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carA:On One Page

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Standard Name

carA

Gene Synonym(s)

ECK0033, b0032, JW0030, arg+ura, cap, pyrA, arg[1]

Product Desc.

CarA[2][3];

Component of carbamoyl phosphate synthetase[2][3]

Carbamoyl phosphate synthase, small subunit; glutamine-hydrolyzing, tetramer of heterodimers[4]

Product Synonyms(s)

carbamoyl phosphate synthetase small subunit, glutamine amidotransferase[1], B0032[2][1], Arg[2][1], Cap[2][1], PyrA[2][1], α chain[2][1], small chain[2][1], CarA[2][1]

Function from GO

Molecular Function

Cellular Component

Biological Process

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): carAB[2], OP00150, pyrA

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(carA)    Textpresso(carA)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

carA

Mnemonic

Carbamoyl P

Synonyms

ECK0033, b0032, JW0030, arg+ura, cap, pyrA, arg[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

0.64 minutes 

MG1655: 29651..30799
gbrowse_img.php?coord=29651..30799&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 33722..34870
gbrowse_img.php?coord=33722..34870&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 29651..30799
gbrowse_img.php?coord=29651..30799&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 29651..30799
gbrowse_img.php?coord=29651..30799&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 29651..30799
gbrowse_img.php?coord=29651..30799&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔcarA (Keio:JW0030)

deletion

deletion

PMID:16738554

Shigen

arg-47

CGSC:4690

arg-23

CGSC:5987

carA50

CGSC:6597

carA178

CGSC:6600


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0030

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATTAAGTCAGCGCTATTGGT

Primer 2:CCCTTAGCGGTTTTACGGTACTG

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[5]

est. P1 cotransduction: 32% [6]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 91% [6]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10134

EchoBASE (EcoliWiki Page)

EchoBASE:EB0132

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0032

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0040

EcoGene (EcoliWiki Page)

EcoGene:EG10134

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:CARA

CGSC (EcoliWiki Page)

CGSC:936

ASAP (EcoliWikiPage)

ASAP:ABE-0000118


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

CarA

Synonyms

carbamoyl phosphate synthetase small subunit, glutamine amidotransferase[1], B0032[2][1], Arg[2][1], Cap[2][1], PyrA[2][1], α chain[2][1], small chain[2][1], CarA[2][1]

Product description

CarA[2][3];

Component of carbamoyl phosphate synthetase[2][3]

Carbamoyl phosphate synthase, small subunit; glutamine-hydrolyzing, tetramer of heterodimers[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0004088

carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity

GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

EC:6.3.5.5
HAMAP:MF_01209

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0003824

catalytic activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000991
InterPro:IPR006220
InterPro:IPR011702

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0006221

pyrimidine nucleotide biosynthetic process

GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

SP_KW:KW-0665
HAMAP:MF_01209

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0004086

carbamoyl-phosphate synthase activity

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002474
InterPro:IPR006274

F

Seeded from EcoCyc [8]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002474
HAMAP:MF_01209

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0006526

arginine biosynthetic process

GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

SP_KW:KW-0055
HAMAP:MF_01209

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0006541

glutamine metabolic process

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001317
InterPro:IPR011702
SP_KW:KW-0315

P

Seeded from EcoCyc [8]

complete

GO:0006807

nitrogen compound metabolic process

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002474
InterPro:IPR006274

P

Seeded from EcoCyc [8]

complete

GO:0008652

amino acid biosynthetic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0028

P

Seeded from EcoCyc [8]

complete

GO:0009058

biosynthetic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006220

P

Seeded from EcoCyc [8]

complete

GO:0016874

ligase activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001317
SP_KW:KW-0436

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0005515

protein binding

PMID:15690043

IPI: Inferred from Physical Interaction

UniProtKB:P00968

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0005515

protein binding

PMID:15690043

IPI: Inferred from Physical Interaction

UniProtKB:P22188

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0005515

protein binding

PMID:16606699

IPI: Inferred from Physical Interaction

UniProtKB:P46142

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [8]

complete

GO:0005737

cytoplasm

PMID:2682645

IDA: Inferred from Direct Assay

C

complete

GO:0005515

protein binding

PMID:2682645

IMP: Inferred from Mutant Phenotype

F

CarB

complete

Contributes to

GO:0004088

carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity

PMID:1827118

IMP: Inferred from Mutant Phenotype

F

complete

GO:0006541

glutamine metabolic process

PMID:1827118

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006526

arginine biosynthetic process

PMID:5319709

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006207

'de novo' pyrimidine base biosynthetic process

PMID:5319709

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of carbamoyl phosphate synthetase

could be indirect

Protein

carB

PMID:15690043

Experiment(s):EBI-880588, EBI-886422

Protein

murE

PMID:15690043

Experiment(s):EBI-888940

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR QIVTLTYPHI
GNVGTNDADE ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS YLKRHNIVAI ADIDTRKLTR
LLREKGAQNG CIIAGDNPDA ALALEKARAF PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG
LPEAKKEDEL PFHVVAYDFG AKRNILRMLV DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN
GPGDPAPCDY AITAIQKFLE TDIPVFGICL GHQLLALASG AKTVKMKFGH HGGNHPVKDV
EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF QGHPEASPGP
HDAAPLFDHF IELIEQYRKT AK
Length

382

Mol. Wt

41.431 kDa

pI

6.3 (calculated)

Extinction coefficient

22,920 - 23,420 (calc based on 8 Y, 2 W, and 4 C residues)

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Domains/Motifs/Modification Sites

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TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:CARBPSYN-SMALL

UniProt (EcoliWiki Page)

UniProt:P0A6F1

PFAM (EcoliWiki Page)

PFAM:PF00988

RefSeq (EcoliWiki Page)

RefSeq:NP_414573


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

carAB

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=29631..29671&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0032 (EcoliWiki Page)

NCBI GEO profiles for carA

microarray

GenExpDB:b0032 (EcoliWiki Page)

Summary of data for carA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to carA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10134

EchoBASE (EcoliWiki Page)

EchoBASE:EB0132

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0032

EcoGene (EcoliWiki Page)

EcoGene:EG10134


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Arabidopsis thaliana

  • AT3G27740 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6YV24 (score: 1.000; bootstrap: 100%)
  • Q8S3R8 (score: 0.905)
  • Q6YV23 (score: 0.770)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YOR303W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC56F29c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

CARA

From SHIGELLACYC

E. coli O157

CARA

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00988


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Notes

Links

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References

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  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70

Categories

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