alkB:Gene - EcoliWiki
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alkB:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

alkB

Mnemonic
Synonyms

ECK2204, b2212, JW2200, aidD[1]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

49.72 minutes, 49.72 minutes 

MG1655: 2307363..2306713
gbrowse_img.php?coord=2305713..2308363&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2312675..2312025
gbrowse_img.php?coord=2311025..2313675&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔalkB (Keio:JW2200)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9779[3]

ΔalkB765::kan

PMID:16738554[2]

CGSC:99968


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW2200

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCTTGGATCTGTTTGCCGATGC

Primer 2:CCTTCTTTTTTACCTGCCTGACG

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[3]

est. P1 cotransduction: 83% [5]

Synonyms:zeg-722, zei-722::Tn10

atoS298::Tn10

Linked marker

CAG12177 gyrA+ = CGSC7525[3]

est. P1 cotransduction: 63% [5]

Synonyms:zeh-298::Tn10, zej-298::Tn10


The original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10037

EchoBASE (EcoliWiki Page)

EchoBASE:EB0036

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2212

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2753

EcoGene (EcoliWiki Page)

EcoGene:EG10037

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ALKB

CGSC (EcoliWiki Page)

CGSC:18544

ASAP (EcoliWikiPage)

ASAP:ABE-0007308


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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