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Standard Name

guaA

Gene Synonym(s)

ECK2503, b2507, JW2491[1]

Product Desc.

GuaA[2]; GMP synthetase[3];

Component of GMP-SYN-CPLX[2]

GMP synthase[4]

Product Synonyms(s)

GMP synthetase (glutamine aminotransferase)[1], B2507[2][1], GuaA[2][1]

Function from GO

Molecular Function

Biological Process

Cellular Component

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): guaBA[2], OP00288

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(guaA)    Textpresso(guaA)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

guaA

Mnemonic

Guanine

Synonyms

ECK2503, b2507, JW2491[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

56.66 minutes, 56.66 minutes 

MG1655: 2630557..2628980
gbrowse_img.php?coord=2628980..2630557&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2553354..2551777
gbrowse_img.php?coord=2551777..2553354&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2631191..2629614
gbrowse_img.php?coord=2629614..2631191&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2722322..2720745
gbrowse_img.php?coord=2720745..2722322&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2514785..2516362
gbrowse_img.php?coord=2514785..2516362&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔguaA (Keio:JW2491)

deletion

deletion

PMID:16738554

Shigen

guaA21

CGSC:11495


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW2491

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACGGAAAACATTCATAAGCA

Primer 2:CCTTCCCACTCAATGGTAGCTGG

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[5]

est. P1 cotransduction: 24% [6]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[5]

est. P1 cotransduction: 25% [6]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10420

EchoBASE (EcoliWiki Page)

EchoBASE:EB0415

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2507

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3026

EcoGene (EcoliWiki Page)

EcoGene:EG10420

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GUAA

CGSC (EcoliWiki Page)

CGSC:657

ASAP (EcoliWikiPage)

ASAP:ABE-0008254


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GuaA

Synonyms

GMP synthetase (glutamine aminotransferase)[1], B2507[2][1], GuaA[2][1]

Product description

GuaA[2]; GMP synthetase[3];

Component of GMP-SYN-CPLX[2]

GMP synthase[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0003922

GMP synthase (glutamine-hydrolyzing) activity

PMID:9890911

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0003921

GMP synthase activity

PMID:17103135

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc [8]

complete

GO:0005515

protein binding

PMID:16606699

IPI: Inferred from Physical Interaction

UniProtKB:P23843

F

Seeded from EcoCyc [8]

complete

GO:0000166

nucleotide binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc [8]

complete

GO:0003922

GMP synthase (glutamine-hydrolyzing) activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR001674
InterPro:IPR004739
EC:6.3.5.2
HAMAP:MF_00344

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0004808

tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004506

F

Seeded from EcoCyc [8]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR001674
InterPro:IPR004739
SP_KW:KW-0067
HAMAP:MF_00344

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0005737

cytoplasm

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004506

C

Seeded from EcoCyc [8]

complete

GO:0006177

GMP biosynthetic process

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR001674
InterPro:IPR004739
SP_KW:KW-0332
HAMAP:MF_00344

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0006541

glutamine metabolic process

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001317
InterPro:IPR011702
SP_KW:KW-0315

P

Seeded from EcoCyc [8]

complete

GO:0008033

tRNA processing

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004506

P

Seeded from EcoCyc [8]

complete

GO:0006177

GMP biosynthetic process

PMID:4353875

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0003824

catalytic activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000991
InterPro:IPR001317
InterPro:IPR006220
InterPro:IPR011702

F

Seeded from EcoCyc [8]

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [8]

complete

GO:0009058

biosynthetic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006220

P

Seeded from EcoCyc [8]

complete

GO:0016874

ligase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc [8]

complete

GO:0015949

nucleobase, nucleoside and nucleotide interconversion

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0003922

GMP synthase (glutamine-hydrolyzing) activity

PMID:9890911

IDA

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0006177

GMP biosynthetic process

PMID:4353875

IMP

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0003921

GMP synthase activity

PMID:17103135

IDA

F

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of GMP-SYN-CPLX

could be indirect

Protein

nadE

PMID:16606699

Experiment(s):EBI-1143063

Protein

phnN

PMID:16606699

Experiment(s):EBI-1143063

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MTENIHKHRI LILDFGSQYT QLVARRVREL GVYCELWAWD VTEAQIRDFN PSGIILSGGP
ESTTEENSPR APQYVFEAGV PVFGVCYGMQ TMAMQLGGHV EASNEREFGY AQVEVVNDSA
LVRGIEDALT ADGKPLLDVW MSHGDKVTAI PSDFITVAST ESCPFAIMAN EEKRFYGVQF
HPEVTHTRQG MRMLERFVRD ICQCEALWTP AKIIDDAVAR IREQVGDDKV ILGLSGGVDS
SVTAMLLHRA IGKNLTCVFV DNGLLRLNEA EQVLDMFGDH FGLNIVHVPA EDRFLSALAG
ENDPEAKRKI IGRVFVEVFD EEALKLEDVK WLAQGTIYPD VIESAASATG KAHVIKSHHN
VGGLPKEMKM GLVEPLKELF KDEVRKIGLE LGLPYDMLYR HPFPGPGLGV RVLGEVKKEY
CDLLRRADAI FIEELRKADL YDKVSQAFTV FLPVRSVGVM GDGRKYDWVV SLRAVETIDF
MTAHWAHLPY DFLGRVSNRI INEVNGISRV VYDISGKPPA TIEWE
Length

525

Mol. Wt

58.678 kDa

pI

5.2 (calculated)

Extinction coefficient

64,860 - 65,735 (calc based on 14 Y, 8 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Modification Site

357

phosphorylation site at S357

probability greater than 75%

PMID:17938405

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:GMP-SYN-MONOMER

UniProt (EcoliWiki Page)

UniProt:P04079

PFAM (EcoliWiki Page)

PFAM:PF00117

RefSeq (EcoliWiki Page)

RefSeq:NP_417002


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

guaBA

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2630537..2630577&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b2507 (EcoliWiki Page)

NCBI GEO profiles for guaA

microarray

GenExpDB:b2507 (EcoliWiki Page)

Summary of data for guaA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (2630460..2630677) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ05; Well:F11[9]

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Notes

Accessions Related to guaA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10420

EchoBASE (EcoliWiki Page)

EchoBASE:EB0415

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2507

EcoGene (EcoliWiki Page)

EcoGene:EG10420


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000012372 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000013216 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT1G63660 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000017301 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00025948 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00010912 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000012916 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000011809 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-040426-1503 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040425-2437 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0215334 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0000239 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA21637-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000016711 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000295920 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000009601 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000019674 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:2448526 (score: 1.000; bootstrap: 100%)
  • MGI:3643850 (score: 0.051)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000026780 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YMR217W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAP7G52c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000159883 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00005031001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

GUAA

From SHIGELLACYC

E. coli O157

GUAA

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00117


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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