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btuD:On One Page

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Standard Name

btuD

Gene Synonym(s)

ECK1707, b1709, JW1699[1]

Product Desc.

BtuD[2][3];

Component of vitamin B12 ABC transporter[2][3]; vitamin B12 transport system[3]

B12 uptake, ABC transporter permease[4]

Product Synonyms(s)

vitamin B12 transporter subunit : ATP-binding component of ABC superfamily[1], B1709[2][1], BtuD[2][1]

Function from GO

Cellular Component

Biological Process

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): btuCED[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(btuD)    Textpresso(btuD)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

btuD

Mnemonic

B12 uptake

Synonyms

ECK1707, b1709, JW1699[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

38.6 minutes 

MG1655: 1791582..1790833
gbrowse_img.php?coord=1790833..1791582&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1770825..1770076
gbrowse_img.php?coord=1770076..1770825&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1795272..1794523
gbrowse_img.php?coord=1794523..1795272&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1882153..1881404
gbrowse_img.php?coord=1881404..1882153&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 1682892..1683641
gbrowse_img.php?coord=1682892..1683641&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔbtuD (Keio:JW1699)

deletion

deletion

PMID:16738554

Shigen
CGSC9438[5]

btuD

PMID:12519969

ASAP

At position 105 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20435

ΔbtuD783::kan

PMID:16738554

CGSC:100216


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW1699

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCTATTGTGATGCAGTTACA

Primer 2:CCGATGGTCGAAATCAGCATTCT

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: 62% [6]
Synonyms:zdh-925::Tn10

zdj-276::Tn10

Linked marker

CAG18464 = CGSC7387[5]

est. P1 cotransduction: 17% [6]
Synonyms:zdi-276::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10128

EchoBASE (EcoliWiki Page)

EchoBASE:EB0126

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1709

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2105

EcoGene (EcoliWiki Page)

EcoGene:EG10128

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:BTUD

CGSC (EcoliWiki Page)

CGSC:18499

ASAP (EcoliWikiPage)

ASAP:ABE-0005707


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

BtuD

Synonyms

vitamin B12 transporter subunit : ATP-binding component of ABC superfamily[1], B1709[2][1], BtuD[2][1]

Product description

BtuD[2][3];

Component of vitamin B12 ABC transporter[2][3]; vitamin B12 transport system[3]

B12 uptake, ABC transporter permease[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0017111

nucleoside-triphosphatase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc [7]

complete

GO:0016887

ATPase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003439

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc [7]

complete

GO:0015889

cobalamin transport

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01005

P

Seeded from EcoCyc [7]

complete

GO:0016020

membrane

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003593
SP_KW:KW-0547

F

Seeded from EcoCyc [7]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003439
SP_KW:KW-0067

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0006810

transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc [7]

complete

GO:0015420

cobalamin-transporting ATPase activity

GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

EC:3.6.3.33
HAMAP:MF_01005

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005886

plasma membrane

GO_REF:0000020
GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

HAMAP:MF_01005
SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [7]

complete

GO:0009236

cobalamin biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0000300

peripheral to membrane of membrane fraction

PMID:3528128

IDA: Inferred from Direct Assay

C

complete

GO:0015889

cobalamin transport

GO_REF:0000020

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of vitamin B12 ABC transporter

could be indirect

Protein

yfjI

PMID:16606699

Experiment(s):EBI-1140564

Protein

groL

PMID:16606699

Experiment(s):EBI-1140564

Protein

Subunits of vitamin B12 transport system

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

cytoplasm

From EcoCyc[3]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MSIVMQLQDV AESTRLGPLS GEVRAGEILH LVGPNGAGKS TLLARMAGMT SGKGSIQFAG
QPLEAWSATK LALHRAYLSQ QQTPPFATPV WHYLTLHQHD KTRTELLNDV AGALALDDKL
GRSTNQLSGG EWQRVRLAAV VLQITPQANP AGQLLLLDEP MNSLDVAQQS ALDKILSALC
QQGLAIVMSS HDLNHTLRHA HRAWLLKGGK MLASGRREEV LTPPNLAQAY GMNFRRLDIE
GHRMLISTI
Length

249

Mol. Wt

27.081 kDa

pI

9.5 (calculated)

Extinction coefficient

26,470 - 26,595 (calc based on 3 Y, 4 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:BTUD-MONOMER

UniProt (EcoliWiki Page)

UniProt:P06611

PFAM (EcoliWiki Page)

PFAM:PF00005

RefSeq (EcoliWiki Page)

RefSeq:NP_416224


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

btuCED

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=1791562..1791602&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b1709 (EcoliWiki Page)

NCBI GEO profiles for btuD

microarray

GenExpDB:b1709 (EcoliWiki Page)

Summary of data for btuD from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to btuD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10128

EchoBASE (EcoliWiki Page)

EchoBASE:EB0126

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1709

EcoGene (EcoliWiki Page)

EcoGene:EG10128


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Shigella flexneri

BTUD

From SHIGELLACYC

E. coli O157

BTUD

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00005


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ 8.0 8.1 8.2 8.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

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