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asnA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

asnA

Mnemonic

Asparagine

Synonyms

ECK3738, b3744, JW3722[1], JW3722

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

84.6 minutes 

MG1655: 3925178..3926170


REL606

NC_012967: 3887516..3888508


BW2952

NC_012759: 3813511..3814503


W3110

 

W3110: 3709526..3708534


DH10B

DH10B: 4022762..4023754


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔasnA (Keio:JW3722)

deletion

deletion

Auxotrophies

PMID:16738554[2]

Shigen CGSC10719[3]

asnA::Tn5KAN-I-SceI (FB21433)

Insertion at nt 517 in Plus orientation

Auxotrophies

PMID:15262929[4]

E. coli Genome Project:FB21433

contains pKD46

asnA31

Auxotrophies

leaky for the Asn- phenotype

PMID:6102983[5]

CGSC:5119

ΔasnA773::kan

deletion

deletion

Auxotrophies

PMID:16738554[2]

CGSC:100104


Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3722

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAACCGCTTACATTGCCAA

Primer 2:CCaAGCAGAGAAGGGACGCTCTC

4F4

Kohara Phage

Genobase

PMID:3038334[7]

2A1

Kohara Phage

Genobase

PMID:3038334[7]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[3]

est. P1 cotransduction: 9% [8]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[3]

est. P1 cotransduction: 86% [8]
Synonyms:zie-296::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10091

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10091

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000087

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948258

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0089

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012242

Escherichia coli str. K-12 substr. MG1655

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed EcoliWiki page
  5. Felton, J et al. (1980) Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli. J. Bacteriol. 142 221-8 PubMed EcoliWiki page
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed EcoliWiki page
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed EcoliWiki page
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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