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cysI:On One Page

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Standard Name

cysI

Gene Synonym(s)

ECK2758, b2763, JW2733[1]

Product Desc.

sulfite reductase hemoprotein subunit[2][3];

Component of SULFITE-REDUCT-CPLX[2]

Sulfite reductase [NADPH] hemoprotein beta-component; has 4Fe-4S iron-sulfur center[4]

Product Synonyms(s)

sulfite reductase, beta subunit, NAD(P)-binding, heme-binding[1], B2763[2][1], CysI[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): cysJIH[2], OP00106, cysJHI

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(cysI)    Textpresso(cysI)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cysI

Mnemonic

Cysteine

Synonyms

ECK2758, b2763, JW2733[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

62.21 minutes 

MG1655: 2888121..2886409
gbrowse_img.php?coord=2886409..2888121&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2778599..2776887
gbrowse_img.php?coord=2776887..2778599&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2888755..2887043
gbrowse_img.php?coord=2887043..2888755&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

BW2952

NC_012759: 2772221..2773933
gbrowse_img.php?coord=2772221..2773933&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔcysI (Keio:JW2733)

deletion

deletion

PMID:16738554

Shigen
CGSC10149[5]

cysI::Tn5KAN

PMID:12519969

ASAP

At position 625 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20925

cysI95::Tn10

Insertion at 2,886,784 bp in MG1655 (NC_000913)

adapted from Nichols et al.[6]

CAG12173 = CGSC7425[5]

Synonyms: cysC95::Tn10

cysI53(Am)

CGSC:8520

amber (UAG) mutation

cysI95::Tn10

PMID:9829956

CGSC:8856

cysI3152::Tn10kan

PMID:2540407 PMID:9829956

CGSC:40100

ΔcysI765::kan

PMID:16738554

CGSC:100494


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW2733

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGCGAAAAACATCCAGGGCC

Primer 2:CCATCCCACAAATCACGCGCCGG

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[5]

est. P1 cotransduction: 4% [6]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

fucP3072::Tn10

Linked marker

CAG12079 = CGSC7427[5]

est. P1 cotransduction: 13% [6]
Synonyms:fuc-3072::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10190

EchoBASE (EcoliWiki Page)

EchoBASE:EB0187

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2763

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3322

EcoGene (EcoliWiki Page)

EcoGene:EG10190

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:CYSI

CGSC (EcoliWiki Page)

CGSC:891

ASAP (EcoliWikiPage)

ASAP:ABE-0009061


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

CysI

Synonyms

sulfite reductase, beta subunit, NAD(P)-binding, heme-binding[1], B2763[2][1], CysI[2][1]

Product description

sulfite reductase hemoprotein subunit[2][3];

Component of SULFITE-REDUCT-CPLX[2]

Sulfite reductase [NADPH] hemoprotein beta-component; has 4Fe-4S iron-sulfur center[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0004783

sulfite reductase (NADPH) activity

GO_REF:0000002
GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR011786
EC:1.8.1.2
HAMAP:MF_01540

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0000103

sulfate assimilation

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01540

P

Seeded from EcoCyc [8]

complete

GO:0005506

iron ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc [8]

complete

GO:0008652

amino acid biosynthetic process

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR011786
SP_KW:KW-0028

P

Seeded from EcoCyc [8]

complete

GO:0009337

sulfite reductase complex (NADPH)

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011786

C

Seeded from EcoCyc [8]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR005117
InterPro:IPR006066
InterPro:IPR006067
SP_KW:KW-0560

F

Seeded from EcoCyc [8]

complete

GO:0019344

cysteine biosynthetic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0198

P

Seeded from EcoCyc [8]

complete

GO:0020037

heme binding

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006066
InterPro:IPR006067

F

Seeded from EcoCyc [8]

complete

GO:0046872

metal ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc [8]

complete

GO:0050661

NADP or NADPH binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011786

F

Seeded from EcoCyc [8]

complete

GO:0051536

iron-sulfur cluster binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR006066
InterPro:IPR006067
SP_KW:KW-0411

F

Seeded from EcoCyc [8]

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR011786
SP_KW:KW-0004

F

Seeded from EcoCyc [8]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0009055

electron carrier activity

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006066
InterPro:IPR006067

F

Seeded from EcoCyc [8]

complete

GO:0055114

oxidation reduction

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc [8]

complete

GO:0006790

sulfur metabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of SULFITE-REDUCT-CPLX

could be indirect

Protein

sdhA

PMID:16606699

Experiment(s):EBI-1143877

Protein

proS

PMID:16606699

Experiment(s):EBI-1143877

Protein

recJ

PMID:16606699

Experiment(s):EBI-1143877

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MSEKHPGPLV VEGKLTDAER MKHESNYLRG TIAEDLNDGL TGGFKGDNFL LIRFHGMYQQ
DDRDIRAERA EQKLEPRHAM LLRCRLPGGV ITTKQWQAID KFAGENTIYG SIRLTNRQTF
QFHGILKKNV KPVHQMLHSV GLDALATAND MNRNVLCTSN PYESQLHAEA YEWAKKISEH
LLPRTRAYAE IWLDQEKVAT TDEEPILGQT YLPRKFKTTV VIPPQNDIDL HANDMNFVAI
AENGKLVGFN LLVGGGLSIE HGNKKTYART ASEFGYLPLE HTLAVAEAVV TTQRDWGNRT
DRKNAKTKYT LERVGVETFK AEVERRAGIK FEPIRPYEFT GRGDRIGWVK GIDDNWHLTL
FIENGRILDY PARPLKTGLL EIAKIHKGDF RITANQNLII AGVPESEKAK IEKIAKESGL
MNAVTPQREN SMACVSFPTC PLAMAEAERF LPSFIDNIDN LMAKHGVSDE HIVMRVTGCP
NGCGRAMLAE VGLVGKAPGR YNLHLGGNRI GTRIPRMYKE NITEPEILAS LDELIGRWAK
EREAGEGFGD FTVRAGIIRP VLDPARDLWD
Length

570

Mol. Wt

63.999 kDa

pI

7.7 (calculated)

Extinction coefficient

64,860 - 65,610 (calc based on 14 Y, 8 W, and 6 C residues)

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Domains/Motifs/Modification Sites

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TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed

UniProt:P17846


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Structure
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Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:BETACOMP-MONOMER

UniProt (EcoliWiki Page)

UniProt:P17846

PFAM (EcoliWiki Page)

PFAM:PF03460

RefSeq (EcoliWiki Page)

RefSeq:NP_417243


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

cysJIH

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2888101..2888141&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b2763 (EcoliWiki Page)

NCBI GEO profiles for cysI

microarray

GenExpDB:b2763 (EcoliWiki Page)

Summary of data for cysI from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to cysI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10190

EchoBASE (EcoliWiki Page)

EchoBASE:EB0187

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2763

EcoGene (EcoliWiki Page)

EcoGene:EG10190


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Arabidopsis thaliana

  • AT5G04590 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Oryza gramene

  • Q60EA3 (score: 1.000; bootstrap: 99%)
  • O23813 (score: 0.890)
  • Q60EI0 (score: 0.869)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YJR137C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC10F61c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000009488 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

CYSI

From SHIGELLACYC

E. coli O157

CYSI

From ECOO157CYC

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Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF03460


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Notes

Links

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References

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  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70

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