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galE:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

galE

Mnemonic

Galactose

Synonyms

ECK0748, b0759, JW0742, galD[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

17.03 minutes 

MG1655: 791278..790262
gbrowse_img.php?coord=790262..791278&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 772684..771668
gbrowse_img.php?coord=771668..772684&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 792477..791461
gbrowse_img.php?coord=791461..792477&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 845206..844190
gbrowse_img.php?coord=844190..845206&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 693230..694246
gbrowse_img.php?coord=693230..694246&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

Strain DH10B

S123F

Nonsynonomous mutation

PMID:18245285[2]

ASAP

This is a SNP in E. coli K-12 Strain DH10B

ΔgalE (Keio:JW0742)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8297[4]

galE

PMID:12519969[5]

ASAP

At position 824 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20233

galE45(GalS)

CGSC:5966

galE9(GalS)

PMID:16590575[6]

CGSC:6049

galE28(GalS)

CGSC:6277

galE15(GalS)

CGSC:7813

galE54(GalS)

CGSC:10207

galE66(GalS)

CGSC:11910

galE9999(GalS)

CGSC:28805

galE537(GalS)::Tn10

CGSC:46686

ΔgalE740::kan

PMID:16738554[3]

CGSC:99699


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0742

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAGAGTTCTGGTTACCGGTGG

Primer 2:CCATCGGGATATCCCTGTGGATG

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[4]

est. P1 cotransduction: 69% [8]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[4]

est. P1 cotransduction: 30% [8]
Synonyms:zbi-29::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10362

EchoBASE (EcoliWiki Page)

EchoBASE:EB0357

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0759

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0835

EcoGene (EcoliWiki Page)

EcoGene:EG10362

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GALE

CGSC (EcoliWiki Page)

CGSC:724

ASAP (EcoliWikiPage)

ASAP:ABE-0002575


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Durfee T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J Bacteriol 190: 2597-606 PubMed EcoliWiki page
  3. 3.0 3.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Glasner JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res 31: 147-51 PubMed EcoliWiki page
  6. Kalckar HM et al. (1959) HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES. Proc Natl Acad Sci U S A 45: 1776-86 PubMed EcoliWiki page
  7. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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