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gadB:Gene Product(s)
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
GadB |
|---|---|
| Synonyms |
glutamate decarboxylase B, PLP-dependent[1], B1493[2][1], GadB[2][1] |
| Product description |
glutamate decarboxylase B subunit[2][3]; Component of glutamate decarboxylase B[2][3] Glutamate decarboxylase B, vitamin B6-dependent; hexameric[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004351 |
glutamate decarboxylase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc [8] |
complete | |||
| GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc [8] |
complete | |||
| GO:0051454 |
intracellular pH elevation |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc [8] |
complete | |||
| GO:0004351 |
glutamate decarboxylase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0006536 |
glutamate metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [8] |
complete | |||
| GO:0016020 |
membrane |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0019752 |
carboxylic acid metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [8] |
complete | |||
| GO:0030170 |
pyridoxal phosphate binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [8] |
complete | |||
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [8] |
complete | |||
| GO:0005739 |
mitochondrion |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc [8] |
complete | |||
| GO:0005829 |
cytosol |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc [8] |
complete | |||
| GO:0016829 |
lyase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [8] |
complete | |||
| GO:0016831 |
carboxy-lyase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [8] |
complete | |||
| GO:0009063 |
amino acid catabolic process |
P |
Seeded from EcoCyc 10.6[2]. |
required fields missing | ||||
| GO:0006520 |
amino acid metabolic process |
P |
Seeded from EcoCyc 10.6[2]. |
required fields missing | ||||
| GO:0051454 |
intracellular pH elevation |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc 11.1[3]. |
complete | |||
| GO:0004351 |
glutamate decarboxylase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 11.1[3]. |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of glutamate decarboxylase B |
could be indirect | ||
|
Protein |
Experiment(s):EBI-887830, EBI-890853 | |||
|
Protein |
Experiment(s):EBI-887830, EBI-888455, EBI-893820 | |||
|
Protein |
Experiment(s):EBI-1139975 | |||
|
Protein |
Experiment(s):EBI-893831 | |||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MDKKQVTDLR SELLDSRFGA KSISTIAESK RFPLHEMRDD VAFQIINDEL YLDGNARQNL ATFCQTWDDE NVHKLMDLSI NKNWIDKEEY PQSAAIDLRC VNMVADLWHA PAPKNGQAVG TNTIGSSEAC MLGGMAMKWR WRKRMEAAGK PTDKPNLVCG PVQICWHKFA RYWDVELREI PMRPGQLFMD PKRMIEACDE NTIGVVPTFG VTYTGNYEFP QPLHDALDKF QADTGIDIDM HIDAASGGFL APFVAPDIVW DFRLPRVKSI SASGHKFGLA PLGCGWVIWR DEEALPQELV FNVDYLGGQI GTFAINFSRP AGQVIAQYYE FLRLGREGYT KVQNASYQVA AYLADEIAKL GPYEFICTGR PDEGIPAVCF KLKDGEDPGY TLYDLSERLR LRGWQVPAFT LGGEATDIVV MRIMCRRGFE MDFAELLLED YKASLKYLSD HPKLQGIAQQ NSFKHT |
|---|---|
| Length |
466 |
| Mol. Wt |
52.669 kDa |
| pI |
5.2 (calculated) |
| Extinction coefficient |
84,340 - 85,590 (calc based on 16 Y, 11 W, and 10 C residues) |
| edit table |
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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Structure
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Structure figures
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| Resource type | Source | Notes/Reference |
|---|---|---|
|
Purification protocol | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 De Biase D et al. (1996) Isolation, overexpression, and biochemical characterization of the two isoforms of glutamic acid decarboxylase from Escherichia coli. Protein Expr Purif 8: 430-8 PubMed EcoliWiki page
- ↑ 6.0 6.1 6.2 6.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 7.0 7.1 7.2 7.3 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
- ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
- ↑ 9.0 9.1 9.2 Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed EcoliWiki page
- ↑ 10.0 10.1 Castanie-Cornet MP et al. (1999) Control of acid resistance in Escherichia coli. J Bacteriol 181: 3525-35 PubMed EcoliWiki page
- ↑ Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
