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dam:Gene Product(s)
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
Dam |
|---|---|
| Synonyms | |
| Product description |
DNA-(adenine-N6)-methyltransferase[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0009007 |
site-specific DNA-methyltransferase (adenine-specific) activity |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002294 |
F |
Seeded from EcoCyc 12.5 [5] |
complete | ||
| GO:0016740 |
transferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0032259 |
methylation |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [6] |
complete | |||
| GO:0008168 |
methyltransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0006260 |
DNA replication |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [6] |
complete | |||
| GO:0006306 |
DNA methylation |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [6] |
complete | |||
| GO:0003677 |
DNA binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0003676 |
nucleic acid binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [6] |
complete | |||
| GO:0009007 |
site-specific DNA-methyltransferase (adenine-specific) activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0006298 |
mismatch repair |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0006298 |
mismatch repair |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc 12.5 [5] |
complete | |||
| GO:0005737 |
cytoplasm |
C |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
|
NOT | GO:0009307 |
DNA restriction-modification system |
P |
Seeded from EcoCyc 11.1[3]. Dam methylation is not a component of the E. coli restriction-modification systems, although it does block cleavage in vitro by some Type II restriction endonucleases. Dam functions as a component of methyl-directed mismatch repair. |
required field missing | |||
| GO:0009007 |
site-specific DNA-methyltransferase (adenine-specific) activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0006298 |
mismatch repair |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0006298 |
mismatch repair |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0006261 |
DNA-dependent DNA replication |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Experiment(s):EBI-881035 | |||
|
Protein |
Experiment(s):EBI-881035, EBI-887858 | |||
|
Protein |
Experiment(s):EBI-881035 | |||
|
Protein |
Experiment(s):EBI-887858 | |||
|
Protein |
Experiment(s):EBI-887858 | |||
|
Protein |
Experiment(s):EBI-887858 | |||
|
Protein |
Experiment(s):EBI-887858 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
|
Protein |
Experiment(s):EBI-1145744 | |||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS ISSNGGTRKK VDELLALYKP GVVSPAKK |
|---|---|
| Length |
278 |
| Mol. Wt |
32.1 kDa |
| pI |
8.8 (calculated) |
| Extinction coefficient |
40,800 - 41,425 (calc based on 20 Y, 2 W, and 5 C residues) |
| edit table |
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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Structure
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Structure figures
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| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
- ↑ 7.0 7.1 Herman GE & Modrich P (1982) Escherichia coli dam methylase. Physical and catalytic properties of the homogeneous enzyme. J Biol Chem 257: 2605-12 PubMed EcoliWiki page
- ↑ 8.0 8.1 Pukkila PJ et al. (1983) Effects of high levels of DNA adenine methylation on methyl-directed mismatch repair in Escherichia coli. Genetics 104: 571-82 PubMed EcoliWiki page
- ↑ 9.0 9.1 Lu AL et al. (1983) Methyl-directed repair of DNA base-pair mismatches in vitro. Proc Natl Acad Sci U S A 80: 4639-43 PubMed EcoliWiki page
- ↑ Smith DW et al. (1985) Importance of state of methylation of oriC GATC sites in initiation of DNA replication in Escherichia coli. EMBO J 4: 1319-26 PubMed EcoliWiki page
- ↑ Messer W et al. (1985) Effect of dam methylation on the activity of the E. coli replication origin, oriC. EMBO J 4: 1327-32 PubMed EcoliWiki page
- ↑ 12.0 12.1 12.2 12.3 12.4 12.5 12.6 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
- ↑ 13.00 13.01 13.02 13.03 13.04 13.05 13.06 13.07 13.08 13.09 13.10 13.11 13.12 13.13 13.14 13.15 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
