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glnV:On One Page

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Standard Name

glnV

Gene Synonym(s)

ECK0656, b0665, JWR0012, Su2, Su_II, supE, glnValpha, suII[1]

Product Desc.


Product Synonyms(s)

tRNA-Gln(CUG) (Glutamine tRNA2)[1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): metT-leuW-glnUW-metU-glnVX[2], metT

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(glnV)    Textpresso(glnV)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glnV

Mnemonic

Glutamine

Synonyms

ECK0656, b0665, JWR0012, Su2, Su_II, supE, glnValpha, suII[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

15 minutes 

MG1655: 695839..695765
gbrowse_img.php?coord=695765..695839&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 678637..678711
gbrowse_img.php?coord=678637..678711&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 697038..696964
gbrowse_img.php?coord=696964..697038&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 748431..748357
gbrowse_img.php?coord=748357..748431&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 598525..598599
gbrowse_img.php?coord=598525..598599&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

glnV42(AS)

amber (UAG) suppressor

CGSC:4563

glnV44(AS)

amber (UAG) suppressor

PMID:5331503

CGSC:4497

glnV0(AS)

amber (UAG) suppressor

CGSC:10415

glnV33(AS)

amber (UAG) suppressor

CGSC:11602

glnV22(AS)

amber (UAG) suppressor

CGSC:13836

glnV41(AS)

amber (UAG) suppressor

CGSC:13854

glnV93(AS)

amber (UAG) suppressor

CGSC:27776

glnV89(AS)

amber (UAG) suppressor

CGSC:74381


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[3]

est. P1 cotransduction: 17% [4]
Synonyms:zbe-280::Tn10

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[3]

est. P1 cotransduction: 85% [4]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG30029

EchoBASE (EcoliWiki Page)

EchoBASE:EB4192

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0665

EcoGene (EcoliWiki Page)

EcoGene:EG30029

CGSC (EcoliWiki Page)

CGSC:695


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

tRNA-Gln(CUG) (Glutamine tRNA2)

Synonyms

tRNA-Gln(CUG) (Glutamine tRNA2)[1]

Product description


EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence
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Notes

supE44 (synonymous to glnV) acts as an amber nonsense mutation (insertion of UAG stop codon) suppressor. This tRNA structural gene mutation, first isolated by Bachmann et al., 1976, causes the transition of the GUC glutamine anticodon to a AUC anticodon. The mutant SupE44 tRNA competes with the amber nonsense mutation tRNA in translation, yielding a phenotype with lower levels of termination of translation.

supE44 's phenotype was determined by Inokuchi et al., 1979. Upon infection with mutant phage containing supE44, higher levels of tRNAGln2 were noted; nuclease digestion and gel analysis yielded similar results to Yaniv and Folk's (1975) tRNAGln2 sequences.

See Bachmann et al., 1976, [5]; Inokuchi et al., 1979, [6]; and Yaniv and Folk, 1975, [7].

Last updated 11/11/07 by user TPian.

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties

Type

tRNA

Sequence 
UGGGGUAUCG CCAAGCGGUA AGGCACCGGA UUCUGAUUCC GGCAUUCCGA GGUUCGAAUC
CUCGUACCCC AGCCA
Mol. Wt

24264.42

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Domains/Motifs/Modification Sites See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Image couresy of Rfam


Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
  • Representative Escherichia coli structure:
    1QTQ|B
    1-75 of 75 residues (E-value: 6e-13) (Percent Identity: 100.00)
  • Representative homolog:
    3KNH|W (Escherichia coli DH1)
    2-75 of 75 residues (E-value: 1e-12) (Percent Identity: 100.00)
  • View the PDB Table for glnV
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes
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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

metT-leuW-glnUW-metU-glnVX

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=695819..695859&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0665 (EcoliWiki Page)

NCBI GEO profiles for glnV

microarray

GenExpDB:b0665 (EcoliWiki Page)

Summary of data for glnV from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to glnV Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG30029

EchoBASE (EcoliWiki Page)

EchoBASE:EB4192

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0665

EcoGene (EcoliWiki Page)

EcoGene:EG30029


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Notes

Links

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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF01360

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. ↑ Bachmann BJ et al. (1976) Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev 40: 116-67 PubMed EcoliWiki page
  6. ↑ Inokuchi H et al. (1979) Identification of transfer RNA suppressors in Escherichia coli. II. Duplicate genes for tRNA2Gln. J Mol Biol 132: 663-77 PubMed EcoliWiki page
  7. ↑ Yaniv M & Folk WR (1975) The nucleotide sequences of the two glutamine transfer ribonucleic acids from Escherichia coli. J Biol Chem 250: 3243-53 PubMed EcoliWiki page

Categories

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