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dipZ:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dsbD

Mnemonic

Disulfide bond

Synonyms

dipZ, ECK4130, b4136, JW5734, cutA2, cycZ, htrI, dsbD[1][2]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

94 minutes, 94 minutes 

MG1655: 4363065..4361368
gbrowse_img.php?coord=4361368..4363065&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 4369720..4368023
gbrowse_img.php?coord=4368023..4369720&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4463427..4461730
gbrowse_img.php?coord=4461730..4463427&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

REL606

NC_012967: 4343763..4342066
gbrowse_img.php?coord=4342066..4343763&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 4300103..4301800
gbrowse_img.php?coord=4300103..4301800&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔdipZ762::kan

PMID:16738554[3]

CGSC:100610

dipZ4::Cm

PMID:8917542[4] PMID:9352906[5]

CGSC:65873

ΔdsbD

PMID:9335267[6], PMID:10545108[7]

This strain contains an internal in-frame deletion of 536 codons of the dsbD gene. We replaced the deleted DNA sequences by an EcoRI site,

Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

Plasmid clone

Shigen

PMID:16769691[8]

Status:

Primer 1:

Primer 2:

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12178

EchoBASE (EcoliWiki Page)

EchoBASE:EB2095

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4136

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5218

EcoGene (EcoliWiki Page)

EcoGene:EG12178

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:DSBD

CGSC (EcoliWiki Page)

CGSC:34213

ASAP (EcoliWikiPage)

ASAP:ABE-0013539


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  4. Rietsch A et al. (1996) An in vivo pathway for disulfide bond isomerization in Escherichia coli. Proc Natl Acad Sci U S A 93: 13048-53 PubMed EcoliWiki page
  5. Rietsch A et al. (1997) Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin. J Bacteriol 179: 6602-8 PubMed EcoliWiki page
  6. Link AJ et al. (1997) Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J Bacteriol 179: 6228-37 PubMed EcoliWiki page
  7. Stewart EJ et al. (1999) Six conserved cysteines of the membrane protein DsbD are required for the transfer of electrons from the cytoplasm to the periplasm of Escherichia coli. EMBO J 18: 5963-71 PubMed EcoliWiki page
  8. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page

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