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folA:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

folA

Mnemonic

Folate

Synonyms

ECK0049, b0048, JW0047, tmrA, tmr[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

1.07 minutes 

MG1655: 49823..50302
gbrowse_img.php?coord=49823..50302&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 54018..54497
gbrowse_img.php?coord=54018..54497&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 49823..50302
gbrowse_img.php?coord=49823..50302&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 49823..50302
gbrowse_img.php?coord=49823..50302&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 49823..50302
gbrowse_img.php?coord=49823..50302&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

folAL28R

L28R

(in strain: B[RT500] isozyme 2)

Strain variation; seeded from UniProt:P0ABQ4

folAW30G

W30G

(in strain: 1810)

Strain variation; seeded from UniProt:P0ABQ4

folAE154K

E154K

(in strain: B[MB1428])

Strain variation; seeded from UniProt:P0ABQ4

folAE154Q

E154Q

(in strain: 1810)

Strain variation; seeded from UniProt:P0ABQ4

ΔfolA207::kan

PMID:3290203[2]

CGSC:79051

ΔfolA201::kan

CGSC:39881


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0047

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCATCAGTCTGATTGCGGCGTT

Primer 2:CCCCGCCGCTCCAGAATCTCAAA

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[4]

est. P1 cotransduction: 54% [5]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[4]

est. P1 cotransduction: 26% [5]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10326

EchoBASE (EcoliWiki Page)

EchoBASE:EB0322

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0048

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0058

EcoGene (EcoliWiki Page)

EcoGene:EG10326

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z0055

CGSC (EcoliWiki Page)

CGSC:744

ASAP (EcoliWikiPage)

ASAP:ABE-0000164


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Ahrweiler PM & Frieden C (1988) Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments. J Bacteriol 170: 3301-4 PubMed EcoliWiki page
  3. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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