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gsk:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

gsk

Mnemonic

Guanosine kinase

Synonyms

ECK0471, b0477, JW0466[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

10.76 minutes 

MG1655: 499349..500653
gbrowse_img.php?coord=499349..500653&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 472192..473496
gbrowse_img.php?coord=472192..473496&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 499349..500653
gbrowse_img.php?coord=499349..500653&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 438680..439984
gbrowse_img.php?coord=438680..439984&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 402108..403412
gbrowse_img.php?coord=402108..403412&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

Δgsk (Keio:JW0466)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8618[3]

gsk-3055::Tn10

Insertion at 500,025 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12154 = CGSC7341[3]

Synonyms: zbb-30-55::TnlO, zba-3055::Tn10

gsk-11

CGSC:7467

gsk-3055::Tn10

PMID:2540407[5] PMID:9829956[6]

CGSC:39377

Δgsk-759::kan

PMID:16738554[2]

CGSC:101264


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0466

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAAATTTCCCGGTAAACGTAA

Primer 2:CCACGATCCCAGTAAGACTCTTC

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[3]

est. P1 cotransduction: 18% [4]
Synonyms:zba-3054::Tn10

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[3]

est. P1 cotransduction: 8% [4]
Synonyms:purE79::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11102

EchoBASE (EcoliWiki Page)

EchoBASE:EB1094

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0477

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0597

EcoGene (EcoliWiki Page)

EcoGene:EG11102

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GSK

CGSC (EcoliWiki Page)

CGSC:658

ASAP (EcoliWikiPage)

ASAP:ABE-0001655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. Singer M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev 53: 1-24 PubMed EcoliWiki page
  6. Nichols BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J Bacteriol 180: 6408-11 PubMed EcoliWiki page
  7. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page

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