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hokC:On One Page

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Standard Name

hokC

Gene Synonym(s)

ECK0017, b4412, JW5002, gef[1]

Product Desc.

HokC, Gef toxin; interferes with membrane function when in excess[2][3];

Component of Gef dimer[2][3]

Product Synonyms(s)

toxic membrane protein, small[1], B4412[2][1], Gef[2][1], HokC[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): hokC[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(hokC)    Textpresso(hokC)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hokC

Mnemonic

Host killing

Synonyms

ECK0017, b4412, JW5002, gef[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

0.36 minutes 

MG1655: 16903..16751
gbrowse_img.php?coord=16751..16903&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 16903..16751
gbrowse_img.php?coord=16751..16903&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 16903..16751
gbrowse_img.php?coord=16751..16903&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 16751..16903
gbrowse_img.php?coord=16751..16903&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔhokC (Keio:JW5002)

deletion

deletion

PMID:16738554

Shigen

hokCC15S

C15S

No action on toxicity

seeded from UniProt:P0ACG4

hokCT25K

T25K

Non-toxic

seeded from UniProt:P0ACG4

hokCL29P

L29P

Non-toxic

seeded from UniProt:P0ACG4

hokCC30S

C30S

Prevents the formation of dimer

seeded from UniProt:P0ACG4

hokCV41A

V41A

Non-toxic

seeded from UniProt:P0ACG4

hokCS49E

S49E

Non-toxic

seeded from UniProt:P0ACG4

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW5002

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAGCAGCATAAGGCGATGAT

Primer 2:CCtTCGGATTCGTAAGCCGTGAA

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[4]

est. P1 cotransduction: 55% [5]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[4]

est. P1 cotransduction: 57% [5]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G0-9563

EchoBASE (EcoliWiki Page)

EchoBASE:EB0368

EcoGene (EcoliWiki Page)

EcoGene:EG10373

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GEF

ASAP (EcoliWikiPage)

ASAP:ABE-0047278


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HokC

Synonyms

toxic membrane protein, small[1], B4412[2][1], Gef[2][1], HokC[2][1]

Product description

HokC, Gef toxin; interferes with membrane function when in excess[2][3];

Component of Gef dimer[2][3]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of Gef dimer

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MKQHKAMIVA LIVICITAVV AALVTRKDLC EVHIRTGQTE VAVFTAYESE
Length

50

Mol. Wt

5.5 kDa

pI

7.3 (calculated)

Extinction coefficient

1,490 - 1,740 (calc based on 1 Y, W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:MONOMER0-1564

UniProt (EcoliWiki Page)

UniProt:P0ACG4

PFAM (EcoliWiki Page)

PFAM:PF01848

RefSeq (EcoliWiki Page)

RefSeq:YP_025292

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GEF-MONOMER


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

hokC

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=16883..16923&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to hokC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G0-9563

EchoBASE (EcoliWiki Page)

EchoBASE:EB0368

EcoGene (EcoliWiki Page)

EcoGene:EG10373


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Notes

Links

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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF01848


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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