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aldB:On One Page

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Gene Name	aldB (synonyms: ECK3577, b3588, JW3561, yiaX)^[1]
Product(s)	AldB product^[1]: aldehyde dehydrogenase B product, B3588 product, YiaX product
Description	ALDDEHYDROGB-MONOMER^[2]^[3] Component of acetaldehyde dehydrogenase^[3]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aldB^[2], OP00186, aldb
Regulation/Activity
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Notes

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Gene

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Nomenclature

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Standard name	aldB
Mnemonic
Synonyms	ECK3577, b3588, JW3561, yiaX^[1]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	80.89 minutes	MG1655: 3754534..3752996	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 3883904..3885442	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

80.89 minutes

MG1655: 3754534..3752996
gbrowse_img.php?coord=3751996..3755534&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 3883904..3885442
gbrowse_img.php?coord=3882904..3886442&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔaldB (Keio:JW3561)	deletion	deletion	PMID:16738554^[4]	Shigen CGSC10626^[5]
ΔaldB772::kan			PMID:16738554^[4]	CGSC:99964
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691^[6] Status: Primer 1: Primer 2:
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: % ^[7] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: 1% ^[7]
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG12292
EchoBASE (EcoliWiki Page)	EchoBASE:EB2200
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3588
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C4408
EcoGene (EcoliWiki Page)	EcoGene:EG12292
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:ALDB
CGSC (EcoliWiki Page)	CGSC:35668
ASAP (EcoliWikiPage)	ASAP:ABE-0011718
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	AldB
Synonyms	aldehyde dehydrogenase B^[1], B3588^[2]^[1], YiaX^[2]^[1], AldB^[2]^[1]
Product description	ALDDEHYDROGB^[2]^[3]; Component of ; acetaldehyde dehydrogenase^[3]
EC number (for enzymes)	1.2.1.22^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0016491	oxidoreductase activity	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR015590 SP_KW:KW-0560	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0008152	metabolic process	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015590	P	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0000287	magnesium ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005515	protein binding	PMID:16606699^[9]	IPI: Inferred from Physical Interaction	UniProtKB:P0A6Y8	F	Seeded from EcoCyc 12.5 ^[8]	complete
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	required fields missing
	GO:0016052	carbohydrate catabolic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0009438	methylglyoxal metabolic process				P	Seeded from Riley et al 2006 ^[1].	required fields missing
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Interactions

Partner Type	Partner	Notes	References
Protein	Subunits of acetaldehyde dehydrogenase	could be indirect
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Notes

Localization

Compartment	Description	Evidence	Notes
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Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MIAVVKLLPT GEDFASHVYP CYTSHRKETI MTNNPPSAQI KPGEYGFPLK LKARYDNFIG GEWVAPADGE YYQNLTPVTG QLLCEVASSG KRDIDLALDA AHKVKDKWAH TSVQDRAAIL FKIADRMEQN LELLATAETW DNGKPIRETS AADVPLAIDH FRYFASCIRA QEGGISEVDS ETVAYHFHEP LGVVGQIIPW NFPLLMASWK MAPALAAGNC VVLKPARLTP LSVLLLMEIV GDLLPPGVVN VVNGAGGVIG EYLATSKRIA KVAFTGSTEV GQQIMQYATQ NIIPVTLELG GKSPNIFFAD VMDEEDAFFD KALEGFALFA FNQGEVCTCP SRALVQESIY ERFMERAIRR VESIRSGNPL DSVTQMGAQV SHGQLETILN YIDIGKKEGA DVLTGGRRKL LEGELKDGYY LEPTILFGQN NMRVFQEEIF GPVLAVTTFK TMEEALELAN DTQYGLGAGV WSRNGNLAYK MGRGIQAGRV WTNCYHAYPA HAAFGGYKQS GIGRETHKMM LEHYQQTKCL LVSYSDKPLG LF
Mol. Wt	56.31 kDa (calc) ^[2]
pI
Extinction coefficient	69790 - 70790 (calc based on 21 Y, 7 W, and 8 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
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Structure

Structures	Representative homolog: 1EYY\|A (Vibrio harveyi) 31-398 of 542 residues (E-value: 1.0e-06) (Percent Identity: 23.91) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative homolog:
1EYY|A (Vibrio harveyi)
31-398 of 542 residues (E-value: 1.0e-06) (Percent Identity: 23.91)
View all other structures.

Models

View models at:

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:ALDDEHYDROGB-MONOMER
UniProt (EcoliWiki Page)	UniProt:P37685
PFAM (EcoliWiki Page)	PFAM:PF00171
RefSeq (EcoliWiki Page)	RefSeq:NP_418045
ModBase (EcoliWiki Page)	ModBase:P37685
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
aldB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=3754514..3754554&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled+flip&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	GenExpDB:b3588 (EcoliWiki Page)	Summary of data for aldB from multiple microarray studies
microarray	GEO Profiles:b3588 (EcoliWiki Page)	NCBI GEO profiles for aldB
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3754148..3754379) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ12; Well:B12^[10]
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Notes

Accessions Related to aldB Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG12292
EchoBASE (EcoliWiki Page)	EchoBASE:EB2200
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b3588
EcoGene (EcoliWiki Page)	EcoGene:EG12292
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Schizosaccharomyces pombe	SPAC9E99c (score: 1.000; bootstrap: 95%)	From Inparanoid:20070104
Shigella flexneri	ALDB	From SHIGELLACYC
E. coli O157	ALDB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF00171
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Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^8.0 ^8.1 ^8.2 ^8.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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Retrieved from "http://ecoliwiki.net/colipedia/index.php/aldB:On_One_Page"

aldB:On One Page

From EcoliWiki

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aldB Expression

Notes

Links

Categories

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

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References

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