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ddlA:Gene Product(s)
From EcoliWiki
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
DdlA |
|---|---|
| Synonyms | |
| Product description |
D-alanine:D-alanine ligase A, ADP-forming[3] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0005524 |
ATP binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0009252 |
peptidoglycan biosynthetic process |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000291 |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |
| GO:0005618 |
cell wall |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc [5] |
complete | |||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0007047 |
cell wall organization |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [5] |
complete | |||
| GO:0008360 |
regulation of cell shape |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [5] |
complete | |||
| GO:0008716 |
D-alanine-D-alanine ligase activity |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000291 |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |
| GO:0016874 |
ligase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [5] |
complete | |||
| GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [5] |
complete | |||
| GO:0000287 |
magnesium ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [5] |
complete | |||
| GO:0030145 |
manganese ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [5] |
complete | |||
| GO:0046872 |
metal ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [5] |
complete | |||
| GO:0008716 |
D-alanine-D-alanine ligase activity |
IDA: Inferred from Direct Assay |
F |
Activity of D-alanyl-D-alanine ligase was determined in a coupled assay using purified DdlA enzyme (Table 1). Enzyme activity inhibition was also shown with addition of known D-alanyl-D-alanine ligase inhibitor, heptyl phosphinate (I) in Figure 3. |
required field missing | |||
| GO:0008716 |
D-alanine-D-alanine ligase activity |
IDA: Inferred from Direct Assay |
F |
Table 3 shows the results of kinetic experiments, including Kcat, for assays performed on purified DdlA. |
required field missing | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Experiment(s):EBI-879997 | |||
|
Protein |
Experiment(s):EBI-879997 | |||
|
Protein |
Experiment(s):EBI-879997 | |||
|
Protein |
Experiment(s):EBI-885024 | |||
|
Protein |
Experiment(s):EBI-885024 | |||
|
Protein |
Experiment(s):EBI-885024 | |||
|
Protein |
Experiment(s):EBI-885024 | |||
|
Protein |
Experiment(s):EBI-885024 | |||
|
Protein |
Experiment(s):EBI-1136403 | |||
|
Protein |
Experiment(s):EBI-1136403 | |||
|
Protein |
Experiment(s):EBI-1136403 | |||
|
Protein |
Experiment(s):EBI-1136403 | |||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Source | Notes |
|---|---|---|---|---|
|
cytoplasm |
From EcoCyc[10] |
| ||
| edit table |
Notes
Structure and Physical Properties
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MEKLRVGIVF GGKSAEHEVS LQSAKNIVDA IDKSRFDVVL LGIDKQGQWH VSDASNYLLN ADDPAHIALR PSATSLAQVP GKHEHQLIDA QNGQPLPTVD VIFPIVHGTL GEDGSLQGML RVANLPFVGS DVLASAACMD KDVTKRLLRD AGLNIAPFIT LTRANRHNIS FAEVESKLGL PLFVKPANQG SSVGVSKVTS EEQYAIAVDL AFEFDHKVIV EQGIKGREIE CAVLGNDNPQ ASTCGEIVLT SDFYAYDTKY IDEDGAKVVV PAAIAPEIND KIRAIAVQAY QTLGCAGMAR VDVFLTPENE VVINEINTLP GFTNISMYPK LWQASGLGYT DLITRLIELA LERHAADNAL KTTM |
|---|---|
| Length |
364 |
| Mol. Wt |
39.315 kDa |
| pI |
5.0 (calculated) |
| Extinction coefficient |
22,920 - 23,420 (calc based on 8 Y, 2 W, and 4 C residues) |
| edit table |
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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Structure
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Structure figures
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| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 4.0 4.1 4.2 4.3 4.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
- ↑ al-Bar OA et al. (1992) D-alanine: D-alanine ligase of Escherichia coli. Expression, purification and inhibitory studies on the cloned enzyme. Biochem J 282 ( Pt 3): 747-52 PubMed EcoliWiki page
- ↑ Zawadzke LE et al. (1991) Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes. Biochemistry 30: 1673-82 PubMed EcoliWiki page
- ↑ 8.0 8.1 8.2 8.3 8.4 8.5 8.6 8.7 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
- ↑ 9.0 9.1 9.2 9.3 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
