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dut:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dut

Mnemonic

dUTPase

Synonyms

ECK3630, b3640, JW3615, dnaS, sof[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

82.16 minutes 

MG1655: 3811955..3812410
gbrowse_img.php?coord=3811955..3812410&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3752287..3752742
gbrowse_img.php?coord=3752287..3752742&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3826483..3826028
gbrowse_img.php?coord=3826028..3826483&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3909532..3909987
gbrowse_img.php?coord=3909532..3909987&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 3700281..3700736
gbrowse_img.php?coord=3700281..3700736&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

dutD89N

D89N

Reduces catalytic activity 40000- fold

seeded from UniProt:P06968

dut-1

PMID:148458[2]

CGSC:6840


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW3615

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCAAAAAAATCGACGTTAAGAT

Primer 2:CCtTGACGACCAGAGTGACCAAA

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[4]

est. P1 cotransduction: % [5]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[4]

est. P1 cotransduction: 57% [5]

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10251

EchoBASE (EcoliWiki Page)

EchoBASE:EB0247

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3640

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4464

EcoGene (EcoliWiki Page)

EcoGene:EG10251

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:DUT

CGSC (EcoliWiki Page)

CGSC:832

ASAP (EcoliWikiPage)

ASAP:ABE-0011899


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Hochhauser SJ & Weiss B (1978) Escherichia coli mutants deficient in deoxyuridine triphosphatase. J Bacteriol 134: 157-66 PubMed EcoliWiki page
  3. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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