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dnaE:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaE

Mnemonic

DNA

Synonyms

ECK0183, b0184, JW0179, polC, sdgC[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

4.42 minutes 

MG1655: 205126..208608
gbrowse_img.php?coord=205126..208608&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 207968..211450
gbrowse_img.php?coord=207968..211450&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 205126..208608
gbrowse_img.php?coord=205126..208608&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 179230..182712
gbrowse_img.php?coord=179230..182712&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 205125..208607
gbrowse_img.php?coord=205125..208607&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

dnaE1026(ts)

temperature sensitive

CGSC:8012

dnaE486(ts)

temperature sensitive

CGSC:5641

dnaE1044

CGSC:73004

dnaE1061

CGSC:73007

dnaE1126

CGSC:73010

dnaE74(ts)

temperature sensitive

PMID:3158798[2] PMID:15028700[3]

CGSC:85748


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0179

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCTCTGAACCACGTTTCGTACA

Primer 2:CCGTCAAACTCCAGTTCCACCTG

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 6% [6]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[5]

est. P1 cotransduction: 36% [6]
Synonyms:zae-502::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10238

EchoBASE (EcoliWiki Page)

EchoBASE:EB0234

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0184

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0221

EcoGene (EcoliWiki Page)

EcoGene:EG10238

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:DNAE

CGSC (EcoliWiki Page)

CGSC:373

ASAP (EcoliWikiPage)

ASAP:ABE-0000624


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Brotcorne-Lannoye A et al. (1985) Involvement of DNA polymerase III in UV-induced mutagenesis of bacteriophage lambda. Mol Gen Genet 199: 64-9 PubMed EcoliWiki page
  3. Strauss B et al. (2004) Cell death in Escherichia coli dnaE(Ts) mutants incubated at a nonpermissive temperature is prevented by mutation in the cydA gene. J Bacteriol 186: 2147-55 PubMed EcoliWiki page
  4. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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