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gltI:On One Page

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Standard Name

gltI

Gene Synonym(s)

ECK0648, b0655, JW5092, ybeJ, yzzK[1]

Product Desc.

putative periplasmic binding transport protein[2]; GltI[3];

Component of glutamate ABC transporter[2]; GltIJKL glutamate ABC transporter[3]

Periplasmic glutamate-aspartate binding protein[4]

Product Synonyms(s)

glutamate and aspartate transporter subunit[1], periplasmic-binding component of ABC superfamily[1], B0655[2][1], YzzK[2][1], YbeJ[2][1], GltI[2][1], GltBP[3][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): gltIJKL[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(gltI)    Textpresso(gltI)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

gltI

Mnemonic

Glutamate

Synonyms

ECK0648, b0655, JW5092, ybeJ, yzzK[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

14.79 minutes 

MG1655: 686970..686062
gbrowse_img.php?coord=686062..686970&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 668979..668071
gbrowse_img.php?coord=668071..668979&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 688169..687261
gbrowse_img.php?coord=687261..688169&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 626302..625394
gbrowse_img.php?coord=625394..626302&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

DH10B

DH10B: 739562..738654
gbrowse_img.php?coord=738654..739562&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 588822..589730
gbrowse_img.php?coord=588822..589730&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔgltI (Keio:JW5092)

deletion

deletion

PMID:16738554

Shigen
CGSC11169[5]

ΔgltI788::kan

PMID:16738554

CGSC:101202


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW5092

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCAATTACGTAAACCTGCCAC

Primer 2:CCGTTCAGTGCCTTGTCATTCGG

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[5]

est. P1 cotransduction: 28% [6]
Synonyms:zbe-280::Tn10

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[5]

est. P1 cotransduction: 60% [6]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G6359

EchoBASE (EcoliWiki Page)

EchoBASE:EB2562

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0655

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0739

EcoGene (EcoliWiki Page)

EcoGene:EG12700

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YBEJ

ASAP (EcoliWikiPage)

ASAP:ABE-0002241


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GltI

Synonyms

glutamate and aspartate transporter subunit[1], periplasmic-binding component of ABC superfamily[1], B0655[2][1], YzzK[2][1], YbeJ[2][1], GltI[2][1], GltBP[3][1]

Product description

putative periplasmic binding transport protein[2]; GltI[3];

Component of glutamate ABC transporter[2]; GltIJKL glutamate ABC transporter[3]

Periplasmic glutamate-aspartate binding protein[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0005215

transporter activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001638

F

Seeded from EcoCyc [7]

complete

GO:0006810

transport

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001638
SP_KW:KW-0813

P

Seeded from EcoCyc [7]

complete

GO:0006865

amino acid transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0029

P

Seeded from EcoCyc [7]

complete

GO:0030288

outer membrane-bounded periplasmic space

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001638

C

Seeded from EcoCyc [7]

complete

GO:0042597

periplasmic space

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0006537

glutamate biosynthetic process

P

Seeded from EcoCyc 10.6[2].

required fields missing

GO:0015810

aspartate transport

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005313

L-glutamate transmembrane transporter activity

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0016595

glutamate binding

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0016597

amino acid binding

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0015813

glutamate transport

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0015183

L-aspartate transmembrane transporter activity

F

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of glutamate ABC transporter

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

Periplasm

PMID:8740179

EchoLocation:gltI


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MQLRKPATAI LALALSAGLA QADDAAPAAG STLDKIAKNG VIVVGHRESS VPFSYYDNQQ
KVVGYSQDYS NAIVEAVKKK LNKPDLQVKL IPITSQNRIP LLQNGTFDFE CGSTTNNVER
QKQAAFSDTI FVVGTRLLTK KGGDIKDFAN LKDKAVVVTS GTTSEVLLNK LNEEQKMNMR
IISAKDHGDS FRTLESGRAV AFMMDDALLA GERAKAKKPD NWEIVGKPQS QEAYGCMLRK
DDPQFKKLMD DTIAQVQTSG EAEKWFDKWF KNPIPPKNLN MNFELSDEMK ALFKEPNDKA LN
Length

302

Mol. Wt

33.42 kDa

pI

8.8 (calculated)

Extinction coefficient

23,950 - 24,200 (calc based on 5 Y, 3 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

motif

1-22

UniProt Manual:Signal Peptides

UniProt:P37902


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Structure
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Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G6359-MONOMER

UniProt (EcoliWiki Page)

UniProt:P37902

PFAM (EcoliWiki Page)

PFAM:PF00497

RefSeq (EcoliWiki Page)

RefSeq:NP_415188


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

gltIJKL

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=686950..686990&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0655 (EcoliWiki Page)

NCBI GEO profiles for gltI

microarray

GenExpDB:b0655 (EcoliWiki Page)

Summary of data for gltI from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to gltI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G6359

EchoBASE (EcoliWiki Page)

EchoBASE:EB2562

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0655

EcoGene (EcoliWiki Page)

EcoGene:EG12700


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Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Apis mellifera

  • ENSAPMP00000032943 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000047113 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

YBEJ

From SHIGELLACYC

E. coli O157

YBEJ

From ECOO157CYC

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Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00497


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Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

Categories

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