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dnaJ:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaJ

Mnemonic

DNA

Synonyms

ECK0015, b0015, JW0014, groP, grpC[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

0.31 minutes 

MG1655: 14168..15298
gbrowse_img.php?coord=14168..15298&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 14166..15296
gbrowse_img.php?coord=14166..15296&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 14168..15298
gbrowse_img.php?coord=14168..15298&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 14168..15298
gbrowse_img.php?coord=14168..15298&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 14168..15298
gbrowse_img.php?coord=14168..15298&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

dnaJC144S

C144S

Loss of dnaK-independent chaperone activity; when associated with S-147; S- 197 and S-200

seeded from UniProt:P08622

dnaJC161H

C161H

No effect on chaperone function; when associated with H-183

seeded from UniProt:P08622

dnaJC147S

C147S

Loss of dnaK-independent chaperone activity; when associated with S-144; S- 197 and S-200

seeded from UniProt:P08622

dnaJKE51AA

KE51AA

No effect

seeded from UniProt:P08622

dnaJY54A

Y54A

No effect

seeded from UniProt:P08622

dnaJE55A

E55A

No effect

seeded from UniProt:P08622

dnaJTD58AA

TD58AA

No effect

seeded from UniProt:P08622

dnaJDQ67AA

DQ67AA

No effect

seeded from UniProt:P08622

dnaJSQ60AA

SQ60AA

No effect

seeded from UniProt:P08622

dnaJKR62AA

KR62AA

No effect

seeded from UniProt:P08622

dnaJKE48AA

KE48AA

No effect

seeded from UniProt:P08622

dnaJKE41AA

KE41AA

No effect

seeded from UniProt:P08622

dnaJE44A

E44A

No effect

seeded from UniProt:P08622

dnaJK46A

K46A

No effect

seeded from UniProt:P08622

dnaJF47A

F47A

Loss of function

seeded from UniProt:P08622

dnaJMK30AA

MK30AA

No effect

seeded from UniProt:P08622

dnaJY32A

Y32A

No effect

seeded from UniProt:P08622

dnaJH33Q

H33Q

Loss of ability to stimulate dnaK ATPase activity

seeded from UniProt:P08622

dnaJQ38A

Q38A

No effect

seeded from UniProt:P08622

dnaJP34F

P34F

Loss of function

seeded from UniProt:P08622

dnaJD35N

D35N

Loss of ability to bind dnaK

seeded from UniProt:P08622

dnaJR36A

R36A

Decrease in chaperone function

seeded from UniProt:P08622

dnaJN37A

N37A

Decrease in chaperone function

seeded from UniProt:P08622

dnaJL28A

L28A

No effect

seeded from UniProt:P08622

dnaJRE19AA

RE19AA

No effect

seeded from UniProt:P08622

dnaJY25A

Y25A

Loss of activity

seeded from UniProt:P08622

dnaJK26A

K26A

Loss of activity

seeded from UniProt:P08622

dnaJA29G

A29G

No effect

seeded from UniProt:P08622

dnaJR27A

R27A

No effect

seeded from UniProt:P08622

ΔdnaJ (Keio:JW0014)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8343[3]

dnaJC161S

C161S

Loss of function; when associated with S-164; S-183 and S-186

seeded from UniProt:P08622

dnaJC164H

C164H

No effect on chaperone function; when associated with H-183

seeded from UniProt:P08622

dnaJC164S

C164S

Loss of function; when associated with S-161; S-183 and S-186

seeded from UniProt:P08622

dnaJC186H

C186H

No effect on chaperone function

seeded from UniProt:P08622

dnaJC186S

C186S

Loss of function; when associated with S-161; S-164 and S-184

seeded from UniProt:P08622

dnaJC197S

C197S

Loss of dnaK-independent chaperone activity; when associated with S-144; S- 147 and S-200

seeded from UniProt:P08622

dnaJC183H

C183H

No effect on chaperone function. Same effect; when associated with H-161 or H-164

seeded from UniProt:P08622

dnaJC183S

C183S

Loss of function; when associated with S-161; S-164 and S-186

seeded from UniProt:P08622

dnaJC200S

C200S

Loss of dnaK-independent chaperone activity; when associated with S-144; S- 147 and S-197

seeded from UniProt:P08622

dnaJ259(ts)

temperature sensitive

CGSC:6712

ΔdnaJ735::kan

PMID:16738554[2]

CGSC:100626

ΔdnaJ

  • defective mini-Tn10 insertion
  • block λ DNA replication at all temp
  • growth defect above 30˚C

PMID:2144273[4]

Strain BW2952

T5C

A2V

Nonsynonomous mutation

PMID:19376874[5]

This is a difference relative to E. coli K-12 MG1655.

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0014

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCTAAGCAAGATTATTACGA

Primer 2:CCGCGGGTCAGGTCGTCAAAAAA

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[3]

est. P1 cotransduction: 61% [7]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[3]

est. P1 cotransduction: 52% [7]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10240

EchoBASE (EcoliWiki Page)

EchoBASE:EB0236

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0015

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0020

EcoGene (EcoliWiki Page)

EcoGene:EG10240

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:DNAJ

CGSC (EcoliWiki Page)

CGSC:845

ASAP (EcoliWikiPage)

ASAP:ABE-0000054


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Sell SM et al. (1990) Isolation and characterization of dnaJ null mutants of Escherichia coli. J Bacteriol 172: 4827-35 PubMed EcoliWiki page
  5. ↑ Ferenci T et al. (2009) Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12. J Bacteriol 191: 4025-9 PubMed EcoliWiki page
  6. ↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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