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clpX:Gene
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
clpX |
|---|---|
| Mnemonic |
Caseinolytic protease |
| Synonyms |
ECK0432, b0438, JW0428, LopC[1] |
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Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
9.84 minutes |
MG1655: 456650..457924 | ||
|
NC_012967: 428131..429405 | ||||
|
W3110 |
|
W3110: 456650..457924 | ||
|
DH10B: 395981..397255 | ||||
|
NC_012759: 359409..360683 | ||||
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature type | Location | Description |
|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔclpX (Keio:JW0428) |
deletion |
deletion | |||||
|
At position 641 in Plus orientation, does not contain plasmid pKD46. | |||||||
|
E185Q |
E185Q |
Other |
Loss of ATP hydrolysis in vitro |
Mutant protein retains normal assembly, ATP binding , and the ability to bind polypeptide substrates | |||
|
clpX103(del-ins)::kan | |||||||
|
ΔclpX724::kan |
| ||||||
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0428 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACAGATAAACGCAAAGATGG Primer 2:CCTTCACCAGATGCCTGTTGCGC | |
|
Linked marker |
est. P1 cotransduction: 39% [8] | ||
|
Linked marker |
est. P1 cotransduction: 92% [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Glasner JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res 31: 147-51 PubMed EcoliWiki page
- ↑ Hersch GL et al. (2005) Asymmetric interactions of ATP with the AAA+ ClpX6 unfoldase: allosteric control of a protein machine. Cell 121: 1017-27 PubMed EcoliWiki page
- ↑ Gottesman S et al. (1993) ClpX, an alternative subunit for the ATP-dependent Clp protease of Escherichia coli. Sequence and in vivo activities. J Biol Chem 268: 22618-26 PubMed EcoliWiki page
- ↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
