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ascG:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ascG

Mnemonic

Arbutin, salicin, cellobiose

Synonyms

ECK2709, b2714, JW5434, sac[1], sac

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

61.13 minutes 

MG1655: 2837289..2836276


REL606

NC_012967: 2734127..2733114


BW2952

NC_012759: 2722088..2723101


W3110

 

W3110: 2837923..2836910


DH10B

DH10B: 2929831..2928818


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔascG (Keio:JW5434)

deletion

deletion

PMID:16738554[2]

Shigen

ascG::Tn5KAN-I-SceI (FB20893)

Insertion at nt 251 in Plus orientation

PMID:15262929[3]

E. coli Genome Project:FB20893

does not contain pKD46

ascG::Tn5KAN-I-SceI (FB20894)

Insertion at nt 251 in Plus orientation

PMID:15262929[3]

E. coli Genome Project:FB20894

contains pKD46

Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5434

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCATGACGACGATGCTGGAAGT

Primer 2:CCTCGCGAAGGAGCAATGAGTGA

9G10

Kohara Phage

Genobase

PMID:3038334[5]

1B2

Kohara Phage

Genobase

PMID:3038334[5]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[6]

est. P1 cotransduction: 69% [7]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

cysI95::Tn10

Linked marker

CAG12173 = CGSC7425[6]

est. P1 cotransduction: 10% [7]
Synonyms:cysC95::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10087

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10087

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000083

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947305

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0085

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008922

Escherichia coli str. K-12 substr. MG1655

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed EcoliWiki page
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed EcoliWiki page
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed EcoliWiki page
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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