked[2]

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): aldA[2]

Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name

aldA

Mnemonic
Synonyms

ECK1408, b1415, JW1412, ald[1]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

32.03 minutes 

MG1655: 1486256..1487695
gbrowse_img.php?coord=1485256..1488695&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1489946..1491385
gbrowse_img.php?coord=1488946..1492385&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔaldA (Keio:JW1412)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC9239[5]

ΔaldA776::kan

PMID:16738554[4]

CGSC:99962


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW1412

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCAGTACCCGTTCAACATCC

Primer 2:CCAGACTGTAAATAAACCACCTG

zhj-3076::Tn10

Linked marker

CAG18640 = CGSC7453[5]

est. P1 cotransduction: 8% [7]

trg-2::Tn10

Linked marker

CAG12026 = CGSC7376[5]

est. P1 cotransduction: 77% [7]

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10035

EchoBASE (EcoliWiki Page)

EchoBASE:EB0034

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1415

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1842

EcoGene (EcoliWiki Page)

EcoGene:EG10035

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ALDA

CGSC (EcoliWiki Page)

CGSC:17767

ASAP (EcoliWikiPage)

ASAP:ABE-0004725


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Notes

Links

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NameURLComments
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Categories

Product(s)

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Nomenclature

Standard name

AldA

Synonyms

aldehyde dehydrogenase A, NAD-linked[1], B1415[2][1], Ald[2][1], AldA[2][1]

Product description

AldA[2][3];

Component of ; aldehyde dehydrogenase A, NAD-linked[2][3]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0016052

carbohydrate catabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009438

methylglyoxal metabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0006113

fermentation

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009441

glycolate metabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0008911

lactaldehyde dehydrogenase activity

PMID:4310089[8]

IDA: Inferred from Direct Assay

F

complete

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Interactions

Partner TypePartnerNotesReferences

Protein

Subunits of aldehyde dehydrogenase A, NAD-linked

could be indirect


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Notes

Localization

CompartmentDescriptionEvidenceNotes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence

at EcoCyc 

MSVPVQHPMY IDGQFVTWRG DAWIDVVNPA TEAVISRIPD GQAEDARKAI DAAERAQPEW
EALPAIERAS WLRKISAGIR ERASEISALI VEEGGKIQQL AEVEVAFTAD YIDYMAEWAR
RYEGEIIQSD RPGENILLFK RALGVTTGIL PWNFPFFLIA RKMAPALLTG NTIVIKPSEF
TPNNAIAFAK IVDEIGLPRG VFNLVLGRGE TVGQELAGNP KVAMVSMTGS VSAGEKIMAT
AAKNITKVCL ELGGKAPAIV MDDADLELAV KAIVDSRVIN SGQVCNCAER VYVQKGIYDQ
FVNRLGEAMQ AVQFGNPAER NDIAMGPLIN AAALERVEQK VARAVEEGAR VAFGGKAVEG
KGYYYPPTLL LDVRQEMSIM HEETFGPVLP VVAFDTLEDA ISMANDSDYG LTSSIYTQNL
NVAMKAIKGL KFGETYINRE NFEAMQGFHA GWRKSGIGGA DGKHGLHEYL QTQVVYLQS
Mol. Wt

52.27 kDa (calc) [2]

pI

5.24[2]

Extinction coefficient

59360 - 59735 (calc based on 14 Y, 7 W, and 3 C residues)

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Domains/Motifs/Modification Sites

TypeResiduesDescriptionNotes
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Structure

Structures
  • Representative Escherichia coli structure:
    2HG2|A
    1-479 of 479 residues (E-value: 3.9e-255) (Percent Identity: 100.00)
  • Representative homolog:
    2ESD|A (Streptococcus mutans)
    4-453 of 479 residues (E-value: 1.3e-62) (Percent Identity: 33.48)
  • View all other structures.
Models

View models at:

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Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:LACTALDDEHYDROG-MONOMER

UniProt (EcoliWiki Page)

UniProt:P25553

PFAM (EcoliWiki Page)

PFAM:PF00171

RefSeq (EcoliWiki Page)

RefSeq:NP_415933

ModBase (EcoliWiki Page)

ModBase:P25553


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Notes

Links

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NameURLComments
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Categories

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)

aldA

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Figure courtesy of RegulonDB
Figure courtesy of RegulonDB

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=1486236..1486276&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele NameMutationPhenotypeReference
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Expression Studies

TypeReferenceNotes

microarray

GenExpDB:b1415 (EcoliWiki Page)

Summary of data for aldA from multiple microarray studies

microarray

GEO Profiles:b1415 (EcoliWiki Page)

NCBI GEO profiles for aldA

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Expression Resources

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (1485974..1486312) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ03; Well:G6[9]

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Notes

Accessions Related to aldA Expression

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10035

EchoBASE (EcoliWiki Page)

EchoBASE:EB0034

EcoliGenExpDB (EcoliWiki Page)

GenExpDB:b1415

EcoGene (EcoliWiki Page)

EcoGene:EG10035

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Notes

Links

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NameURLComments
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Categories

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Evolution

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Homologs in Other Organisms

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OrganismHomologs (Statistics)Comments

Shigella flexneri

ALDA

From SHIGELLACYC

E. coli O157

ALDA

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00171

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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  8. ↑ Sridhara S & Wu TT (1969) Purification and properties of lactaldehyde dehydrogenase from Escherichia coli. J Biol Chem 244: 5233-8 PubMed EcoliWiki page
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

Categories

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