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galU:On One Page

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Gene Name	galU (synonyms: ECK1231, b1236, JW1224, ychD, verA)^[1]
Product(s)	GalU product^[1]: glucose-1-phosphate uridylyltransferase product, B1236 product, YchD product
Description	GalU^[2]^[3] Component of UTP-glucose-1-phosphate uridylyltransferase^[2]
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): galU^[2]
Regulation/Activity
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Gene

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Nomenclature

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Standard name	galU
Mnemonic
Synonyms	ECK1231, b1236, JW1224, ychD, verA^[1]
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Notes

Location(s) and DNA Sequence

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Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	27.82 minutes	MG1655: 1290680..1291588	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 1293034..1293942	GBrowse(W3110)	FASTA Dump
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Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

27.82 minutes

MG1655: 1290680..1291588
gbrowse_img.php?coord=1289680..1292588&genome=ecoli&name=MG1655&trackset=0&width=300&.png

EcoCyc

GBrowse(MG1655)

W3110

W3110: 1293034..1293942
gbrowse_img.php?coord=1292034..1294942&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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Feature type	Location	Description
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Notes

Alleles and Phenotypes

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Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔgalU (Keio:JW1224)	deletion	deletion	PMID:16738554^[4]	Shigen CGSC9110^[5]
galU51				CGSC:5226
galU65				CGSC:7342
galU106				CGSC:7925
galU95				CGSC:7942
galU50				CGSC:10034
galU43				CGSC:16267
galU536				CGSC:46657
ΔgalU745::kan			PMID:16738554^[4]	CGSC:101090
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Notes

Genetic Resources

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Resource	Resource Type	Source	Notes/Reference
JW1224	Plasmid clone	Shigen	PMID:16769691^[6] Status:Clone OK Primer 1:GCCGCTGCCATTAATACGAAAGT Primer 2:CCCTTCTTAATGCCCATCTCTTC
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 6% ^[7]
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 63% ^[7] Synonyms:zch-506::Tn10, zci-506::Tn10
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11319
EchoBASE (EcoliWiki Page)	EchoBASE:EB1295
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1236
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C1700
EcoGene (EcoliWiki Page)	EcoGene:EG11319
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:GALU
CGSC (EcoliWiki Page)	CGSC:719
ASAP (EcoliWikiPage)	ASAP:ABE-0004150
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

Standard name	GalU product
Synonyms	glucose-1-phosphate uridylyltransferase product^[1], B1236 product^[2]^[1], YchD product^[2]^[1], GalU product^[2]^[1]
Product description	GalU^[2]^[3]; Component of ; UTP-glucose-1-phosphate uridylyltransferase^[2]^[3]
EC number (for enzymes)	2.7.7.9^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	PMID:16606699^[8]	IPI: Inferred from Physical Interaction	UniProtKB:P37051	F	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0000287	magnesium ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0003983	UTP:glucose-1-phosphate uridylyltransferase activity	GO_REF:0000002 GO_REF:0000003	IEA: Inferred from Electronic Annotation	InterPro:IPR005771 EC:2.7.7.9	F	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0006011	UDP-glucose metabolic process	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005771	P	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0009058	biosynthetic process	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005835	P	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0016740	transferase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0016779	nucleotidyltransferase activity	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR005835 SP_KW:KW-0548	F	Seeded from EcoCyc 12.5 ^[9]	complete
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	required fields missing
	GO:0006012	galactose metabolic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0006006	glucose metabolic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0009242	colanic acid biosynthetic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0016052	carbohydrate catabolic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
Under review	GO:0009269	response to desiccation				P	Seeded from EcoCyc 11.1^[3]. See discussion page.	required field missing
Under review	GO:0042603	capsule				C	Seeded from EcoCyc 11.1^[3]. GalU is not located in the capsule.	required field missing
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Interactions

Partner Type	Partner	Notes	References
Protein	Subunits of UTP-glucose-1-phosphate uridylyltransferase	could be indirect
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Notes

Localization

Compartment	Description	Evidence	Notes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence	at EcoCyc MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI AAGITEIVLV THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP HVTIMQVRQG LAKGLGHAVL CAHPVVGDEP VAVILPDVIL DEYESDLSQD NLAEMIRRFD ETGHSQIMVE PVADVTAYGV VDCKGVELAP GESVPMVGVV EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ LTDAIDMLIE KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI KK
Mol. Wt	32.94 kDa (calc) ^[2]
pI
Extinction coefficient	21430 - 22055 (calc based on 7 Y, 2 W, and 5 C residues)
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Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
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Structure

Structures	Representative Escherichia coli structure: 2E3D\|A 1-302 of 302 residues (E-value: 4.4e-160) (Percent Identity: 100.00) Representative homolog: 2PA4\|A (Corynebacterium glutamicum) 12-292 of 302 residues (E-value: 1.3e-43) (Percent Identity: 37.63) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
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Structures

Representative Escherichia coli structure:
2E3D|A
1-302 of 302 residues (E-value: 4.4e-160) (Percent Identity: 100.00)
Representative homolog:
2PA4|A (Corynebacterium glutamicum)
12-292 of 302 residues (E-value: 1.3e-43) (Percent Identity: 37.63)
View all other structures.

Models

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:GLUC1PURIDYLTRANS-MONOMER
SwissModel (EcoliWiki Page)	SwissModel:P0AEP3
UniProt (EcoliWiki Page)	UniProt:P0AEP3
PFAM (EcoliWiki Page)	PFAM:PF00483
RefSeq (EcoliWiki Page)	RefSeq:NP_415752
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Transcription and Transcriptional Regulation

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Transcription unit(s)
galU
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=1290660..1290700&genome=ecoli&name=MG1655&trackset=Genes_unlabeled+DNA_unlabeled&width=400&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression

Allele Name	Mutation	Phenotype	Reference
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Expression Studies

Type	Reference	Notes
microarray	EcoliGenExpDB:b1236 (EcoliWiki Page)	Summary of data for galU from multiple microarray studies
microarray	GEO Profiles:b1236 (EcoliWiki Page)	NCBI GEO profiles for galU
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Expression Resources

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1290391..1290723) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ03; Well:D2^[10]
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Notes

Accessions Related to galU Expression

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Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG11319
EchoBASE (EcoliWiki Page)	EchoBASE:EB1295
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1236
EcoGene (EcoliWiki Page)	EcoGene:EG11319
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000034883 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G55590 (score: 1.000; bootstrap: 100%) AT2G39770 (score: 1.000; bootstrap: 100%) AT4G30570 (score: 0.662) AT2G04650 (score: 0.194) AT1G74910 (score: 0.194)	From Inparanoid:20070104
Homo sapiens	ENSP00000309092 (score: 1.000; bootstrap: 100%) ENSP00000315925 (score: 0.182)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000019150 (score: 1.000; bootstrap: 100%) ENSMMUP00000023579 (score: 0.131)	From Inparanoid:20070104
Mus musculus	MGI:2660880 (score: 1.000; bootstrap: 100%) MGI:1916330 (score: 0.200)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000025774 (score: 1.000; bootstrap: 100%) ENSPTRP00000022137 (score: 0.147)	From Inparanoid:20070104
Shigella flexneri	GALU	From SHIGELLACYC
E. coli O157	GALU	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF00483
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Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 ^3.8 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
↑ ^9.0 ^9.1 ^9.2 ^9.3 ^9.4 ^9.5 ^9.6 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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Retrieved from "http://ecoliwiki.net/colipedia/index.php/galU:On_One_Page"

galU:On One Page

From EcoliWiki

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Categories

Expression

Overview

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to galU Expression

Notes

Links

Categories

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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