atoS:Gene - EcoliWiki
EcoliHub logo

E. coli is indeed the coolest thing on the planet - Carl Zimmer

atoS:Gene

From EcoliWiki

Jump to: navigation, search


Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


Standard name

atoS

Mnemonic
Synonyms

ECK2212, b2219, JW2213[1]

edit table

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

[back to top]


StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

49.96 minutes 

MG1655: 2318065..2319891
gbrowse_img.php?coord=2317065..2320891&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2324713..2326539
gbrowse_img.php?coord=2323713..2327539&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

edit table

See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

[back to top]


Feature typeLocationDescription
edit table

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

[back to top]


AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔatoS (Keio:JW2213)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9784[3]

atoS298::Tn10

Insertion at 2,318,296 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12177 gyrA = CGSC7525[3]

Synonyms: zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

atoS298::Tn10

PMID:2540407[5] PMID:9829956[6]

CGSC:8751

atoS3142::Tn10kan

PMID:2540407[5] PMID:9829956[6]

CGSC:40008

ΔatoS773::kan

PMID:16738554[2]

CGSC:100132


edit table

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

[back to top]


ResourceResource TypeSourceNotes/Reference

JW2213

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCCATTATATGAAGTGGATTTA

Primer 2:CCTACAGTCTGATTTCCCTGCGG

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[3]

est. P1 cotransduction: 55% [4]

Synonyms:zeg-722, zei-722::Tn10

zfa-723::Tn10

Linked marker

CAG12178 gyrA+ = CGSC7526[3]

est. P1 cotransduction: 39% [4]

Synonyms:zei-723::Tn10, zfa-723::Tn10


The original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

edit table

See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

[back to top]


DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11667

EchoBASE (EcoliWiki Page)

EchoBASE:EB1618

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2219

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2762

EcoGene (EcoliWiki Page)

EcoGene:EG11667

CGSC (EcoliWiki Page)

CGSC:37061

ASAP (EcoliWikiPage)

ASAP:ABE-0007341


edit table

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

[back to top]


NameURLComments
edit table

See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. 5.0 5.1 Singer M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev 53: 1-24 PubMed EcoliWiki page
  6. 6.0 6.1 Nichols BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J Bacteriol 180: 6408-11 PubMed EcoliWiki page
  7. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page

Categories

Personal tools