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glnV:Gene Product(s)
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Nomenclature
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| Standard name |
tRNA-Gln(CUG) (Glutamine tRNA2) |
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| Synonyms |
tRNA-Gln(CUG) (Glutamine tRNA2)[1] |
| Product description |
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| EC number (for enzymes) |
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Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
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Notes
supE44 (synonymous to glnV) acts as an amber nonsense mutation (insertion of UAG stop codon) suppressor. This tRNA structural gene mutation, first isolated by Bachmann et al., 1976, causes the transition of the GUC glutamine anticodon to a AUC anticodon. The mutant SupE44 tRNA competes with the amber nonsense mutation tRNA in translation, yielding a phenotype with lower levels of termination of translation.
supE44 's phenotype was determined by Inokuchi et al., 1979. Upon infection with mutant phage containing supE44, higher levels of tRNAGln2 were noted; nuclease digestion and gel analysis yielded similar results to Yaniv and Folk's (1975) tRNAGln2 sequences.
See Bachmann et al., 1976, [2]; Inokuchi et al., 1979, [3]; and Yaniv and Folk, 1975, [4].
Last updated 11/11/07 by user TPian.
Localization
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| Compartment | Description | Evidence | Source | Notes |
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Notes
Structure and Physical Properties
Physical Properties
| Type |
tRNA |
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| Sequence |
UGGGGUAUCG CCAAGCGGUA AGGCACCGGA UUCUGAUUCC GGCAUUCCGA GGUUCGAAUC CUCGUACCCC AGCCA |
| Mol. Wt |
24264.42 |
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Domains/Motifs/Modification Sites See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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Structure
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Structure figures
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| Resource type | Source | Notes/Reference |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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Notes
Links
| Name | URL | Comments |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Bachmann BJ et al. (1976) Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev 40: 116-67 PubMed EcoliWiki page
- ↑ Inokuchi H et al. (1979) Identification of transfer RNA suppressors in Escherichia coli. II. Duplicate genes for tRNA2Gln. J Mol Biol 132: 663-77 PubMed EcoliWiki page
- ↑ Yaniv M & Folk WR (1975) The nucleotide sequences of the two glutamine transfer ribonucleic acids from Escherichia coli. J Biol Chem 250: 3243-53 PubMed EcoliWiki page
