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Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

fliF

Gene Synonym(s)

ECK1936, b1938, JW1922, flaBI, flaAII 1, flaAII.1, AII.1, flaAII, 1, flaAll.1[1][2]

Product Desc.

flagellar M-ring protein FliF; basal-body MS(membrane and supramembrane)-ring and collar protein[2][3];

Component of Flagellar Motor Complex[2][3]; Flagellum[3]

Flagellar basal body M-ring protein[4]

Product Synonyms(s)

flagellar basal-body MS-ring and collar protein[1], B1938[2][1], FliF[2][1]

Function from GO

Molecular Function

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): fliFGHIJK[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(fliF)    Textpresso(fliF)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fliF

Mnemonic

Flagella

Synonyms

ECK1936, b1938, JW1922, flaBI, flaAII 1, flaAII.1, AII.1, flaAII, 1, flaAll.1[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

43.35 minutes, 43.35 minutes 

MG1655: 2011253..2012911
gbrowse_img.php?coord=2011253..2012911&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2015366..2017024
gbrowse_img.php?coord=2015366..2017024&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2102261..2103919
gbrowse_img.php?coord=2102261..2103919&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 1903736..1905394
gbrowse_img.php?coord=1903736..1905394&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔfliF (Keio:JW1922)

deletion

deletion

PMID:16738554

Shigen
CGSC9598[5]

fliF

PMID:12519969

ASAP

At position 820 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20538

ΔfliF784::kan

PMID:16738554

CGSC:100936


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW1922

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAATGCGACTGCAGCCCAGAC

Primer 2:CCtTCATGATCGTTATTTATCCA

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[5]

est. P1 cotransduction: 47% [6]

zed-3069::Tn10

Linked marker

CAG18451 = CGSC7395[5]

est. P1 cotransduction: 38% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11347

EchoBASE (EcoliWiki Page)

EchoBASE:EB1323

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1938

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2354

EcoGene (EcoliWiki Page)

EcoGene:EG11347

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:FLIF

CGSC (EcoliWiki Page)

CGSC:17707

ASAP (EcoliWikiPage)

ASAP:ABE-0006454


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FliF

Synonyms

flagellar basal-body MS-ring and collar protein[1], B1938[2][1], FliF[2][1]

Product description

flagellar M-ring protein FliF; basal-body MS(membrane and supramembrane)-ring and collar protein[2][3];

Component of Flagellar Motor Complex[2][3]; Flagellum[3]

Flagellar basal body M-ring protein[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0019861

flagellum

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0282

C

Seeded from EcoCyc 12.5 [7]

complete

GO:0016021

integral to membrane

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc [8]

complete

GO:0016020

membrane

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [7]

complete

GO:0009431

flagellin-based flagellum basal body, MS ring

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000067

C

Seeded from EcoCyc [8]

complete

GO:0003774

motor activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000067

F

Seeded from EcoCyc [8]

complete

GO:0001539

ciliary or flagellar motility

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000067

P

Seeded from EcoCyc [8]

complete

GO:0005515

protein binding

PMID:16606699

IPI: Inferred from Physical Interaction

UniProtKB:P0AC41

F

Seeded from EcoCyc [8]

complete

GO:0009296

flagellum biogenesis

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005886

plasma membrane

GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [8]

complete

GO:0009288

bacterial-type flagellum

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0975

C

Seeded from EcoCyc [8]

complete

GO:0009425

bacterial-type flagellum basal body

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0142

C

Seeded from EcoCyc [8]

complete

GO:0042330

taxis

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of Flagellar Motor Complex

could be indirect

Protein

sdhA

PMID:16606699

Experiment(s):EBI-1141422

Protein

Subunits of Flagellum

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MNATAAQTKS LEWLNRLRAN PKIPLIVAGS AAVAVMVALI LWAKAPDYRT LFSNLSDQDG
GAIVSQLTQM NIPYRFSEAS GAIEVPADKV HELRLRLAQQ GLPKGGAVGF ELLDQEKFGI
SQFSEQVNYQ RALEGELSRT IETIGPVKGA RVHLAMPKPS LFVREQKSPS ASVTVNLLPG
RALDEGQISA IVHLVSSAVA GLPPGNVTLV DQGGHLLTQS NTSGRDLNDA QLKYASDVEG
RIQRRIEAIL SPIVGNGNIH AQVTAQLDFA SKEQTEEQYR PNGDESHAAL RSRQLNESEQ
SGSGYPGGVP GALSNQPAPA NNAPISTPPA NQNNRQQQAS TTSNSGPRST QRNETSNYEV
DRTIRHTKMN VGDVQRLSVA VVVNYKTLPD GKPLPLSNEQ MKQIEDLTRE AMGFSEKRGD
SLNVVNSPFN SSDESGGELP FWQQQAFIDQ LLAAGRWLLV LLVAWLLWRK AVRPQLTRRA
EAMKAVQQQA QAREEVEDAV EVRLSKDEQL QQRRANQRLG AEVMSQRIRE MSDNDPRVVA
LVIRQWINND HE
Length

552

Mol. Wt

60.588 kDa

pI

7.0 (calculated)

Extinction coefficient

50,420 (calc based on 8 Y, 7 W, and C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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tmhmm.php?gene=fliF&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:FLIF-FLAGELLAR-MS-RING

UniProt (EcoliWiki Page)

UniProt:P25798

PFAM (EcoliWiki Page)

PFAM:PF01514

RefSeq (EcoliWiki Page)

RefSeq:NP_416448


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

fliFGHIJK

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2011233..2011273&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b1938 (EcoliWiki Page)

NCBI GEO profiles for fliF

microarray

GenExpDB:b1938 (EcoliWiki Page)

Summary of data for fliF from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (2010949..2011302) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:A6[9]

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Notes

Accessions Related to fliF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11347

EchoBASE (EcoliWiki Page)

EchoBASE:EB1323

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1938

EcoGene (EcoliWiki Page)

EcoGene:EG11347


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Notes

Links

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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF01514


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 8.6 8.7 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

Categories

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