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Standard Name

folA

Gene Synonym(s)

ECK0049, b0048, JW0047, tmrA, tmr[1]

Product Desc.

dihydrofolate reductase[2][3]

Dihydrofolate reductase; trimethoprim resistance[4]

Product Synonyms(s)

dihydrofolate reductase[1], B0048[2][1], Tmr[2][1], TmrA[2][1], FolA[2][1]

Function from GO

Biological Process

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): folA[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(folA)    Textpresso(folA)

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Notes

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folA is an essential gene. TyrR regulon.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

folA

Mnemonic

Folate

Synonyms

ECK0049, b0048, JW0047, tmrA, tmr[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

1.07 minutes 

MG1655: 49823..50302
gbrowse_img.php?coord=49823..50302&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 54018..54497
gbrowse_img.php?coord=54018..54497&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 49823..50302
gbrowse_img.php?coord=49823..50302&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 49823..50302
gbrowse_img.php?coord=49823..50302&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 49823..50302
gbrowse_img.php?coord=49823..50302&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

folAL28R

L28R

(in strain: B[RT500] isozyme 2)

Strain variation; seeded from UniProt:P0ABQ4

folAW30G

W30G

(in strain: 1810)

Strain variation; seeded from UniProt:P0ABQ4

folAE154K

E154K

(in strain: B[MB1428])

Strain variation; seeded from UniProt:P0ABQ4

folAE154Q

E154Q

(in strain: 1810)

Strain variation; seeded from UniProt:P0ABQ4

ΔfolA207::kan

PMID:3290203

CGSC:79051

ΔfolA201::kan

CGSC:39881


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0047

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATCAGTCTGATTGCGGCGTT

Primer 2:CCCCGCCGCTCCAGAATCTCAAA

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 54% [6]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[5]

est. P1 cotransduction: 26% [6]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10326

EchoBASE (EcoliWiki Page)

EchoBASE:EB0322

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0048

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0058

EcoGene (EcoliWiki Page)

EcoGene:EG10326

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z0055

CGSC (EcoliWiki Page)

CGSC:744

ASAP (EcoliWikiPage)

ASAP:ABE-0000164


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FolA

Synonyms

dihydrofolate reductase[1], B0048[2][1], Tmr[2][1], TmrA[2][1], FolA[2][1]

Product description

dihydrofolate reductase[2][3]

Dihydrofolate reductase; trimethoprim resistance[4]

EC number (for enzymes)

1.5.1.3[1]

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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0050661

NADP or NADPH binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012259

F

Seeded from EcoCyc [7]

complete

GO:0046677

response to antibiotic

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0046
SP_KW:KW-0817

P

Seeded from EcoCyc [7]

complete

GO:0031427

response to methotrexate

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0487

P

Seeded from EcoCyc [7]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc [7]

complete

GO:0009165

nucleotide biosynthetic process

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001796
InterPro:IPR012259

P

Seeded from EcoCyc [7]

complete

GO:0006730

one-carbon metabolic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0554

P

Seeded from EcoCyc [7]

complete

GO:0006545

glycine biosynthetic process

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001796
InterPro:IPR012259

P

Seeded from EcoCyc [7]

complete

GO:0004146

dihydrofolate reductase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR001796
InterPro:IPR012259
EC:1.5.1.3

F

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0055114

oxidation reduction

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc [7]

complete

GO:0046656

folic acid biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required field missing

GO:0009257

10-formyltetrahydrofolate biosynthetic process

PMID:6444603


IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 11.1[3].

Table 2 shows the results of assays performed using dihydrofolate as a substrate for the synthesis of tetrahydrofolate in the presence of NADPH.

complete

GO:0042493

response to drug

PMID:6444603

IDA: Inferred from Direct Assay

P

Trimethoprim

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

lrp

PMID:16606699

Experiment(s):EBI-1135444

Protein

sdhA

PMID:16606699

Experiment(s):EBI-1135444

Protein

yeaG

PMID:16606699

Experiment(s):EBI-1135444

Protein

thrS

PMID:16606699

Experiment(s):EBI-1135444

Protein

mdoB

PMID:15690043

Experiment(s):EBI-891656

Protein

proS

PMID:15690043

Experiment(s):EBI-891656

Protein

rplM

PMID:15690043

Experiment(s):EBI-891656

Protein

ybhC

PMID:15690043

Experiment(s):EBI-891656

Protein

ssuD

PMID:15690043

Experiment(s):EBI-891656

Protein

hsrA

PMID:15690043

Experiment(s):EBI-891656

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MISLIAALAV DRVIGMENAM PWNLPADLAW FKRNTLNKPV IMGRHTWESI GRPLPGRKNI
ILSSQPGTDD RVTWVKSVDE AIAACGDVPE IMVIGGGRVY EQFLPKAQKL YLTHIDAEVE
GDTHFPDYEP DDWESVFSEF HDADAQNSHS YCFEILERR
Length

159

Mol. Wt

17.999 kDa

pI

4.7 (calculated)

Extinction coefficient

33,460 - 33,710 (calc based on 4 Y, 5 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
  • Representative Escherichia coli structure:
    1DDS|A
    1-159 of 159 residues (E-value: 2e-86) (Percent Identity: 100.00)
  • Representative homolog:
    4DFR|A (Escherichia coli B)
    1-159 of 159 residues (E-value: 2e-85) (Percent Identity: 98.74)
  • View the PDB Table for folA
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:DIHYDROFOLATEREDUCT-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0ABQ4

PFAM (EcoliWiki Page)

PFAM:PF00186

RefSeq (EcoliWiki Page)

RefSeq:NP_414590


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

folA

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=49803..49843&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0048 (EcoliWiki Page)

NCBI GEO profiles for folA

microarray

GenExpDB:b0048 (EcoliWiki Page)

Summary of data for folA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (49562..49899) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ07; Well:E11[8]

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Notes

Accessions Related to folA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10326

EchoBASE (EcoliWiki Page)

EchoBASE:EB0322

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0048

EcoGene (EcoliWiki Page)

EcoGene:EG10326


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000000349 (score: 1.000; bootstrap: 74%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000029347 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00033316 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00007974 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000005901 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000012960 (score: 0.813)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000005566 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-010406-5 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040625-31 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0230113 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0004087 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA13324-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000025076 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000343033 (score: 1.000; bootstrap: 100%)
  • ENSP00000302637 (score: 1.000; bootstrap: 100%)
  • ENSP00000319170 (score: 0.882)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000012897 (score: 1.000; bootstrap: 100%)
  • ENSMMUP00000024036 (score: 0.539)

From Inparanoid:20070104

Mus musculus

  • MGI:94890 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000029175 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000026122 (score: 0.602)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000018259 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YOR236W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000135205 (score: 1.000; bootstrap: 100%)
  • NEWSINFRUP00000134686 (score: 0.166)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00007900001 (score: 1.000; bootstrap: 100%)
  • GSTENP00015908001 (score: 0.140)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000049617 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

FOLA

From SHIGELLACYC

E. coli O157

Z0055

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00186


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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