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endA:On One Page

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See Help:Quickview for help with entering information in the Quickview table.

Standard Name

endA

Gene Synonym(s)

ECK2940, b2945, JW2912[1]

Product Desc.

DNA-specific endonuclease I[2][3]

DNA-specific endonuclease I, periplasmic; inhibited by RNA; extensive DNA breakdown[4]

Product Synonyms(s)

DNA-specific endonuclease I[1], B2945[2][1], EndA[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): endA[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(endA)    Textpresso(endA)

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Notes

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First 22 aa are predicted to be a type I signal peptide.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

endA

Mnemonic

Endonuclease

Synonyms

ECK2940, b2945, JW2912[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

66.56 minutes 

MG1655: 3088369..3089076
gbrowse_img.php?coord=3088369..3089076&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2976098..2976805
gbrowse_img.php?coord=2976098..2976805&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3089003..3089710
gbrowse_img.php?coord=3089003..3089710&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

BW2952

NC_012759: 2975517..2976224
gbrowse_img.php?coord=2975517..2976224&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

Strain DH10B

E208K

Nonsynonomous mutation

PMID:18245285

ASAP

This is a SNP in E. coli K-12 Strain DH10B

ΔendA (Keio:JW2912)

deletion

deletion

PMID:16738554

Shigen
CGSC10253[5]

endA1

CGSC:5430

endA2

CGSC:5609

endA101

CGSC:8416

endA5

CGSC:10668

endA3

CGSC:14266

endA4

CGSC:16230

endA6(del-ins)::kan

CGSC:35763

endA7(del-ins)::Cm

CGSC:35768

endA8(del:Frt:Tc)::Tc

PMID:7789817

CGSC:59095

endA9(del-ins)::FRT

CGSC:64683

ΔendA720::kan

PMID:16738554

CGSC:100710


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Notes

EndA is an enzyme in many proteobacteria. One function of the enzyme is cleaving unspecific internal sites at both RNA and DNA.[6] Cultures of mutant endA result in large amounts of chromosmal and plasmid DNA, which does not appear in the wild type allele. EndA is responsible for DNA and plasmid DNA decomposition [7].

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW2912

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTACCGTTATTTGTCTATTGC

Primer 2:CCGCTCTTTCGCGCCTGGCAAGC

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[5]

est. P1 cotransduction: 91% [8]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

nupG511::Tn10

Linked marker

CAG18472 = CGSC7433[5]

est. P1 cotransduction: 58% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11336

EchoBASE (EcoliWiki Page)

EchoBASE:EB1312

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2945

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3531

EcoGene (EcoliWiki Page)

EcoGene:EG11336

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ENDA

CGSC (EcoliWiki Page)

CGSC:824

ASAP (EcoliWikiPage)

ASAP:ABE-0009661


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

EndA

Synonyms

DNA-specific endonuclease I[1], B2945[2][1], EndA[2][1]

Product description

DNA-specific endonuclease I[2][3]

DNA-specific endonuclease I, periplasmic; inhibited by RNA; extensive DNA breakdown[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0042597

periplasmic space

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc 12.5 [9]

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc [10]

complete

GO:0004530

deoxyribonuclease I activity

GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.1.21.1

F

Seeded from EcoCyc [10]

complete

GO:0004519

endonuclease activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0255

F

Seeded from EcoCyc [10]

complete

GO:0004518

nuclease activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR007346
SP_KW:KW-0540

F

Seeded from EcoCyc [10]

complete

GO:0030288

outer membrane-bounded periplasmic space

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0006308

DNA catabolic process

P

Seeded from Riley et al 2006 [1].

required fields missing

GO:0000737

DNA catabolic process, endonucleolytic

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

periplasm

Periplasm

PMID:7867949

EchoLocation:endA


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MYRYLSIAAV VLSAAFSGPA LAEGINSFSQ AKAAAVKVHA DAPGTFYCGC KINWQGKKGV
VDLQSCGYQV RKNENRASRV EWEHVVPAWQ FGHQRQCWQD GGRKNCAKDP VYRKMESDMH
NLQPSVGEVN GDRGNFMYSQ WNGGEGQYGQ CAMKVDFKEK AAEPPARARG AIARTYFYMR
DQYNLTLSRQ QTQLFNAWNK MYPVTDWECE RDERIAKVQG NHNPYVQRAC QARKS
Length

235

Mol. Wt

26.707 kDa

pI

9.4 (calculated)

Extinction coefficient

56,380 - 57,380 (calc based on 12 Y, 7 W, and 8 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

motif

1-22

UniProt Manual:Signal Peptides

UniProt:P25736


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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11336-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P25736

UniProt (EcoliWiki Page)

UniProt:P25736

PFAM (EcoliWiki Page)

PFAM:PF04231

RefSeq (EcoliWiki Page)

RefSeq:NP_417420


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

endA

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Figure courtesy of RegulonDB

Notes

Translation and Regulation of Translation

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gbrowse_img.php?coord=3088349..3088389&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b2945 (EcoliWiki Page)

NCBI GEO profiles for endA

microarray

GenExpDB:b2945 (EcoliWiki Page)

Summary of data for endA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to endA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11336

EchoBASE (EcoliWiki Page)

EchoBASE:EB1312

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2945

EcoGene (EcoliWiki Page)

EcoGene:EG11336


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Shigella flexneri

ENDA

From SHIGELLACYC

E. coli O157

ENDA

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF04231


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Altermark B et al. (2007) Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae. FEBS J 274: 252-63 PubMed EcoliWiki page
  7. ↑ Lin JJ (1992) Endonuclease A degrades chromosomal and plasmid DNA of Escherichia coli present in most preparations of single stranded DNA from phagemids. Proc Natl Sci Counc Repub China B 16: 1-5 PubMed EcoliWiki page
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  9. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  10. ↑ 10.0 10.1 10.2 10.3 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70

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