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galU:On One Page

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Standard Name

galU

Gene Synonym(s)

ECK1231, b1236, JW1224, ychD, verA[1]

Product Desc.

GalU[2][3];

Component of UTP-glucose-1-phosphate uridylyltransferase[2][3]

Glucose-1-P uridylyltransferase; also called UDP-glucose pyrophosphorylase[4]

Product Synonyms(s)

glucose-1-phosphate uridylyltransferase[1], B1236[2][1], YchD[2][1], GalU[2][1]

Function from GO

Molecular Function

Cellular Component

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): galU[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(galU)    Textpresso(galU)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

galU

Mnemonic

Galactose

Synonyms

ECK1231, b1236, JW1224, ychD, verA[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

27.82 minutes 

MG1655: 1290680..1291588
gbrowse_img.php?coord=1290680..1291588&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1291251..1292159
gbrowse_img.php?coord=1291251..1292159&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1293034..1293942
gbrowse_img.php?coord=1293034..1293942&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1330921..1331829
gbrowse_img.php?coord=1330921..1331829&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 1180178..1181086
gbrowse_img.php?coord=1180178..1181086&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔgalU (Keio:JW1224)

deletion

deletion

PMID:16738554

Shigen
CGSC9110[5]

galU51

CGSC:5226

galU65

CGSC:7342

galU106

CGSC:7925

galU95

CGSC:7942

galU50

CGSC:10034

galU43

CGSC:16267

galU536

CGSC:46657

ΔgalU745::kan

PMID:16738554

CGSC:101090


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW1224

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCTGCCATTAATACGAAAGT

Primer 2:CCCTTCTTAATGCCCATCTCTTC

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[5]

est. P1 cotransduction: 6% [6]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[5]

est. P1 cotransduction: 63% [6]
Synonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11319

EchoBASE (EcoliWiki Page)

EchoBASE:EB1295

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1236

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1700

EcoGene (EcoliWiki Page)

EcoGene:EG11319

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GALU

CGSC (EcoliWiki Page)

CGSC:719

ASAP (EcoliWikiPage)

ASAP:ABE-0004150


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GalU

Synonyms

glucose-1-phosphate uridylyltransferase[1], B1236[2][1], YchD[2][1], GalU[2][1]

Product description

GalU[2][3];

Component of UTP-glucose-1-phosphate uridylyltransferase[2][3]

Glucose-1-P uridylyltransferase; also called UDP-glucose pyrophosphorylase[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0005515

protein binding

PMID:16606699

IPI: Inferred from Physical Interaction

UniProtKB:P37051

F

Seeded from EcoCyc [7]

complete

GO:0000287

magnesium ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc [7]

complete

GO:0003983

UTP:glucose-1-phosphate uridylyltransferase activity

GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR005771
EC:2.7.7.9

F

Seeded from EcoCyc [7]

complete

GO:0006011

UDP-glucose metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005771

P

Seeded from EcoCyc [7]

complete

GO:0009058

biosynthetic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005835

P

Seeded from EcoCyc [7]

complete

GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc [7]

complete

GO:0016779

nucleotidyltransferase activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR005835
SP_KW:KW-0548

F

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0006012

galactose metabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0006006

glucose metabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009242

colanic acid biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0016052

carbohydrate catabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

Under review

GO:0009269

response to desiccation

P

Seeded from EcoCyc 11.1[3].

See discussion page.

required field missing

Under review

GO:0042603

capsule

C

Seeded from EcoCyc 11.1[3].

GalU is not located in the capsule.

required field missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of UTP-glucose-1-phosphate uridylyltransferase

could be indirect

Protein

purU

PMID:16606699

Experiment(s):EBI-1139268

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI AAGITEIVLV
THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP HVTIMQVRQG LAKGLGHAVL
CAHPVVGDEP VAVILPDVIL DEYESDLSQD NLAEMIRRFD ETGHSQIMVE PVADVTAYGV
VDCKGVELAP GESVPMVGVV EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ
LTDAIDMLIE KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI KK
Length

302

Mol. Wt

32.941 kDa

pI

4.9 (calculated)

Extinction coefficient

21,430 - 22,055 (calc based on 7 Y, 2 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed

UniProt:P0AEP3


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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:GLUC1PURIDYLTRANS-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P0AEP3

UniProt (EcoliWiki Page)

UniProt:P0AEP3

PFAM (EcoliWiki Page)

PFAM:PF00483

RefSeq (EcoliWiki Page)

RefSeq:NP_415752


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

galU

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=1290660..1290700&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b1236 (EcoliWiki Page)

NCBI GEO profiles for galU

microarray

GenExpDB:b1236 (EcoliWiki Page)

Summary of data for galU from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (1290391..1290723) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ03; Well:D2[8]

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Notes

Accessions Related to galU Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11319

EchoBASE (EcoliWiki Page)

EchoBASE:EB1295

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1236

EcoGene (EcoliWiki Page)

EcoGene:EG11319


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Apis mellifera

  • ENSAPMP00000034883 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT3G55590 (score: 1.000; bootstrap: 100%)
  • AT2G39770 (score: 1.000; bootstrap: 100%)
  • AT4G30570 (score: 0.662)
  • AT2G04650 (score: 0.194)
  • AT1G74910 (score: 0.194)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000309092 (score: 1.000; bootstrap: 100%)
  • ENSP00000315925 (score: 0.182)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000019150 (score: 1.000; bootstrap: 100%)
  • ENSMMUP00000023579 (score: 0.131)

From Inparanoid:20070104

Mus musculus

  • MGI:2660880 (score: 1.000; bootstrap: 100%)
  • MGI:1916330 (score: 0.200)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000025774 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000022137 (score: 0.147)

From Inparanoid:20070104

Shigella flexneri

GALU

From SHIGELLACYC

E. coli O157

GALU

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00483


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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