amiC:Gene - EcoliWiki
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amiC:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

amiC

Mnemonic
Synonyms

ECK2813, b2817, JW5449, ygdN[1]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

63.49 minutes 

MG1655: 2947032..2945779
gbrowse_img.php?coord=2944779..2948032&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2947666..2946413
gbrowse_img.php?coord=2945413..2948666&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔamiC (Keio:JW5449)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC11395[3]

ΔamiC742::kan

PMID:16738554[2]

CGSC:99994

∆amiC::Kan

Colony Morphology

20-30% of the population exists as chains of 3 to 6 unseparated cells.

PMID:11454209[4]


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW5449

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTCAGGATCCAACACTGCAAT

Primer 2:CCTCCCCTTCTCGCCAGCGTCGC

fucP3072::Tn10

Linked marker

CAG12079 = CGSC7427[3]

est. P1 cotransduction: 62% [6]

Synonyms:fuc-3072::Tn10

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[3]

est. P1 cotransduction: 85% [6]

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7458

EchoBASE (EcoliWiki Page)

EchoBASE:EB2895

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2817

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3411

EcoGene (EcoliWiki Page)

EcoGene:EG13086

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z4134

ASAP (EcoliWikiPage)

ASAP:ABE-0009238


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Heidrich C et al. (2001) Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli. Mol Microbiol 41: 167-78 PubMed EcoliWiki page
  5. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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