Try the new EcoliHub and let us know what you'd like to see.
carA:Gene Product(s)
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
CarA |
|---|---|
| Synonyms |
carbamoyl phosphate synthetase small subunit, glutamine amidotransferase[1], B0032[2][1], Arg[2][1], Cap[2][1], PyrA[2][1], α chain[2][1], small chain[2][1], CarA[2][1] |
| Product description |
Component of carbamoyl phosphate synthetase[2][3] Carbamoyl phosphate synthase, small subunit; glutamine-hydrolyzing, tetramer of heterodimers[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004088 |
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0006221 |
pyrimidine nucleotide biosynthetic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0004086 |
carbamoyl-phosphate synthase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0005524 |
ATP binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0006526 |
arginine biosynthetic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0006541 |
glutamine metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [6] |
complete | |||
| GO:0006807 |
nitrogen compound metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [6] |
complete | |||
| GO:0008652 |
amino acid biosynthetic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [6] |
complete | |||
| GO:0009058 |
biosynthetic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [6] |
complete | |||
| GO:0016874 |
ligase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0005737 |
cytoplasm |
C |
Seeded from Riley et al 2006 [1]. |
required fields missing | ||||
| GO:0005829 |
cytosol |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc [6] |
complete | |||
| GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0005515 |
protein binding |
IMP: Inferred from Mutant Phenotype |
F |
CarB |
complete | |||
|
Contributes to | GO:0004088 |
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity |
IMP: Inferred from Mutant Phenotype |
F |
complete | |||
| GO:0006541 |
glutamine metabolic process |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0006526 |
arginine biosynthetic process |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0006207 |
'de novo' pyrimidine base biosynthetic process |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of carbamoyl phosphate synthetase |
could be indirect | ||
|
Protein |
Experiment(s):EBI-880588, EBI-886422 | |||
|
Protein |
Experiment(s):EBI-888940 | |||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR QIVTLTYPHI GNVGTNDADE ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS YLKRHNIVAI ADIDTRKLTR LLREKGAQNG CIIAGDNPDA ALALEKARAF PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG LPEAKKEDEL PFHVVAYDFG AKRNILRMLV DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN GPGDPAPCDY AITAIQKFLE TDIPVFGICL GHQLLALASG AKTVKMKFGH HGGNHPVKDV EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF QGHPEASPGP HDAAPLFDHF IELIEQYRKT AK |
|---|---|
| Length |
382 |
| Mol. Wt |
41.431 kDa |
| pI |
6.3 (calculated) |
| Extinction coefficient |
22,920 - 23,420 (calc based on 8 Y, 2 W, and 4 C residues) |
| edit table |
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
|
|
Structure
|
Structure figures
| ||||||||||||||||||||||||||||
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
- ↑ 7.0 7.1 7.2 7.3 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
- ↑ Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
- ↑ Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed EcoliWiki page
- ↑ 10.0 10.1 Guillou F et al. (1989) Escherichia coli carbamoyl-phosphate synthetase: domains of glutaminase and synthetase subunit interaction. Proc Natl Acad Sci U S A 86: 8304-8 PubMed EcoliWiki page
- ↑ 11.0 11.1 Mullins LS et al. (1991) Alterations in the energetics of the carbamoyl phosphate synthetase reaction by site-directed modification of the essential sulfhydryl group. J Biol Chem 266: 8236-40 PubMed EcoliWiki page
- ↑ 12.0 12.1 Piérard A & Wiame JM (1964) Regulation and mutation affecting a glutamine dependent formation of carbamyl phosphate in Escherichia coli. Biochem Biophys Res Commun 15: 76-81 PubMed EcoliWiki page
