EcoliHub logo

Try the new EcoliHub and let us know what you'd like to see.

djlA:On One Page

From EcoliWiki

Jump to: navigation, search
Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]



See Help:Quickview for help with entering information in the Quickview table.

Standard Name

djlA

Gene Synonym(s)

ECK0056, b0055, JW0054, yabH[1]

Product Desc.

chaperone with DnaK[2][3]

DnaK co-chaperone; membrane-asssociated protein with single transmembrane domain and a C-terminal J-like domain; mutants rapidly accumulate suppressors[4]

Product Synonyms(s)

DnaJ-like protein, membrane anchored[1], B0055[2][1], YabH[2][1], DjlA[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): djlA[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(djlA)    Textpresso(djlA)

edit table

See Help:Quickview for help with entering information in the Quickview table.

Notes

[back to top]


DjlA overproduction activates RcsBC and Cps synthesis and causes mucoidy. Normal levels of DjlA act as an Rcs negative regulator. The DjlA transmembrane domain is a dimerization domain.[4]



Gene

[back to top]


Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

djlA

Mnemonic

DnaJ-like

Synonyms

ECK0056, b0055, JW0054, yabH[1]

edit table

Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

1.24 minutes 

MG1655: 57364..58179
gbrowse_img.php?coord=57364..58179&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 61559..62374
gbrowse_img.php?coord=61559..62374&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 57364..58179
gbrowse_img.php?coord=57364..58179&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 57364..58179
gbrowse_img.php?coord=57364..58179&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 57364..58179
gbrowse_img.php?coord=57364..58179&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

edit table

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
edit table

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

djlAL15R

L15R

Loss of activation of rcs

seeded from UniProt:P31680

djlAM16R

M16R

Only partial activation of rcs

seeded from UniProt:P31680

djlAH233Q

H233Q

Loss of activity

seeded from UniProt:P31680

ΔdjlA (Keio:JW0054)

deletion

deletion

PMID:16738554

Shigen
CGSC8363[5]

ΔdjlA766::kan

PMID:16738554

CGSC:100612


edit table

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0054

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCAGTATTGGGGAAAAATCAT

Primer 2:CCTTTAAACCCTTTCTGCTGCTT

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 39% [6]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[5]

est. P1 cotransduction: 37% [6]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

edit table

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11570

EchoBASE (EcoliWiki Page)

EchoBASE:EB1530

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0055

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0068

EcoGene (EcoliWiki Page)

EcoGene:EG11570

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YABH

ASAP (EcoliWikiPage)

ASAP:ABE-0000187


edit table

Notes

Links

[back to top]



Product(s)

[back to top]



Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

DjlA

Synonyms

DnaJ-like protein, membrane anchored[1], B0055[2][1], YabH[2][1], DjlA[2][1]

Product description

chaperone with DnaK[2][3]

DnaK co-chaperone; membrane-asssociated protein with single transmembrane domain and a C-terminal J-like domain; mutants rapidly accumulate suppressors[4]

EC number (for enzymes)


edit table

Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0031072

heat shock protein binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001623

F

Seeded from EcoCyc [7]

complete

GO:0030188

chaperone regulator activity

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01153

F

Seeded from EcoCyc [7]

complete

GO:0016021

integral to membrane

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc [7]

complete

GO:0016020

membrane

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0006457

protein folding

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005886

plasma membrane

GO_REF:0000020
GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

HAMAP:MF_01153
SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [7]

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence
edit table

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

plasma membrane


edit table

Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MQYWGKIIGV AVALLMGGGF WGVVLGLLIG HMFDKARSRK MAWFANQRER QALFFATTFE
VMGHLTKSKG RVTEADIHIA SQLMDRMNLH GASRTAAQNA FRVGKSDNYP LREKMRQFRS
VCFGRFDLIR MFLEIQIQAA FADGSLHPNE RAVLYVIAEE LGISRAQFDQ FLRMMQGGAQ
FGGGYQQQTG GGNWQQAQRG PTLEDACNVL GVKPTDDATT IKRAYRKLMS EHHPDKLVAK
GLPPEMMEMA KQKAQEIQQA YELIKQQKGF K
Length

271

Mol. Wt

30.579 kDa

pI

10.1 (calculated)

Extinction coefficient

30,940 - 31,190 (calc based on 6 Y, 4 W, and 2 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
edit table


Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11570-MONOMER

UniProt (EcoliWiki Page)

UniProt:P31680

PFAM (EcoliWiki Page)

PFAM:PF00226

RefSeq (EcoliWiki Page)

RefSeq:NP_414597


edit table

Notes

Links

[back to top]




Expression

[back to top]



Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]


See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

djlA

edit table
Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


gbrowse_img.php?coord=57344..57384&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
edit table


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0055 (EcoliWiki Page)

NCBI GEO profiles for djlA

microarray

GenExpDB:b0055 (EcoliWiki Page)

Summary of data for djlA from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
edit table

Notes

Accessions Related to djlA Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11570

EchoBASE (EcoliWiki Page)

EchoBASE:EB1530

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0055

EcoGene (EcoliWiki Page)

EcoGene:EG11570


edit table

Notes

Links

[back to top]



Evolution

[back to top]



Homologs in Other Organisms

[back to top]


See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000014657 (score: 1.000; bootstrap: 100%)
  • ENSANGP00000019419 (score: 0.078)
  • ENSANGP00000010200 (score: 0.063)

From Inparanoid:20070104

Shigella flexneri

YABH

From SHIGELLACYC

E. coli O157

YABH

From ECOO157CYC

edit table


Do-It-Yourself Web Tools

Notes

Families

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00226


edit table

Notes

Links

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

Categories

[back to top]


Retrieved from "http://ecoliwiki.net/colipedia/index.php/djlA:On_One_Page"
Personal tools