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arnA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

arnA

Mnemonic

ara4N (4-amino-4-deoxy-L-arabinose)

Synonyms

yfbG, ECK2248, b2255, JW2249, pmrI, SAF[1][2], pbgP3, SAF

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

51 minutes 

MG1655: 2366061..2368043


REL606

NC_012967: 2314552..2316534


BW2952

NC_012759: 2251866..2253848


W3110

 

W3110: 2372709..2374691


DH10B

DH10B: 2457049..2459031


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2366061

Edman degradation

PMID:11248028[3]


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

arnAR619M

R619M

400-fold lower activity

seeded from UniProt:P77398

arnAR619E,Y

R619E,Y

No activity

seeded from UniProt:P77398

arnAE434Q

E434Q

No activity

seeded from UniProt:P77398

arnAS433T

S433T

No activity

seeded from UniProt:P77398

arnAE434A

E434A

100-fold lower specific activity

seeded from UniProt:P77398

arnAD140A,N

D140A,N

Less than 1% residual activity

seeded from UniProt:P77398

arnAS433A

S433A

40-fold lower specific activity

seeded from UniProt:P77398

arnAH104K

H104K

Less than 1% residual activity

seeded from UniProt:P77398

arnAN102A

N102A

No activity

seeded from UniProt:P77398

arnAH104A

H104A

25-fold lower activity

seeded from UniProt:P77398

ΔarnA736::kan

PMID:16738554[4]

CGSC:106130


Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[5]

Status:

Primer 1:

Primer 2:

4B4

Kohara Phage

Genobase

PMID:3038334[6]

5H12

Kohara Phage

Genobase

PMID:3038334[6]

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7168

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14091

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003744

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947683

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3844

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007460

Escherichia coli str. K-12 substr. MG1655

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Karzai, AW & Sauer, RT (2001) Protein factors associated with the SsrA.SmpB tagging and ribosome rescue complex. Proc. Natl. Acad. Sci. U.S.A. 98 3040-4 PubMed EcoliWiki page
  4. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed EcoliWiki page
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed EcoliWiki page
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed EcoliWiki page

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