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casD:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

casD

Mnemonic

CRISPR associated CRISP-associated

Synonyms

ECK2752, b2757, JW5844, ygcI[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

62.04 minutes 

MG1655: 2879070..2878396
gbrowse_img.php?coord=2878396..2879070&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2879704..2879030
gbrowse_img.php?coord=2879030..2879704&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2971612..2970938
gbrowse_img.php?coord=2970938..2971612&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2764208..2764882
gbrowse_img.php?coord=2764208..2764882&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔygcI (Keio:JW5844)

deletion

deletion

PMID:16738554[2]

Shigen


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW5844

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCAGATCTTATTTGATCTTGCG

Primer 2:CCtTGAGATACATCCATACCTCC

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[4]

est. P1 cotransduction: 8% [5]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

cysI95::Tn10

Linked marker

CAG12173 = CGSC7425[4]

est. P1 cotransduction: 78% [5]
Synonyms:cysC95::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7427

EchoBASE (EcoliWiki Page)

EchoBASE:EB2917

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2757

EcoGene (EcoliWiki Page)

EcoGene:EG13116

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z4066

ASAP (EcoliWikiPage)

ASAP:ABE-0009044


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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