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Component of [[:Category:Complex:EcoKI restriction-modification system|EcoKI restriction-modification system]]
Specificity determinant for HsdM and HsdR
|Function from GO|
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The restriction-modification complex EcoKI is comprised of three polypeptide subunits coded for by the genes hsdS, hsdM, and hsdR. HsdS is the polypeptide that directly interacts with DNA through the use of two target recognition domain (TRD) sequences first isolated by Fuller-Pace and Murray. HsdS recognizes and binds to the nucleotide sequence 5' AAC(N)6GTGC. Fuller-Pace and Murray determined this sequence of the TRDs in hsdS after recombining two homologous hsdS genes from a family of restriction-modification complexes.
Substitution of Trp212 to Arg alters the protein-protein interaction with HsdM, in turn effecting the DNA binding of the molecule; DNA binding in the mutant is restored by HsdR binding.
- Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- Fuller-Pace, FV & Murray, NE (1986) Two DNA recognition domains of the specificity polypeptides of a family of type I restriction enzymes. Proc. Natl. Acad. Sci. U.S.A. 83 9368-72 PubMed EcoliWiki page