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emrY:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

emrY

Mnemonic

Efflux-multidrug resistance

Synonyms

ECK2363, b2367, JW2364[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

53.42 minutes 

MG1655: 2480198..2478660
gbrowse_img.php?coord=2478660..2480198&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2416403..2414865
gbrowse_img.php?coord=2414865..2416403&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2487622..2486084
gbrowse_img.php?coord=2486084..2487622&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2571963..2570425
gbrowse_img.php?coord=2570425..2571963&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2364465..2366003
gbrowse_img.php?coord=2364465..2366003&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔemrY (Keio:JW2364)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9888[3]

emrY

PMID:12519969[4]

ASAP

At position 956 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20778

ΔemrY776::kan

PMID:16738554[2]

CGSC:100708

Strain BW2952

T763A

L249Q

Nonsynonomous mutation

PMID:19376874[5]

This is a strain-specific difference in BW2952 relative to E. coli K-12 MG1655.

Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW2364

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCAATCACTAAATCAACTCC

Primer 2:CCgCCAACGCCTTTCGCTGTAAA

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[3]

est. P1 cotransduction: % [7]
Synonyms:zej-223::Tn10

zfd-1::Tn10

Linked marker

CAG18467 = CGSC7408[3]

est. P1 cotransduction: 64% [7]
Synonyms:zfb-1::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG13283

EchoBASE (EcoliWiki Page)

EchoBASE:EB3068

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2367

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2902

EcoGene (EcoliWiki Page)

EcoGene:EG13283

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:EMRY

ASAP (EcoliWikiPage)

ASAP:ABE-0007806


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Glasner JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res 31: 147-51 PubMed EcoliWiki page
  5. Ferenci T et al. (2009) Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12. J Bacteriol 191: 4025-9 PubMed EcoliWiki page
  6. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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